FastQCFastQC Report
Thu 9 Feb 2017
SRR3211918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4512216
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51250.11358055554078084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC40000.020.44250137
TACTTAC17600.016.5028431
CTCGTTA17550.016.23361822
GGTATCA44150.016.1744041
TCGTTAC16150.016.03715123
CGCGGTA2600.015.65384622
AGTGTAC21900.015.28995527
TCTTATA67400.015.23368137
CGTTACG13450.015.13011324
GTACTTA20150.014.68982730
GATCTCG21800.014.08715519
TACGGAT28000.014.00714327
GTGTACT21800.014.00229428
ACTTACA14150.013.98939932
TCTCGTT20550.013.68369821
ATTACGG12650.013.45454525
ATCTCGT22350.013.40939720
ACGGATG29050.013.30981128
TTCGTTA2102.046363E-913.21428522
GGGCGTC16250.013.09230711
Produced by FastQC (version 0.11.5)