FastQCFastQC Report
Thu 9 Feb 2017
SRR3211916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782143
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99600.12798531201495528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC66900.020.7399137
TATACCG3650.017.7397255
GGTATCA60950.016.7547171
GTAACGT5150.016.52427126
TCTTATA117100.015.68787337
CTCTTAT179800.012.61457337
TTAGCCG7100.012.5070434
GGACCGT9300.012.134416
TCTATAC9700.012.0154643
TAACGTC7500.011.59333427
TATACTG12650.011.5533595
GGTAACG7550.011.51655625
CGGTTAC3700.011.528
ACGTCAA8350.011.29940129
GTATCAA89500.011.2446942
AACGCAG112850.011.0983615
CGGGTAA7550.011.02648923
TAGCCGG8850.010.8700565
GCGGGTA8050.010.80124322
TAGACAG14250.010.6456145
Produced by FastQC (version 0.11.5)