##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727619.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140191
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638319369298653	31.0	31.0	34.0	30.0	34.0
2	31.87834757509926	31.0	31.0	34.0	30.0	34.0
3	31.971874010582436	33.0	31.0	34.0	30.0	34.0
4	35.706398313966695	37.0	35.0	37.0	33.0	37.0
5	35.314523619288344	37.0	35.0	37.0	33.0	37.0
6	35.33105506007327	37.0	35.0	37.0	33.0	37.0
7	35.28750007674153	37.0	35.0	37.0	32.0	37.0
8	35.24710859847166	37.0	35.0	37.0	32.0	37.0
9	37.074182308051896	39.0	37.0	39.0	33.0	39.0
10	36.83199393785778	39.0	37.0	39.0	33.0	39.0
11	36.95333413436872	39.0	37.0	39.0	33.0	39.0
12	36.85024438887871	39.0	37.0	39.0	33.0	39.0
13	36.981067207160905	39.0	37.0	39.0	33.0	39.0
14	37.97271071250343	39.0	37.0	41.0	33.0	41.0
15	37.98154256611392	40.0	37.0	41.0	33.0	41.0
16	37.89663135386966	39.0	37.0	41.0	33.0	41.0
17	37.87325895398227	39.0	37.0	41.0	33.0	41.0
18	37.891207701165854	39.0	37.0	41.0	33.0	41.0
19	37.96882452150561	40.0	37.0	41.0	33.0	41.0
20	37.836809797656706	39.0	37.0	41.0	33.0	41.0
21	37.81714905660543	39.0	37.0	41.0	33.0	41.0
22	37.72437951185371	39.0	37.0	41.0	32.0	41.0
23	37.63792031335101	39.0	37.0	40.0	32.0	41.0
24	37.549464081018	39.0	36.0	41.0	32.0	41.0
25	37.50580648329973	39.0	36.0	41.0	32.0	41.0
26	37.349351994534246	39.0	36.0	40.0	32.0	41.0
27	37.14448193329012	39.0	36.0	40.0	31.0	41.0
28	37.011505966982725	39.0	36.0	40.0	31.0	41.0
29	36.92099218464275	39.0	36.0	40.0	31.0	41.0
30	36.863503570892945	39.0	35.0	40.0	31.0	41.0
31	36.75794230966566	39.0	35.0	40.0	31.0	41.0
32	36.611476498235824	38.0	35.0	40.0	30.0	41.0
33	36.56659191310929	38.0	35.0	40.0	30.0	41.0
34	36.531840717914804	38.0	35.0	40.0	30.0	41.0
35	36.41030757127534	38.0	35.0	40.0	30.0	41.0
36	36.29738263150647	38.0	35.0	40.0	30.0	41.0
37	36.16360767625775	38.0	35.0	40.0	30.0	41.0
38	36.08255371249203	38.0	35.0	40.0	30.0	41.0
39	35.92913204892864	38.0	35.0	40.0	29.0	41.0
40	35.86925699290733	38.0	35.0	40.0	29.0	41.0
41	35.794299376157156	38.0	35.0	40.0	28.0	41.0
42	35.69248047037733	38.0	35.0	40.0	27.0	41.0
43	34.447130349213424	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	1.0
13	0.0
14	0.0
15	1.0
16	7.0
17	10.0
18	40.0
19	81.0
20	181.0
21	390.0
22	698.0
23	1255.0
24	2140.0
25	3496.0
26	5611.0
27	8331.0
28	12398.0
29	17695.0
30	23906.0
31	32108.0
32	41611.0
33	54727.0
34	72949.0
35	97883.0
36	134312.0
37	189577.0
38	271347.0
39	169428.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.75335535888286	19.469545014826465	14.231299843622692	24.54579978266799
2	17.530659336900573	22.22285564436134	37.3479531061024	22.89853191263569
3	19.446917227026	24.210329672835513	32.33502106226062	24.00773203787786
4	14.559315062125556	16.905237806648184	35.340131609528584	33.19531552169768
5	14.006425239280084	37.21095851484532	34.69734456770839	14.085271678166203
6	32.359402942138644	35.3262742821159	16.41496907097144	15.899353704774025
7	26.30261070294363	32.813537381017746	22.780218402004575	18.10363351403405
8	25.26296032857653	36.377677073402616	21.031388600681815	17.32797399733904
9	26.921015864885796	15.345499131285898	21.157332411850298	36.57615259197801
10	16.58397584264391	28.032584014432672	33.862484443395886	21.520955699527537
11	33.15260337960921	23.46983970229549	22.526839801401696	20.850717116693605
12	20.22020871941631	26.31962539609592	31.687848790246548	21.772317094241227
13	29.81009322122346	21.440004350148353	25.799712504308488	22.950189924319698
14	20.867205582222628	22.719965339140547	28.63546546148847	27.777363617148353
15	24.689547628423657	28.644499035687883	25.00440715634486	21.661546179543603
16	21.50227461890157	27.558452925869435	28.28990932221005	22.64936313301894
17	21.964828699752935	27.19710995789302	27.659839447952137	23.178221894401904
18	21.5696317546797	26.77726801913013	29.088284331309406	22.56481589488077
19	23.69067989485972	26.08580492215778	29.900428963217568	20.323086219764935
20	22.460710530077854	25.70157105256926	29.691955119800106	22.14576329755278
21	22.019644077176544	26.82094491186126	30.683981894261574	20.47542911670062
22	22.60559853568393	25.83672384714491	29.00207070569755	22.555606911473607
23	21.428778160851998	26.691580621141547	29.87639790175506	22.0032433162514
24	21.099885896310354	25.93495300348801	30.366315819016286	22.598845281185344
25	21.639444619366405	26.296120562256675	29.547417932609537	22.517016885767386
26	21.217059247091058	26.296909903691574	30.237039232900454	22.248991616316914
27	22.156112440810354	27.437245163310358	29.092581856899415	21.314060538979874
28	21.036650876914482	27.778679186206524	28.31411579288032	22.87055414399868
29	21.21100762942349	27.799377472721677	29.96173448132813	21.027880416526703
30	23.10630411922213	26.554849143696103	28.625554841250285	21.71329189583149
31	21.728026269282953	26.66816349190618	30.022426067211544	21.581384171599318
32	21.09848262264831	27.493990042019277	29.314386800106295	22.093140535226116
33	21.124706299207766	26.034497728889285	29.619160298581555	23.2216356733214
34	21.55726540553293	26.69026505208338	30.52128985406831	21.23117968831538
35	21.539461370945745	26.42530944376863	29.498654172853495	22.536575012432127
36	21.18706427256486	26.30541725026772	30.14547562645206	22.362042850715362
37	20.505160977415187	26.117817102573166	30.893771306737207	22.483250613274443
38	21.359491523788556	25.400130329041364	30.44928437428466	22.79109377288542
39	19.873424715683598	25.905659665792836	31.92149385497693	22.299421763546633
40	21.371770168331448	25.127719829397005	31.451747996607587	22.048762005663963
41	20.164516295953923	25.984769218490587	31.40403669209808	22.44667779345741
42	19.84360515036516	26.357864603386627	31.128030303694732	22.670499942553484
43	19.118638894711502	26.01257157791984	30.688805647474855	24.179983879893808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3250.0
1	2781.5
2	2313.0
3	3485.5
4	4658.0
5	4658.0
6	5083.0
7	5508.0
8	4964.0
9	4420.0
10	5802.5
11	7185.0
12	7185.0
13	10792.0
14	14399.0
15	17072.0
16	19745.0
17	18031.5
18	16318.0
19	16318.0
20	18777.5
21	21237.0
22	17660.5
23	14084.0
24	15643.0
25	17202.0
26	17202.0
27	19749.0
28	22296.0
29	24902.0
30	27508.0
31	31397.5
32	35287.0
33	35287.0
34	38794.0
35	42301.0
36	45853.5
37	49406.0
38	53105.0
39	56804.0
40	56804.0
41	60553.0
42	64302.0
43	68473.5
44	72645.0
45	78463.5
46	84282.0
47	84282.0
48	89268.0
49	94254.0
50	96921.5
51	99589.0
52	95713.5
53	91838.0
54	91838.0
55	83181.5
56	74525.0
57	67477.5
58	60430.0
59	53903.5
60	47377.0
61	47377.0
62	40574.0
63	33771.0
64	27705.0
65	21639.0
66	17459.5
67	13280.0
68	13280.0
69	10499.0
70	7718.0
71	6088.0
72	4458.0
73	3519.5
74	2581.0
75	2581.0
76	2073.5
77	1566.0
78	1247.0
79	928.0
80	737.5
81	547.0
82	547.0
83	426.0
84	305.0
85	229.5
86	154.0
87	106.0
88	58.0
89	58.0
90	38.5
91	19.0
92	11.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1140191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.46320580141692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.19673161882022	57.95374318792567
2	7.098276215626948	9.435483859090334
3	2.1458256001630804	4.278553454327634
4	1.0388109649261204	2.7617082780261293
5	0.5827168871631763	1.9364616197743612
6	0.3852271608507212	1.5362059243150212
7	0.2862697939714709	1.3318485762018564
8	0.19939439718600058	1.06019126846581
9	0.13957420083408917	0.8348893951143929
>10	0.8483568879036467	10.17833472755804
>50	0.05671100519734943	2.543290299800495
>100	0.019855033386197353	2.277942713234909
>500	7.942002250544033E-4	0.3220770424874757
>1k	0.0010589336334058711	1.396429635455805
>5k	3.9710011252720164E-4	2.1528400182220757
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9643	0.8457354951933492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8417	0.7382096508392015	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6411	0.5622742154603921	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4769	0.4182632558930916	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2043	0.1791805057222869	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	0.162253517173877	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1791	0.1570789455450885	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1666	0.14611587006036708	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1524	0.13366181630972354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.770460387777135E-5	0.0
3	0.0	0.0	0.0	8.770460387777135E-5	0.0
4	0.0	0.0	0.0	8.770460387777135E-5	0.0
5	0.0	0.0	0.0	8.770460387777135E-5	0.0
6	0.0	0.0	0.0	8.770460387777135E-5	0.0
7	0.0	0.0	0.0	8.770460387777135E-5	0.0
8	0.0	0.0	0.0	8.770460387777135E-5	0.0
9	0.0	0.0	0.0	8.770460387777135E-5	0.0
10	0.0	0.0	0.0	1.754092077555427E-4	0.0
11	0.0	0.0	0.0	1.754092077555427E-4	0.0
12	0.0	0.0	0.0	1.754092077555427E-4	0.0
13	0.0	0.0	0.0	2.631138116333141E-4	0.0
14	8.770460387777135E-5	0.0	0.0	2.631138116333141E-4	0.0
15	8.770460387777135E-5	0.0	0.0	3.508184155110854E-4	0.0
16	8.770460387777135E-5	0.0	0.0	7.016368310221708E-4	0.0
17	8.770460387777135E-5	0.0	0.0	0.0011401598504110276	0.0
18	8.770460387777135E-5	0.0	0.0	0.0013155690581665702	0.0
19	8.770460387777135E-5	0.0	0.0	0.0013155690581665702	0.0
20	8.770460387777135E-5	0.0	0.0	0.0017540920775554272	0.0
21	8.770460387777135E-5	0.0	0.0	0.0028065473240886834	0.0
22	8.770460387777135E-5	0.0	0.0	0.00456063940164411	0.0
23	8.770460387777135E-5	0.0	0.0	0.00517457162878851	0.0
24	8.770460387777135E-5	0.0	0.0	0.007279482121855023	0.0
25	8.770460387777135E-5	0.0	0.0	0.010086029445943705	0.0
26	8.770460387777135E-5	0.0	0.0	0.016400760925143244	0.0
27	8.770460387777135E-5	0.0	0.0	0.053324399157684985	0.0
28	8.770460387777135E-5	0.0	0.0	0.14015195699667862	0.0
29	8.770460387777135E-5	0.0	0.0	0.26811297405434703	0.0
30	8.770460387777135E-5	0.0	0.0	0.4271214208847465	0.0
31	8.770460387777135E-5	0.0	0.0	0.931335188578054	8.770460387777135E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2755	0.0	24.039928	1
CGGTTTG	60	3.7262536E-5	21.583332	14
CAATCTA	55	5.1430584E-4	20.181818	9
CGTACAC	50	0.0070348475	18.5	3
ATTGGAC	65	0.0015799231	17.076923	3
CTTATAC	1250	0.0	16.576	37
TTCGTTA	80	3.3825354E-4	16.1875	22
AGTATCG	80	3.3825354E-4	16.1875	5
GTATCAA	4180	0.0	15.888756	2
GGCGTGT	100	1.09335124E-4	14.8	8
GGCGGGT	130	4.4435947E-6	14.230769	33
TCTTATA	1985	0.0	14.073048	37
TATCGGA	80	0.00629858	13.875	2
GTCGGTT	80	0.00629858	13.875	12
ATAGTAC	120	3.3003136E-5	13.874999	3
CGTTATG	95	0.0012450629	13.631579	24
GCTAGAC	95	0.0012450629	13.631579	3
TATCGTA	110	2.456332E-4	13.454545	7
CTTGTTA	770	0.0	13.214285	22
CGGGTTT	140	9.557889E-6	13.214285	35
>>END_MODULE