FastQCFastQC Report
Thu 9 Feb 2017
SRR3211910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3211910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614109
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.12151858571178098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC38850.018.57142837
TCTTATA68400.016.36330437
GCGGTAA4650.015.91397923
CGCGGTA4750.015.18947322
TATACTG5650.014.0796455
ACTCCGT7400.013.756
GTAACGT4200.013.65476126
GTATTAG5850.013.5982911
CTCTTAT107200.013.27098837
CGGTAAT5600.013.21428524
TTATACT6600.013.1742434
TAAACCG1857.399103E-813.05
TAGTACT4000.012.9500014
TATCGCG6750.012.8814827
TAATACT5900.012.5423734
TCGCGCG6800.012.5147069
GTATCGC8150.012.4846646
GTACTAG4450.012.47190951
TTAGCCG5200.012.4519234
ATTAGAC3150.012.3333343
Produced by FastQC (version 0.11.5)