##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3211907.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5432091
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.577742530454664	34.0	31.0	34.0	31.0	34.0
2	32.71035499957567	34.0	31.0	34.0	31.0	34.0
3	32.78932256473612	34.0	31.0	34.0	31.0	34.0
4	36.241959127709755	37.0	37.0	37.0	35.0	37.0
5	36.14585322668564	37.0	35.0	37.0	35.0	37.0
6	36.16203318390653	37.0	36.0	37.0	35.0	37.0
7	36.14730386512303	37.0	36.0	37.0	35.0	37.0
8	36.128218581021564	37.0	36.0	37.0	35.0	37.0
9	37.977190367392595	39.0	38.0	39.0	35.0	39.0
10	37.86214001201379	39.0	38.0	39.0	35.0	39.0
11	37.954597594186104	39.0	38.0	39.0	35.0	39.0
12	37.894201146483006	39.0	38.0	39.0	35.0	39.0
13	37.940033037001776	39.0	38.0	39.0	35.0	39.0
14	39.35750045424497	40.0	39.0	41.0	36.0	41.0
15	39.32526406497976	40.0	39.0	41.0	36.0	41.0
16	39.29371856988405	40.0	39.0	41.0	36.0	41.0
17	39.28000985255954	40.0	39.0	41.0	36.0	41.0
18	39.2645693895776	40.0	39.0	41.0	36.0	41.0
19	39.27091298728243	40.0	39.0	41.0	36.0	41.0
20	39.23129675110376	40.0	39.0	41.0	36.0	41.0
21	39.2215307144155	40.0	39.0	41.0	36.0	41.0
22	39.19458436907629	40.0	39.0	41.0	36.0	41.0
23	39.140405416625015	40.0	39.0	41.0	36.0	41.0
24	39.11110785883373	40.0	39.0	41.0	36.0	41.0
25	39.06416589854625	40.0	39.0	41.0	36.0	41.0
26	38.977495590556195	40.0	39.0	41.0	36.0	41.0
27	38.902816429253484	40.0	38.0	41.0	35.0	41.0
28	38.84947822118591	40.0	38.0	41.0	35.0	41.0
29	38.79439777426409	40.0	38.0	41.0	35.0	41.0
30	38.74748121855838	40.0	38.0	41.0	35.0	41.0
31	38.70653455547781	40.0	38.0	41.0	35.0	41.0
32	38.65158720647353	40.0	38.0	41.0	35.0	41.0
33	38.618983739410844	40.0	38.0	41.0	35.0	41.0
34	38.57230521359086	40.0	38.0	41.0	35.0	41.0
35	38.527030382959346	40.0	38.0	41.0	35.0	41.0
36	38.47506659958384	40.0	38.0	41.0	34.0	41.0
37	38.44882035297273	40.0	38.0	41.0	34.0	41.0
38	38.39711540178543	40.0	38.0	41.0	34.0	41.0
39	38.36032864692436	40.0	38.0	41.0	34.0	41.0
40	38.313415220768576	40.0	38.0	41.0	34.0	41.0
41	38.24758366529574	40.0	38.0	41.0	34.0	41.0
42	38.16653126760947	40.0	38.0	41.0	34.0	41.0
43	37.264866328638455	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	1.0
15	7.0
16	8.0
17	33.0
18	70.0
19	187.0
20	341.0
21	722.0
22	1514.0
23	2628.0
24	4577.0
25	7291.0
26	11038.0
27	16171.0
28	23252.0
29	32314.0
30	44599.0
31	59252.0
32	79460.0
33	105784.0
34	148003.0
35	211216.0
36	320463.0
37	548485.0
38	1322130.0
39	2492541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11746618383234	17.04122777030061	12.293479619542456	27.547826426324594
2	18.444389094365317	19.196401533037648	35.75711452551145	26.60209484708559
3	19.94968420079855	21.026359830864394	29.14023347547013	29.883722492866927
4	15.157275531650702	14.591084722255204	33.45389464204484	36.79774510404925
5	16.196138834934835	34.709083481848886	33.460945333942306	15.633832349273971
6	36.94774259120475	33.305204202212366	14.719064905208695	15.027988301374185
7	31.069067141916435	29.282627997211385	20.12374240416812	19.524562456704057
8	27.56861031967248	34.56862191741633	18.796629143363024	19.066138619548163
9	27.815826354897222	14.188274828238335	17.67617663253432	40.31972218433012
10	17.048057552791366	25.770426158177393	32.49847986714508	24.683036421886158
11	38.08321694168967	20.471416992093836	20.388373464288428	21.05699260192806
12	22.56447839331116	23.299020579736236	29.334449662201905	24.802051364750703
13	31.886082173512925	18.787553448570726	23.187056328769163	26.139308049147186
14	23.32315493241921	18.839080567685627	24.238198513242875	33.59956598665229
15	26.392930457166496	25.95160868991333	21.232946944371882	26.42251390854829
16	25.865582885117348	24.847798021056715	23.13521625466142	26.15140283916451
17	25.417689063014592	24.67386868150773	24.046633239391607	25.861809016086067
18	25.694617413441712	24.37074415726835	24.439262891582633	25.495375537707304
19	26.789352387506028	24.18729730411365	24.128848356921857	24.894501951458473
20	26.22146793932576	23.882147776979433	24.105008550114494	25.791375733580306
21	25.828654195962475	24.25038534884633	24.347125259867703	25.573835195323497
22	26.390666135747725	23.83595930186	23.726259372311695	26.04711519008058
23	25.920368417981216	23.906742357593053	24.14473542508769	26.028153799338043
24	25.74601566873603	23.779443311976916	24.093466033613943	26.381074985673102
25	26.150261473896514	23.63395605854173	24.030617307405198	26.185165160156558
26	25.832869883807174	23.92881488914674	24.230687593414764	26.00762763363132
27	26.215466567110163	24.161450903528678	23.993486117960835	25.629596411400325
28	25.902124982810488	24.42908264975679	23.715085038155657	25.95370732927707
29	25.835042159639816	24.474774078711125	23.975647683369075	25.714536078279988
30	26.212521108354036	24.231166230462634	23.835462255694907	25.72085040548842
31	25.91834341508638	23.75142463555931	24.11684561249066	26.213386336863646
32	25.31851914851942	24.15826612624862	24.257914677791664	26.26530004744029
33	25.5430367422048	23.795072652501588	24.358391639609867	26.303498965683747
34	25.84544331087237	23.679868396902776	24.796878402810265	25.677809889414593
35	25.714186305052696	23.722982549445508	24.565788754275285	25.99704239122651
36	25.40949700584913	23.27560418262507	25.07358584383067	26.24131296769513
37	25.26007020132763	22.925867773570065	25.298784574853407	26.515277450248902
38	25.105396798396782	22.665746947170067	25.68966536090798	26.539190893525166
39	24.62640997729972	22.48095622845788	26.487350819417422	26.405282974824978
40	25.019186902428547	22.277682756051032	26.737862086625576	25.965268254894845
41	24.25487717344941	22.32455236850782	27.12371718367752	26.29685327436525
42	23.736439614137538	22.527954704735247	27.369386853055293	26.366218828071915
43	23.456878759947138	22.219546763852076	27.284410367941188	27.0391641082596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	75.5
2	97.0
3	276.5
4	456.0
5	456.0
6	627.5
7	799.0
8	733.5
9	668.0
10	991.5
11	1315.0
12	1315.0
13	2214.0
14	3113.0
15	4852.5
16	6592.0
17	6685.0
18	6778.0
19	6778.0
20	8575.0
21	10372.0
22	12180.0
23	13988.0
24	18756.0
25	23524.0
26	23524.0
27	30466.0
28	37408.0
29	46043.5
30	54679.0
31	67615.5
32	80552.0
33	80552.0
34	97286.0
35	114020.0
36	134864.5
37	155709.0
38	181366.0
39	207023.0
40	207023.0
41	237754.0
42	268485.0
43	301618.0
44	334751.0
45	374220.0
46	413689.0
47	413689.0
48	449775.0
49	485861.0
50	508650.5
51	531440.0
52	533546.0
53	535652.0
54	535652.0
55	516743.5
56	497835.0
57	469402.0
58	440969.0
59	406339.0
60	371709.0
61	371709.0
62	326266.0
63	280823.0
64	237554.5
65	194286.0
66	162284.5
67	130283.0
68	130283.0
69	107172.5
70	84062.0
71	69262.0
72	54462.0
73	45100.5
74	35739.0
75	35739.0
76	29150.5
77	22562.0
78	18681.0
79	14800.0
80	11697.0
81	8594.0
82	8594.0
83	6667.0
84	4740.0
85	3691.5
86	2643.0
87	1866.0
88	1089.0
89	1089.0
90	704.5
91	320.0
92	213.0
93	106.0
94	67.5
95	29.0
96	29.0
97	19.0
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5432091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.70237824521978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91288597247177	45.31250687078212
2	10.4998345377537	11.907311789438655
3	3.5450419489342084	6.030369284509159
4	1.7161565866946535	3.892406396271422
5	1.015876956868268	2.880131972947367
6	0.6398818026027754	2.1769692002049377
7	0.45726444842461667	1.8149587198865083
8	0.33378876699099197	1.5141293535943008
9	0.2474086607573576	1.2625793517066262
>10	1.5035089336090828	15.588971358986322
>50	0.09224277153608715	3.5484797247931374
>100	0.03490592614053179	3.5066783378291206
>500	0.0010726678852795025	0.41954772036915045
>1k	1.3001933078491326E-4	0.14495991868124786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.363646890304305E-5
2	0.0	0.0	0.0	0.0	9.204558612880381E-5
3	0.0	0.0	0.0	0.0	9.204558612880381E-5
4	0.0	0.0	0.0	3.681823445152152E-5	1.472729378060861E-4
5	0.0	0.0	0.0	3.681823445152152E-5	1.6568205503184684E-4
6	1.840911722576076E-5	0.0	0.0	3.681823445152152E-5	2.5772764116065063E-4
7	3.681823445152152E-5	0.0	0.0	3.681823445152152E-5	2.761367583864114E-4
8	5.5227351677282285E-5	0.0	0.0	3.681823445152152E-5	2.945458756121722E-4
9	5.5227351677282285E-5	0.0	0.0	5.5227351677282285E-5	3.4977322728945446E-4
10	5.5227351677282285E-5	0.0	0.0	1.1045470335456457E-4	3.6818234451521523E-4
11	5.5227351677282285E-5	0.0	0.0	1.6568205503184684E-4	3.6818234451521523E-4
12	5.5227351677282285E-5	0.0	0.0	1.6568205503184684E-4	3.86591461740976E-4
13	5.5227351677282285E-5	0.0	0.0	2.0250028948336836E-4	3.86591461740976E-4
14	5.5227351677282285E-5	0.0	0.0	2.0250028948336836E-4	4.6022793064401905E-4
15	5.5227351677282285E-5	0.0	0.0	2.2090940670912914E-4	4.970461650955405E-4
16	5.5227351677282285E-5	0.0	0.0	2.761367583864114E-4	5.338643995470621E-4
17	5.5227351677282285E-5	0.0	0.0	2.761367583864114E-4	5.338643995470621E-4
18	5.5227351677282285E-5	0.0	0.0	2.945458756121722E-4	5.890917512243444E-4
19	5.5227351677282285E-5	0.0	1.840911722576076E-5	3.1295499283793296E-4	5.890917512243444E-4
20	5.5227351677282285E-5	0.0	1.840911722576076E-5	3.6818234451521523E-4	6.443191029016266E-4
21	5.5227351677282285E-5	0.0	1.840911722576076E-5	5.338643995470621E-4	6.443191029016266E-4
22	5.5227351677282285E-5	0.0	1.840911722576076E-5	8.284102751592343E-4	6.627282201273874E-4
23	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.001086137916319885	8.284102751592343E-4
24	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.00169363878476999	8.46819392384995E-4
25	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.002245912301542813	8.652285096107558E-4
26	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.0039579602035385636	9.020467440622773E-4
27	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.016844342261571096	9.020467440622773E-4
28	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.05916690276359509	9.204558612880381E-4
29	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.12464813273562611	9.204558612880381E-4
30	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.21279098601256863	9.204558612880381E-4
31	5.5227351677282285E-5	0.0	1.840911722576076E-5	0.5968603986936154	9.388649785137988E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3870	0.0	20.45995	37
GGTATCA	3865	0.0	15.60414	1
TCTTATA	6710	0.0	15.549925	37
TATACCG	220	1.8189894E-12	15.136364	5
TCGTTAC	705	0.0	14.170213	23
CGTTACG	560	0.0	13.875001	24
CTCGTTA	775	0.0	13.367743	22
ATTATAC	380	0.0	13.144737	3
GTACTAG	410	0.0	13.085366	1
TATACTG	755	0.0	12.986754	5
TACGGAT	1315	0.0	12.9429655	27
TAGACAG	850	0.0	12.841177	5
CTCTTAT	10195	0.0	12.793036	37
TACACCG	320	0.0	12.71875	5
GTCTAGG	490	0.0	12.459184	1
TACTTAC	790	0.0	12.177215	31
GTTACGG	1140	0.0	11.846491	25
GTCTTAC	485	0.0	11.824742	1
TTACGGA	1460	0.0	11.657535	26
GTATCAA	5160	0.0	11.652132	2
>>END_MODULE