##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211908.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5032089
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.624246510743355	34.0	31.0	34.0	31.0	34.0
2	32.749821197518564	34.0	31.0	34.0	31.0	34.0
3	32.82941398691478	34.0	31.0	34.0	31.0	34.0
4	36.26777149609238	37.0	37.0	37.0	35.0	37.0
5	36.180716398299	37.0	35.0	37.0	35.0	37.0
6	36.19813004102272	37.0	37.0	37.0	35.0	37.0
7	36.18365493933037	37.0	36.0	37.0	35.0	37.0
8	36.162304760508015	37.0	36.0	37.0	35.0	37.0
9	38.02030111152644	39.0	38.0	39.0	35.0	39.0
10	37.90673972578784	39.0	38.0	39.0	35.0	39.0
11	37.9978466199624	39.0	38.0	39.0	35.0	39.0
12	37.94125362250151	39.0	38.0	39.0	35.0	39.0
13	37.98889407560161	39.0	38.0	39.0	35.0	39.0
14	39.41130095274547	40.0	39.0	41.0	37.0	41.0
15	39.38538785780617	40.0	39.0	41.0	36.0	41.0
16	39.34975235930843	40.0	39.0	41.0	36.0	41.0
17	39.3377943037176	40.0	39.0	41.0	36.0	41.0
18	39.324123043133774	40.0	39.0	41.0	36.0	41.0
19	39.33363618966199	40.0	39.0	41.0	36.0	41.0
20	39.29617918125057	40.0	39.0	41.0	36.0	41.0
21	39.284895199588085	40.0	39.0	41.0	36.0	41.0
22	39.26272289699169	40.0	39.0	41.0	36.0	41.0
23	39.21052211119478	40.0	39.0	41.0	36.0	41.0
24	39.1867681593072	40.0	39.0	41.0	36.0	41.0
25	39.13820800864214	40.0	39.0	41.0	36.0	41.0
26	39.05575020632584	40.0	39.0	41.0	36.0	41.0
27	38.984155685640694	40.0	39.0	41.0	35.0	41.0
28	38.92978721163318	40.0	39.0	41.0	35.0	41.0
29	38.87824022985285	40.0	38.0	41.0	35.0	41.0
30	38.83370266304908	40.0	38.0	41.0	35.0	41.0
31	38.792792019378034	40.0	38.0	41.0	35.0	41.0
32	38.73898752585656	40.0	38.0	41.0	35.0	41.0
33	38.70728240299407	40.0	38.0	41.0	35.0	41.0
34	38.66091239642224	40.0	38.0	41.0	35.0	41.0
35	38.618690368950155	40.0	38.0	41.0	35.0	41.0
36	38.56233981553188	40.0	38.0	41.0	35.0	41.0
37	38.54279008181294	40.0	38.0	41.0	35.0	41.0
38	38.49378101221978	40.0	38.0	41.0	35.0	41.0
39	38.45214184407311	40.0	38.0	41.0	34.0	41.0
40	38.410006857986815	40.0	38.0	41.0	34.0	41.0
41	38.33813690497128	40.0	38.0	41.0	34.0	41.0
42	38.259972349455666	40.0	38.0	41.0	34.0	41.0
43	37.3763017704973	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	3.0
13	3.0
14	4.0
15	6.0
16	5.0
17	14.0
18	76.0
19	98.0
20	287.0
21	595.0
22	1264.0
23	2367.0
24	3912.0
25	6154.0
26	9555.0
27	13939.0
28	20018.0
29	27800.0
30	38922.0
31	51915.0
32	69127.0
33	94065.0
34	130675.0
35	188868.0
36	285376.0
37	490159.0
38	1198014.0
39	2398864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18103276790215	17.10738820398447	12.370289953138746	27.34128907497463
2	18.12406338600132	19.35498358633959	36.184356039807724	26.33659698785137
3	19.876178660592053	21.268224786962232	29.297891988794316	29.5577045636514
4	15.004225879152774	14.59701924985826	33.73378332537441	36.664971545614556
5	16.028532086773506	35.004527940582925	33.563873770913034	15.403066201730534
6	36.70239139252108	33.4817806282838	14.79113346365694	15.024694515538178
7	30.836875102964196	29.68133115292675	20.196542628717417	19.285251115391638
8	27.47117548994066	34.962934876549284	18.91057570722616	18.655313926283895
9	27.97281606108318	14.334364912862233	17.63609506906575	40.05672395698884
10	16.932391299120507	25.947573661753598	32.51927777906949	24.60075726005641
11	37.91051787836026	20.695579907271117	20.411642162926768	20.982260051441855
12	22.429333026502512	23.297401933868816	29.50603616112513	24.767228878503538
13	31.847230841902835	18.820116257880176	23.384542681975617	25.94811021824137
14	23.154240713946038	18.891716740304076	24.458708898034196	33.49533364771569
15	26.426738477797194	26.07000790327834	21.297278327152004	26.205975291772464
16	25.684621237819922	25.002518834623157	23.388437684627597	25.924422242929328
17	25.346272691122913	24.80017742134529	24.102018068440366	25.751531819091433
18	25.50119840885167	24.68581139959965	24.580348241058534	25.23264195049014
19	26.756621355464898	24.384703847646573	24.22926144589255	24.629413350995975
20	25.995207954390313	24.001582642914304	24.217556565474098	25.785652837221278
21	25.57254054926294	24.52905741531996	24.617390511177366	25.281011524239734
22	26.24236177062846	23.905737756228078	23.69661983323427	26.15528063990919
23	25.649069402389348	24.10533676967955	24.33152513796954	25.914068689961567
24	25.486453041669176	23.879168273852073	24.35672342043235	26.277655264046402
25	26.020684451328265	23.705403461663735	24.143213683223806	26.13069840378419
26	25.620592163612372	23.954087457515158	24.51403383366232	25.91128654521015
27	26.191309414440006	24.34885392527835	24.090651019884586	25.369185640397056
28	25.680090316367615	24.716156649852575	23.756038496139475	25.847714537640332
29	25.729413768317688	24.685413950349446	24.17916296790458	25.406009313428278
30	26.255000656784887	24.317833011300078	23.86122344020545	25.565942891709586
31	25.869772971026546	23.899040736362174	24.223736901314744	26.00744939129654
32	25.228766820300674	24.357856150795428	24.24311255226209	26.170264476641808
33	25.358712852654236	23.873266152486572	24.43198043595811	26.336040558901086
34	25.74869005695249	23.88874680078194	24.941987313817382	25.420575828448182
35	25.6153458335097	23.940057498983027	24.604115706220618	25.840480961286655
36	25.331447834090376	23.57237719762111	25.075927711135478	26.020247257153045
37	25.086082539478138	23.244958505304655	25.35225430233845	26.31670465287875
38	25.21471698930603	22.958814917621687	25.469322978985463	26.357145114086816
39	24.4982550984293	22.929960102057017	26.43210404267492	26.13968075683876
40	25.19893825407301	22.611742359882744	26.512031086890552	25.677288299153687
41	24.429714975231956	22.640875389922556	26.84578909474773	26.083620540097762
42	23.896715658248493	22.90482143698174	27.108443431743755	26.090019473026015
43	23.611088754590785	22.54526897278645	26.945111662373222	26.898530610249537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	81.0
2	97.0
3	299.5
4	502.0
5	502.0
6	666.5
7	831.0
8	783.5
9	736.0
10	1026.5
11	1317.0
12	1317.0
13	2222.0
14	3127.0
15	5089.5
16	7052.0
17	7038.0
18	7024.0
19	7024.0
20	8824.0
21	10624.0
22	12283.5
23	13943.0
24	18537.0
25	23131.0
26	23131.0
27	29835.5
28	36540.0
29	44540.0
30	52540.0
31	64448.0
32	76356.0
33	76356.0
34	91990.5
35	107625.0
36	126580.5
37	145536.0
38	170319.5
39	195103.0
40	195103.0
41	222412.0
42	249721.0
43	280732.5
44	311744.0
45	349310.0
46	386876.0
47	386876.0
48	423126.5
49	459377.0
50	481489.5
51	503602.0
52	505675.5
53	507749.0
54	507749.0
55	484430.5
56	461112.0
57	431167.0
58	401222.0
59	369458.0
60	337694.0
61	337694.0
62	296043.5
63	254393.0
64	214223.0
65	174053.0
66	143953.5
67	113854.0
68	113854.0
69	93358.5
70	72863.0
71	59155.5
72	45448.0
73	36985.5
74	28523.0
75	28523.0
76	23093.5
77	17664.0
78	14330.0
79	10996.0
80	8773.5
81	6551.0
82	6551.0
83	5097.0
84	3643.0
85	2712.0
86	1781.0
87	1278.0
88	775.0
89	775.0
90	496.5
91	218.0
92	136.5
93	55.0
94	38.5
95	22.0
96	22.0
97	12.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5032089.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.438961715924286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30339844988177	43.17194672155802
2	10.64086443308507	11.58555222994117
3	3.627364746081015	5.924099116247933
4	1.7395669685379207	3.7880087841008927
5	1.0441216033236551	2.8420447995052966
6	0.6819367407257937	2.22743568726322
7	0.5131846276029043	1.9556066806692758
8	0.3729222187501255	1.6241198711644498
9	0.269888041039943	1.3223182260383335
>10	1.656796563109375	16.629612786507778
>50	0.10579494644226796	3.9025100333321467
>100	0.042074063330026015	4.025659506980047
>500	0.0015008875379571777	0.5381283978903046
>1k	5.857105521802791E-4	0.46295715880107097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9872462510102664E-5
2	0.0	0.0	0.0	0.0	3.974492502020533E-5
3	0.0	0.0	0.0	1.9872462510102664E-5	3.974492502020533E-5
4	0.0	0.0	0.0	3.974492502020533E-5	5.961738753030799E-5
5	0.0	0.0	0.0	3.974492502020533E-5	5.961738753030799E-5
6	0.0	0.0	0.0	3.974492502020533E-5	1.1923477506061598E-4
7	0.0	0.0	0.0	3.974492502020533E-5	1.1923477506061598E-4
8	0.0	0.0	0.0	3.974492502020533E-5	1.3910723757071865E-4
9	0.0	0.0	1.9872462510102664E-5	3.974492502020533E-5	1.5897970008082131E-4
10	0.0	0.0	1.9872462510102664E-5	3.974492502020533E-5	1.9872462510102664E-4
11	0.0	0.0	1.9872462510102664E-5	5.961738753030799E-5	2.185970876111293E-4
12	0.0	0.0	1.9872462510102664E-5	5.961738753030799E-5	2.9808693765153994E-4
13	0.0	0.0	1.9872462510102664E-5	5.961738753030799E-5	2.9808693765153994E-4
14	0.0	0.0	1.9872462510102664E-5	7.948985004041066E-5	3.775767876919506E-4
15	0.0	1.9872462510102664E-5	1.9872462510102664E-5	9.936231255051332E-5	5.166840252626692E-4
16	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	9.936231255051332E-5	6.160463378131826E-4
17	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	1.1923477506061598E-4	6.160463378131826E-4
18	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	1.3910723757071865E-4	6.756637253434905E-4
19	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	2.3846955012123196E-4	6.756637253434905E-4
20	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	3.775767876919506E-4	8.147709629142092E-4
21	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	6.756637253434905E-4	8.346434254243118E-4
22	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.0010134955880152358	8.545158879344145E-4
23	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.0012718376006465705	9.340057379748251E-4
24	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.0018083940884193424	9.538782004849278E-4
25	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.0025039302762729356	9.737506629950305E-4
26	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.004073854814571046	9.93623125505133E-4
27	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.013811361444521351	9.93623125505133E-4
28	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.05119146342602446	9.93623125505133E-4
29	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.10832479314256961	9.93623125505133E-4
30	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.18632420849472256	9.93623125505133E-4
31	1.9872462510102664E-5	1.9872462510102664E-5	1.9872462510102664E-5	0.5510037680176165	0.0010333680505253385
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3480	0.0	19.775864	37
TCTTATA	5945	0.0	15.963835	37
TACTTAC	1085	0.0	14.152074	31
TATACCG	265	0.0	13.962264	5
TCTATAC	540	0.0	13.018518	3
TAGCGTA	115	3.580909E-4	12.869565	14
CTCTTAT	9295	0.0	12.47929	37
CGCCGTT	245	1.5661499E-9	12.081633	25
GGACCGT	585	0.0	12.017094	6
TATACTG	655	0.0	11.862595	5
CGCGGTA	360	0.0	11.819445	22
GTATATG	580	0.0	11.801724	1
GGTATCA	5180	0.0	11.607142	1
GTAACGT	320	5.456968E-12	11.562501	26
ACTATCG	465	0.0	11.537634	11
TAATCGA	295	2.1100277E-10	11.288136	20
GTACCGT	495	0.0	11.212121	6
ACCGTCC	595	0.0	11.193277	8
AGTGTAC	1555	0.0	11.064308	27
GTATAGG	670	0.0	11.044776	1
>>END_MODULE