FastQCFastQC Report
Thu 9 Feb 2017
SRR3211911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291121
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC27700.021.77256437
CTCGTTA8800.018.522
TCGTTAC8650.017.5375723
TACTTAC10300.017.2427231
CGTTACG7200.016.44444524
GTACTTA11800.015.52118730
AGTGTAC14500.015.18275927
TCTCGTT11050.014.73303221
TCTTATA46350.014.64832837
GTAACGT1406.0041566E-714.53571426
GTCTTAG4800.014.2604171
CGTATTA2101.364242E-1014.09523914
AGTATCG1203.3040327E-513.8750018
CGTAATA800.00630191113.8752
ATCTCGT12300.013.83739920
GATCTCG12150.013.70370419
TTATACT3800.013.6315794
GTGTACT15100.013.59933728
TTACATA5750.013.51304234
ACTTACA9000.013.36111232
Produced by FastQC (version 0.11.5)