FastQCFastQC Report
Thu 9 Feb 2017
SRR3211915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387145
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25850.021.11218637
TACTTAC7800.016.60256431
TCTTATA46150.016.15492837
AGTGTAC10800.014.73148127
GTCTTAT3050.014.5573771
GTATTAG3250.014.2307691
TACGGAT10550.014.02843627
GTAATAC3300.014.0151513
TAATGCG800.00630196213.8754
ACTATCG3100.013.72580711
GTACTTA9800.013.40306230
TAGCGCT1254.8498245E-513.3200014
TTACGGA11450.012.92576526
ATTAGAC2152.906745E-912.9069773
TCTATAC4200.012.7738093
ACTTACA7100.012.76760632
ACTAAAC3500.012.6857153
GTGTACT10950.012.67123228
GTTACGG9950.012.64321625
TAGACAG6650.012.5187975
Produced by FastQC (version 0.11.5)