##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070974
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37969354348164	33.0	31.0	34.0	31.0	34.0
2	32.51524304666858	34.0	31.0	34.0	31.0	34.0
3	32.588048612590015	34.0	31.0	34.0	31.0	34.0
4	36.104604923389125	37.0	35.0	37.0	35.0	37.0
5	35.97573733935878	37.0	35.0	37.0	35.0	37.0
6	35.994417731963864	37.0	35.0	37.0	35.0	37.0
7	35.976444932454655	37.0	35.0	37.0	35.0	37.0
8	35.949848484552454	37.0	35.0	37.0	35.0	37.0
9	37.784028129186375	39.0	38.0	39.0	35.0	39.0
10	37.63243615869102	39.0	37.0	39.0	35.0	39.0
11	37.74547912160767	39.0	37.0	39.0	35.0	39.0
12	37.6700577732016	39.0	37.0	39.0	35.0	39.0
13	37.73030771344857	39.0	37.0	39.0	35.0	39.0
14	39.07641940309492	40.0	38.0	41.0	36.0	41.0
15	39.034766494278365	40.0	38.0	41.0	36.0	41.0
16	38.996534649918885	40.0	38.0	41.0	36.0	41.0
17	38.97372006405785	40.0	38.0	41.0	36.0	41.0
18	38.95286869898605	40.0	38.0	41.0	36.0	41.0
19	38.959405387346166	40.0	38.0	41.0	36.0	41.0
20	38.909551822972126	40.0	38.0	41.0	35.0	41.0
21	38.896609674976084	40.0	38.0	41.0	35.0	41.0
22	38.86733557496742	40.0	38.0	41.0	35.0	41.0
23	38.80341871992404	40.0	38.0	41.0	35.0	41.0
24	38.77282647134101	40.0	38.0	41.0	35.0	41.0
25	38.71409429060617	40.0	38.0	41.0	35.0	41.0
26	38.61455258168907	40.0	38.0	41.0	35.0	41.0
27	38.5295740048597	40.0	38.0	41.0	34.0	41.0
28	38.465331520227785	40.0	38.0	41.0	34.0	41.0
29	38.40351269662329	40.0	38.0	41.0	34.0	41.0
30	38.35470993893143	40.0	38.0	41.0	34.0	41.0
31	38.30713317664038	40.0	38.0	41.0	34.0	41.0
32	38.24706982214763	40.0	38.0	41.0	34.0	41.0
33	38.2083456258503	40.0	38.0	41.0	34.0	41.0
34	38.15680953339234	40.0	38.0	41.0	34.0	41.0
35	38.108128235537	40.0	38.0	41.0	33.0	41.0
36	38.04165811888997	40.0	38.0	41.0	33.0	41.0
37	38.02449939335207	40.0	38.0	41.0	33.0	41.0
38	37.96951325540366	40.0	38.0	41.0	33.0	41.0
39	37.92521981625374	40.0	37.0	41.0	33.0	41.0
40	37.88505894221182	40.0	37.0	41.0	33.0	41.0
41	37.8063604576268	40.0	37.0	41.0	33.0	41.0
42	37.72861020640357	40.0	37.0	41.0	33.0	41.0
43	36.751382786047685	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	0.0
12	0.0
13	2.0
14	1.0
15	2.0
16	12.0
17	23.0
18	67.0
19	128.0
20	312.0
21	539.0
22	1124.0
23	1988.0
24	3320.0
25	5343.0
26	7922.0
27	11373.0
28	16318.0
29	22727.0
30	30587.0
31	40896.0
32	54727.0
33	72901.0
34	100804.0
35	143589.0
36	217838.0
37	375018.0
38	874453.0
39	1088955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29577521659252	16.650678253869945	12.275681917202816	27.777864612334717
2	19.25698491748872	18.994625158011758	34.72754897957456	27.020840944924966
3	20.496526509179173	20.611017872505595	28.839449633894652	30.05300598442058
4	15.847382621930372	14.43877414787621	32.53479189338627	37.17905133680715
5	16.816358588512962	34.16020454748233	33.13736944695722	15.886067417047489
6	37.98407280556592	32.352341634934064	14.341769093453738	15.321816466046279
7	31.59675073771383	28.724697766897407	19.746243374251947	19.932308121136813
8	28.095874468491104	33.91148866776469	18.61914167948019	19.373495184264016
9	28.254521204021916	13.706628581029992	17.183310571825096	40.855539643122995
10	17.404413062435566	25.41473812542861	32.203724290729916	24.977124521405912
11	38.89362137224216	19.9274888032266	19.80560564824059	21.37328417629065
12	22.931161253726017	22.861443958822182	28.821018999183973	25.386375788267827
13	32.639058487632916	18.28045434445228	22.60325225807838	26.477234909836422
14	23.75262701670545	18.47589722348675	23.675452804224328	34.09602295558347
15	27.177175710377227	25.403992349007186	20.710986156183672	26.70784578443191
16	26.39817204574184	24.33638318005949	22.750729898722685	26.51471487547599
17	26.04538494953067	23.937096178606527	23.47864228091804	26.53887659094476
18	26.241120895194815	23.87184000906553	23.88900068838095	25.99803840735871
19	27.481867316362823	23.541716732215903	23.602446650476363	25.37396930094491
20	26.821816140416686	23.184761577271576	23.578903631225792	26.414518651085945
21	26.36026876163719	23.697237423696844	24.00010550398668	25.94238831067928
22	27.171086437071757	23.123022207286677	23.10556846134158	26.600322894299982
23	26.542393390500862	23.33481169166525	23.623547447812975	26.499247470020915
24	26.291788859169763	23.124878295941286	23.697074608902582	26.886258235986364
25	26.770855109812064	22.92702575795171	23.52970100039922	26.772418131837
26	26.34460597842899	23.265582841144212	23.844715064341152	26.54509611608565
27	26.954379945906414	23.545135842895444	23.422471176896973	26.07801303430117
28	26.386091188007455	23.952433332226192	23.060143133741935	26.60133234602442
29	26.365543960971337	23.98900153501788	23.491927968129982	26.1535265358808
30	27.051938570629385	23.545168405854298	23.166558883272863	26.236334140243457
31	26.406149970660774	23.200228982726653	23.715602932489823	26.678018114122747
32	25.92949337897358	23.551550745789445	23.713551466082098	26.80540440915488
33	26.042747349863593	23.135721761239267	23.879817933984462	26.941712954912678
34	26.425264427507365	23.01009386598519	24.469174926261182	26.09546678024627
35	26.27944749776455	22.986485720816912	24.17350325987781	26.560563521540725
36	25.84495993779172	22.5452250654027	24.779369672292894	26.83044532451268
37	25.553260952388396	22.08735730097357	25.199171337823113	27.160210408814923
38	25.51903728263411	21.77608797729971	25.53955194671137	27.16532279335481
39	24.82993994739128	21.643296231098017	26.60784493779498	26.91891888371572
40	25.456776905307564	21.237659452668762	26.814098719168577	26.491464922855094
41	24.50587338088828	21.418221059507506	27.280042097393203	26.79586346221101
42	23.847775982473312	21.647366600954616	27.658130612633	26.846726803939074
43	23.387465996130217	21.31340089496036	27.624721016850028	27.67441209205939
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	27.0
2	29.0
3	73.0
4	117.0
5	117.0
6	161.5
7	206.0
8	196.5
9	187.0
10	264.5
11	342.0
12	342.0
13	633.5
14	925.0
15	1332.5
16	1740.0
17	1864.5
18	1989.0
19	1989.0
20	2579.5
21	3170.0
22	3934.5
23	4699.0
24	6646.5
25	8594.0
26	8594.0
27	11467.5
28	14341.0
29	18128.0
30	21915.0
31	27696.5
32	33478.0
33	33478.0
34	42114.5
35	50751.0
36	61708.5
37	72666.0
38	86915.0
39	101164.0
40	101164.0
41	117882.0
42	134600.0
43	155024.5
44	175449.0
45	200498.0
46	225547.0
47	225547.0
48	250218.0
49	274889.0
50	293772.5
51	312656.0
52	315484.0
53	318312.0
54	318312.0
55	307837.5
56	297363.0
57	282045.0
58	266727.0
59	248164.0
60	229601.0
61	229601.0
62	202840.0
63	176079.0
64	149699.5
65	123320.0
66	102592.0
67	81864.0
68	81864.0
69	67210.5
70	52557.0
71	43154.0
72	33751.0
73	27854.0
74	21957.0
75	21957.0
76	17626.0
77	13295.0
78	10686.0
79	8077.0
80	6283.5
81	4490.0
82	4490.0
83	3365.0
84	2240.0
85	1687.5
86	1135.0
87	818.5
88	502.0
89	502.0
90	328.0
91	154.0
92	102.0
93	50.0
94	33.0
95	16.0
96	16.0
97	10.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3070974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.83567885573419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.20941170902042	53.117292836315954
2	9.109422644572732	11.630123570001954
3	2.8408329287188283	5.440394955614698
4	1.3957500395668083	3.563946051546603
5	0.8410124567872364	2.6843300552571026
6	0.5468033601333183	2.0943338216824126
7	0.3832632723292129	1.71261098187238
8	0.2986041329747102	1.5249278030054838
9	0.20123502873163526	1.15613772017734
>10	1.0993877116590216	12.348832727653775
>50	0.05338992768578275	2.3132622863110877
>100	0.020427553565210015	2.208175031124301
>500	4.0820822682495093E-4	0.17133298890336615
>1k	5.1026028244205764E-5	0.03429917053351621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
2	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
3	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
4	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
5	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
6	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
7	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
8	0.0	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-5
9	0.0	0.0	0.0	6.512591770558787E-5	3.2562958852793935E-5
10	0.0	0.0	0.0	1.3025183541117574E-4	6.512591770558787E-5
11	0.0	0.0	0.0	1.6281479426396967E-4	6.512591770558787E-5
12	0.0	0.0	0.0	1.6281479426396967E-4	6.512591770558787E-5
13	0.0	0.0	0.0	1.6281479426396967E-4	9.76888765583818E-5
14	0.0	0.0	0.0	1.6281479426396967E-4	1.3025183541117574E-4
15	0.0	0.0	0.0	1.953777531167636E-4	1.6281479426396967E-4
16	0.0	0.0	3.2562958852793935E-5	1.953777531167636E-4	1.6281479426396967E-4
17	0.0	0.0	3.2562958852793935E-5	2.605036708223515E-4	1.953777531167636E-4
18	0.0	0.0	3.2562958852793935E-5	3.2562958852793935E-4	2.605036708223515E-4
19	0.0	0.0	3.2562958852793935E-5	3.907555062335272E-4	2.605036708223515E-4
20	0.0	0.0	3.2562958852793935E-5	4.558814239391151E-4	3.2562958852793935E-4
21	0.0	0.0	3.2562958852793935E-5	5.861332593502908E-4	3.5819254738073326E-4
22	0.0	0.0	3.2562958852793935E-5	8.791998890254363E-4	3.5819254738073326E-4
23	0.0	0.0	3.2562958852793935E-5	0.0011071406009949937	4.88444382791909E-4
24	0.0	0.0	3.2562958852793935E-5	0.0021165923254316057	4.88444382791909E-4
25	0.0	0.0	3.2562958852793935E-5	0.002832977420193072	5.21007341644703E-4
26	0.0	0.0	3.2562958852793935E-5	0.004917006786771884	5.535703004974969E-4
27	0.0	0.0	3.2562958852793935E-5	0.018495760628386954	5.535703004974969E-4
28	0.0	0.0	3.2562958852793935E-5	0.06662381381281639	5.535703004974969E-4
29	0.0	0.0	3.2562958852793935E-5	0.14047660449095303	5.535703004974969E-4
30	0.0	0.0	3.2562958852793935E-5	0.23718859228375103	5.535703004974969E-4
31	0.0	0.0	3.2562958852793935E-5	0.6627213385720622	5.861332593502908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2170	0.0	22.42166	37
TCTTATA	4170	0.0	18.10072	37
GGTATCA	1775	0.0	17.92676	1
TACTTAC	590	0.0	17.559322	31
CTAGACA	300	0.0	15.416667	4
TATACCG	120	1.9379695E-6	15.416666	5
GTCTTAT	275	0.0	14.799999	1
TAACACG	105	1.6570538E-4	14.095238	4
GTACTTA	760	0.0	13.631579	30
ACTAGAC	250	1.0913936E-11	13.32	3
CTCTTAT	6630	0.0	13.309955	37
CGCGCTA	85	0.009409411	13.058824	36
GTACTAA	85	0.009409411	13.058824	1
GTATCAA	2470	0.0	12.95749	2
GTAACGT	130	7.0052374E-5	12.807693	26
GGAGTGT	1270	0.0	12.673228	6
AGTGTAC	950	0.0	12.657895	27
GTATAAA	295	1.8189894E-12	12.542373	1
TCAATAC	345	0.0	12.333333	3
CGTTAGA	125	7.224742E-4	11.84	2
>>END_MODULE