FastQCFastQC Report
Thu 9 Feb 2017
SRR3211912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070974
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21700.022.4216637
TCTTATA41700.018.1007237
GGTATCA17750.017.926761
TACTTAC5900.017.55932231
CTAGACA3000.015.4166674
TATACCG1201.9379695E-615.4166665
GTCTTAT2750.014.7999991
TAACACG1051.6570538E-414.0952384
GTACTTA7600.013.63157930
ACTAGAC2501.0913936E-1113.323
CTCTTAT66300.013.30995537
CGCGCTA850.00940941113.05882436
GTACTAA850.00940941113.0588241
GTATCAA24700.012.957492
GTAACGT1307.0052374E-512.80769326
GGAGTGT12700.012.6732286
AGTGTAC9500.012.65789527
GTATAAA2951.8189894E-1212.5423731
TCAATAC3450.012.3333333
CGTTAGA1257.224742E-411.842
Produced by FastQC (version 0.11.5)