FastQCFastQC Report
Thu 9 Feb 2017
SRR3211919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4290384
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC30750.022.07967437
TACTTAC12800.016.76562531
TCGTTAC16200.016.2160523
TATACGA803.3852612E-416.18752
TCTTATA51900.015.57707237
CTCGTTA18950.015.52242822
CGTTACG13400.015.32462624
TTATACT4900.014.724494
TATACCG2301.8189894E-1214.4782615
TATACTG5900.014.4237285
GTACTTA15850.014.35646630
GTATAGA4650.014.322581
ACTTACA12800.014.30859532
TACGGAT29500.014.17288127
ATCTCGT22950.013.94553420
TCTCGTT22400.013.70982221
GATCTCG23800.013.68067319
ACGGATG30850.013.67260828
CGGTACG1254.850413E-513.32000124
TAAGACT6250.013.3200014
Produced by FastQC (version 0.11.5)