##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211911.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3291121
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.609125583653714	34.0	31.0	34.0	31.0	34.0
2	32.73571315062558	34.0	31.0	34.0	31.0	34.0
3	32.81438087508785	34.0	31.0	34.0	31.0	34.0
4	36.258050372502254	37.0	37.0	37.0	35.0	37.0
5	36.16413799431865	37.0	35.0	37.0	35.0	37.0
6	36.18361859074765	37.0	36.0	37.0	35.0	37.0
7	36.170375078886494	37.0	36.0	37.0	35.0	37.0
8	36.153353523009336	37.0	36.0	37.0	35.0	37.0
9	38.01062647043363	39.0	38.0	39.0	35.0	39.0
10	37.89425973703185	39.0	38.0	39.0	35.0	39.0
11	37.991992394080924	39.0	38.0	39.0	35.0	39.0
12	37.932950505314146	39.0	38.0	39.0	35.0	39.0
13	37.97973122227958	39.0	38.0	39.0	35.0	39.0
14	39.393419141988396	40.0	39.0	41.0	37.0	41.0
15	39.366870437155	40.0	39.0	41.0	36.0	41.0
16	39.32936376389686	40.0	39.0	41.0	36.0	41.0
17	39.31520445465238	40.0	39.0	41.0	36.0	41.0
18	39.29942290180154	40.0	39.0	41.0	36.0	41.0
19	39.31683733293306	40.0	39.0	41.0	36.0	41.0
20	39.27288543933815	40.0	39.0	41.0	36.0	41.0
21	39.26354181447598	40.0	39.0	41.0	36.0	41.0
22	39.24124424474214	40.0	39.0	41.0	36.0	41.0
23	39.18713441407958	40.0	39.0	41.0	36.0	41.0
24	39.162628174412305	40.0	39.0	41.0	36.0	41.0
25	39.11169902291651	40.0	39.0	41.0	36.0	41.0
26	39.02929579313553	40.0	39.0	41.0	36.0	41.0
27	38.955278459831774	40.0	39.0	41.0	35.0	41.0
28	38.893240631383655	40.0	38.0	41.0	35.0	41.0
29	38.84282103271195	40.0	38.0	41.0	35.0	41.0
30	38.801175040358586	40.0	38.0	41.0	35.0	41.0
31	38.755547122090015	40.0	38.0	41.0	35.0	41.0
32	38.69919033666644	40.0	38.0	41.0	35.0	41.0
33	38.665785305371635	40.0	38.0	41.0	35.0	41.0
34	38.62213057496215	40.0	38.0	41.0	35.0	41.0
35	38.58325050947686	40.0	38.0	41.0	35.0	41.0
36	38.5147941993017	40.0	38.0	41.0	35.0	41.0
37	38.502058113329774	40.0	38.0	41.0	35.0	41.0
38	38.45759332458454	40.0	38.0	41.0	34.0	41.0
39	38.41337313334879	40.0	38.0	41.0	34.0	41.0
40	38.375698736084146	40.0	38.0	41.0	34.0	41.0
41	38.30361174809434	40.0	38.0	41.0	34.0	41.0
42	38.23246547301056	40.0	38.0	41.0	34.0	41.0
43	37.31418231052581	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	0.0
14	2.0
15	4.0
16	3.0
17	18.0
18	37.0
19	81.0
20	180.0
21	402.0
22	851.0
23	1539.0
24	2591.0
25	3980.0
26	6094.0
27	8789.0
28	12692.0
29	18089.0
30	25117.0
31	33956.0
32	45792.0
33	61977.0
34	88225.0
35	126753.0
36	192755.0
37	330428.0
38	806055.0
39	1524708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18792897617559	16.727978096217065	12.312370162020784	27.77172276558656
2	18.461460396017042	19.180090917350046	35.86635070542833	26.492097981204584
3	20.037731824505997	20.947057248882675	29.31177553180208	29.703435394809247
4	15.492502402676777	14.441948503260743	33.187081240707954	36.87846785335452
5	16.309427699558903	34.805405209957335	33.250220821416164	15.63494626906759
6	37.08429437872385	32.91963437381974	14.651390817900648	15.344680429555765
7	30.950183843134297	29.62589342658626	19.920841561279577	19.503081168999863
8	27.636966249493717	34.852744703096604	18.745071967879635	18.765217079530043
9	28.52474886216581	14.132631404314822	17.276879215319035	40.06574051820033
10	17.040698290947066	25.797228360792566	32.277938125034	24.884135223226373
11	38.29692071485673	20.46120455613756	20.076958580374285	21.164916148631423
12	22.596464851945584	23.032668807983665	29.133173772705412	25.237692567365343
13	32.13822888918396	18.64929912938479	22.98101467554672	26.231457305884533
14	23.406887805097412	18.78095639753142	24.114306341213222	33.69784945615795
15	26.94440587264947	25.696229339486454	21.071027166731334	26.28833762113274
16	25.722907179650946	24.886292542875207	23.30278953584508	26.088010741628764
17	25.65730643145603	24.34690186109839	23.760840151425608	26.23495155601997
18	25.664781088267492	24.68274487628987	24.261034462117923	25.391439573324714
19	27.17879409477804	24.145329205459173	23.94302731500908	24.732849384753706
20	26.257679374292227	23.574672581166116	23.901400161221662	26.26624788332
21	25.799811067414414	24.339913360827513	24.497002693003385	25.363272878754685
22	26.7068880177909	23.50955798951178	23.28395096989749	26.49960302279983
23	25.836819734066296	23.82094125375518	24.127402183025175	26.21483682915335
24	25.553056238284768	23.455229996101632	24.343316456611593	26.648397309002007
25	26.27785487072642	23.240014572542304	23.844580615541027	26.63754994119025
26	25.739983428138924	23.617120124115765	24.40958567004981	26.2333107776955
27	26.58003154548253	24.081794622561738	23.719000304151685	25.61917352780405
28	25.816097311523944	24.818959862004466	23.115163495963838	26.24977933050775
29	25.872673778934292	24.5686500131718	23.926680301331977	25.631995906561926
30	26.860270406344828	23.969249383416773	23.454288067804253	25.716192142434142
31	26.12471556044278	23.67515506114786	24.008050752312055	26.19207862609731
32	25.479190828899938	24.070278789506673	24.00416149998739	26.44636888160599
33	25.623761630155805	23.443653393479	24.14040687048577	26.792178105879426
34	26.027514637109974	23.637386774901316	24.85417582641295	25.48092276157577
35	25.900172008261013	23.398835837394007	24.424626138024095	26.276366016320885
36	25.476729661413238	23.008452135305873	25.234897167256992	26.279921036023897
37	24.99437121880356	22.759539986527386	25.58225601550353	26.66383277916552
38	25.50586866906443	22.240142492482047	25.473296180845374	26.780692657608153
39	24.38974440623727	22.416830010200172	26.77935572712155	26.414069856441014
40	25.446010645005153	21.830099835284088	26.71469690722401	26.009192612486746
41	24.335355643259547	22.216077743723186	27.08195778885067	26.366608824166597
42	23.792349172212145	22.296718959892388	27.465596068938215	26.445335798957252
43	23.284072509032637	21.987371476162682	27.21367582656487	27.51488018823981
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	46.0
2	54.0
3	150.0
4	246.0
5	246.0
6	311.5
7	377.0
8	365.5
9	354.0
10	465.5
11	577.0
12	577.0
13	1003.0
14	1429.0
15	2237.0
16	3045.0
17	3149.0
18	3253.0
19	3253.0
20	4019.0
21	4785.0
22	5697.5
23	6610.0
24	9118.5
25	11627.0
26	11627.0
27	15474.5
28	19322.0
29	23929.5
30	28537.0
31	35884.5
32	43232.0
33	43232.0
34	52854.0
35	62476.0
36	74770.5
37	87065.0
38	102707.5
39	118350.0
40	118350.0
41	137224.5
42	156099.0
43	177826.5
44	199554.0
45	227118.0
46	254682.0
47	254682.0
48	281982.5
49	309283.0
50	327187.5
51	345092.0
52	345224.5
53	345357.0
54	345357.0
55	325051.5
56	304746.0
57	283982.0
58	263218.0
59	243051.0
60	222884.0
61	222884.0
62	195498.5
63	168113.0
64	141894.0
65	115675.0
66	96955.5
67	78236.0
68	78236.0
69	64332.0
70	50428.0
71	42036.0
72	33644.0
73	27789.0
74	21934.0
75	21934.0
76	17825.5
77	13717.0
78	10867.0
79	8017.0
80	6317.5
81	4618.0
82	4618.0
83	3548.0
84	2478.0
85	1881.5
86	1285.0
87	885.0
88	485.0
89	485.0
90	314.5
91	144.0
92	91.0
93	38.0
94	26.0
95	14.0
96	14.0
97	8.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3291121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.4500491171343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98465511159148	47.33557069006702
2	9.991900561127535	11.680541571428522
3	3.2803627648625566	5.752120941847047
4	1.6441798367145015	3.8440956885345794
5	0.9181854873968581	2.683399341849312
6	0.631622145048493	2.215100724093248
7	0.43706851675187747	1.7882673390190194
8	0.3257070340816763	1.523007371189609
9	0.2512568447966684	1.321737742744331
>10	1.426475977813683	14.936989400163798
>50	0.07582701612688292	3.001989662534435
>100	0.031198562341563863	3.202186481015471
>500	0.0013001177890311637	0.5160461244857697
>1k	2.600235571823085E-4	0.1989469210279354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0384783786436293E-5	0.0
5	0.0	0.0	0.0	3.0384783786436293E-5	0.0
6	0.0	0.0	0.0	3.0384783786436293E-5	6.0769567572872585E-5
7	0.0	0.0	0.0	3.0384783786436293E-5	6.0769567572872585E-5
8	0.0	0.0	0.0	6.0769567572872585E-5	6.0769567572872585E-5
9	0.0	0.0	0.0	9.115435135930888E-5	6.0769567572872585E-5
10	0.0	3.0384783786436293E-5	0.0	9.115435135930888E-5	6.0769567572872585E-5
11	0.0	3.0384783786436293E-5	0.0	9.115435135930888E-5	6.0769567572872585E-5
12	0.0	3.0384783786436293E-5	0.0	9.115435135930888E-5	6.0769567572872585E-5
13	0.0	3.0384783786436293E-5	0.0	9.115435135930888E-5	9.115435135930888E-5
14	0.0	3.0384783786436293E-5	0.0	9.115435135930888E-5	1.2153913514574517E-4
15	0.0	6.0769567572872585E-5	0.0	1.5192391893218148E-4	1.8230870271861776E-4
16	0.0	6.0769567572872585E-5	0.0	1.8230870271861776E-4	2.1269348650505406E-4
17	0.0	6.0769567572872585E-5	0.0	2.7346305407792665E-4	2.1269348650505406E-4
18	0.0	6.0769567572872585E-5	0.0	3.0384783786436295E-4	2.1269348650505406E-4
19	0.0	6.0769567572872585E-5	3.0384783786436293E-5	3.950021892236718E-4	2.1269348650505406E-4
20	0.0	6.0769567572872585E-5	3.0384783786436293E-5	5.16541324369417E-4	2.7346305407792665E-4
21	0.0	6.0769567572872585E-5	3.0384783786436293E-5	6.380804595151622E-4	2.7346305407792665E-4
22	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.0011546217838845792	3.342326216507992E-4
23	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.0013977000541760694	4.253869730101081E-4
24	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.0020965500812641044	4.253869730101081E-4
25	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.002765015324565703	4.253869730101081E-4
26	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.0043146392976739535	5.16541324369417E-4
27	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.01680278543389927	5.16541324369417E-4
28	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.06025302624850317	5.469261081558533E-4
29	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.13107995725468616	5.773108919422896E-4
30	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.2283112653712823	5.773108919422896E-4
31	0.0	6.0769567572872585E-5	3.0384783786436293E-5	0.6804368481134544	5.773108919422896E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2770	0.0	21.772564	37
CTCGTTA	880	0.0	18.5	22
TCGTTAC	865	0.0	17.53757	23
TACTTAC	1030	0.0	17.24272	31
CGTTACG	720	0.0	16.444445	24
GTACTTA	1180	0.0	15.521187	30
AGTGTAC	1450	0.0	15.182759	27
TCTCGTT	1105	0.0	14.733032	21
TCTTATA	4635	0.0	14.648328	37
GTAACGT	140	6.0041566E-7	14.535714	26
GTCTTAG	480	0.0	14.260417	1
CGTATTA	210	1.364242E-10	14.095239	14
AGTATCG	120	3.3040327E-5	13.875001	8
CGTAATA	80	0.006301911	13.875	2
ATCTCGT	1230	0.0	13.837399	20
GATCTCG	1215	0.0	13.703704	19
TTATACT	380	0.0	13.631579	4
GTGTACT	1510	0.0	13.599337	28
TTACATA	575	0.0	13.513042	34
ACTTACA	900	0.0	13.361112	32
>>END_MODULE