FastQCFastQC Report
Thu 9 Feb 2017
SRR3211914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511502
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20150.019.64764237
TCTTATA36800.017.3437537
ACTATCG2600.014.94230711
GTACCGT2900.014.0344836
ACTCCGT3000.013.5666676
TTTATAC4800.013.4895833
CTCTTAT58100.013.30981137
TAGACAG6450.012.9069775
TACTTAC4200.012.77380931
CAATACT4500.012.7444454
CGCGGTA1653.8092421E-612.33333322
TATACCC4200.012.3333335
CGTTACG2901.2732926E-1112.12068924
GTCTTAC3700.012.01
TATACTG5300.011.8679255
CTCGTTA3900.011.85897422
TATACTC3153.6379788E-1211.7460325
GTATAAT3351.8189894E-1211.5970151
TCGTTAC4000.011.56250123
TCCGTGC4650.011.5376348
Produced by FastQC (version 0.11.5)