##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5736845
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28192673847733	33.0	31.0	34.0	30.0	34.0
2	32.42535923491048	34.0	31.0	34.0	31.0	34.0
3	32.4972313179108	34.0	31.0	34.0	30.0	34.0
4	36.01217986541383	37.0	35.0	37.0	35.0	37.0
5	35.93248763039615	37.0	35.0	37.0	35.0	37.0
6	35.99981418358	37.0	35.0	37.0	35.0	37.0
7	35.934639510044285	37.0	35.0	37.0	35.0	37.0
8	35.91240063135748	37.0	35.0	37.0	35.0	37.0
9	37.67099616601111	39.0	37.0	39.0	35.0	39.0
10	37.58820414356671	39.0	37.0	39.0	35.0	39.0
11	37.681007243528455	39.0	37.0	39.0	35.0	39.0
12	37.58576848424526	39.0	37.0	39.0	35.0	39.0
13	37.626442582987686	39.0	37.0	39.0	35.0	39.0
14	38.86297032602415	40.0	38.0	41.0	36.0	41.0
15	38.84122335534602	40.0	38.0	41.0	36.0	41.0
16	38.8242408501537	40.0	38.0	41.0	35.0	41.0
17	38.81937336637124	40.0	38.0	41.0	35.0	41.0
18	38.833825037978194	40.0	38.0	41.0	35.0	41.0
19	38.87014744166872	40.0	38.0	41.0	35.0	41.0
20	38.79455118623564	40.0	38.0	41.0	35.0	41.0
21	38.75950352502116	40.0	38.0	41.0	35.0	41.0
22	38.70165308632184	40.0	38.0	41.0	35.0	41.0
23	38.62327899742803	40.0	38.0	41.0	34.0	41.0
24	38.56025637785228	40.0	38.0	41.0	34.0	41.0
25	38.474099788298275	40.0	38.0	41.0	34.0	41.0
26	38.31475506136213	40.0	38.0	41.0	34.0	41.0
27	38.204646282059215	40.0	38.0	41.0	34.0	41.0
28	38.11691408779564	40.0	38.0	41.0	34.0	41.0
29	37.94024380996872	40.0	37.0	41.0	33.0	41.0
30	37.87290976137581	40.0	37.0	41.0	33.0	41.0
31	37.71052625615648	40.0	37.0	41.0	33.0	41.0
32	37.57825494675209	40.0	37.0	41.0	33.0	41.0
33	37.44246079508859	40.0	37.0	41.0	32.0	41.0
34	37.34582579797781	40.0	36.0	41.0	32.0	41.0
35	37.23756664159481	40.0	36.0	41.0	32.0	41.0
36	37.14637331146301	40.0	36.0	41.0	31.0	41.0
37	37.007656647512704	40.0	36.0	41.0	31.0	41.0
38	36.87733972244326	40.0	36.0	41.0	31.0	41.0
39	36.74653559578479	39.0	36.0	41.0	30.0	41.0
40	36.602645007839676	39.0	35.0	41.0	30.0	41.0
41	36.450789589051126	39.0	35.0	41.0	30.0	41.0
42	36.2298395720993	39.0	35.0	41.0	29.0	41.0
43	34.74165259824869	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	13.0
11	29.0
12	26.0
13	17.0
14	29.0
15	34.0
16	67.0
17	111.0
18	251.0
19	507.0
20	1070.0
21	2169.0
22	3946.0
23	6960.0
24	10856.0
25	17241.0
26	24919.0
27	36113.0
28	49889.0
29	66492.0
30	85715.0
31	107790.0
32	135403.0
33	175292.0
34	232762.0
35	313845.0
36	455791.0
37	773570.0
38	1430141.0
39	1805791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38475242053777	17.93492764751357	13.731798575697967	24.94852135625069
2	17.76218461541143	21.54607628408995	35.525327945935445	25.16641115456318
3	19.8654835541138	22.639952796354095	30.661783611026618	26.832780038505483
4	14.831967745337376	15.587888464826921	32.97176409681628	36.60837969301942
5	15.755733334262997	36.04026254849138	33.17142087680598	15.032583240439648
6	35.519105013295636	33.98157000929954	14.684186168529914	15.815138808874913
7	28.84531480282281	30.242197584212228	21.214430579874477	19.69805703309049
8	27.604702584783098	34.50616148771668	19.633613946341587	18.255521981158633
9	26.16364918347977	14.419598228643096	19.69171208216363	39.72504050571351
10	16.45522931158154	27.478239345842532	32.944222826309584	23.122308516266347
11	35.3100528252027	21.519946939476313	21.535774454425734	21.634225780895246
12	21.45151211162233	26.088067570241137	29.893242714418815	22.567177603717724
13	32.14991863994931	19.51722593167499	24.377580359936516	23.955275068439185
14	22.161815422937174	20.76873263963032	27.520876021576317	29.54857591585619
15	25.804619089412384	27.927963889559503	22.922686598644376	23.344730422383734
16	23.874620980695834	25.535533904088396	25.74486499112317	24.844980124092597
17	23.5778202130265	26.588272822431147	26.879809372573256	22.954097591969106
18	23.732277933254252	25.064769921446374	27.476252190881922	23.726699954417455
19	24.777364561880265	25.6741815405506	27.235876165383587	22.31257773218555
20	24.036957596030568	25.62370083207756	26.974443269776334	23.364898302115535
21	23.531592713416522	26.426023363015734	26.99727812063948	23.045105802928266
22	23.92178976423452	26.03676759612644	26.37610045242638	23.66534218721266
23	23.33259831841369	25.71017693523182	26.751393841039807	24.20583090531468
24	23.407831307975027	25.958850204249895	26.749650722653307	23.883667765121768
25	23.7394770121905	25.615159551983712	26.585082915783847	24.06028052004194
26	23.25560477928199	25.847726407110528	27.394813699864645	23.501855113742835
27	24.05498144014698	26.076423539419313	27.024854253513908	22.843740766919794
28	23.69680547408898	26.288543615872488	26.85720112710035	23.157449782938183
29	23.273297430904965	26.434878404419155	27.368527474596227	22.923296690079653
30	23.172301151591164	26.541121470076323	27.20847434434781	23.078103033984707
31	23.06339111480265	25.622323768552224	27.181159679231353	24.133125437413767
32	22.60646749214943	25.948809842343657	27.20303581498193	24.241686850524985
33	23.371731326190613	25.65235769835162	26.931039621952486	24.044871353505282
34	23.329879053730753	25.472938522829185	27.470761367964446	23.726421055475612
35	22.89653982284688	25.392214710350373	27.791320839241777	23.919924627560967
36	22.681212408562544	24.846670948927503	28.276134356078998	24.195982286430954
37	22.054805385189944	24.277717107573938	28.421597585432412	25.24587992180371
38	21.884415562909577	24.151114419162447	29.311930163704965	24.65253985422301
39	21.630478076364273	23.73431738176646	29.97874267127663	24.656461870592633
40	21.74200279073254	24.07990803307393	30.24727005871694	23.93081911747659
41	21.31493878604006	23.773310940072463	30.535756151682676	24.3759941222048
42	20.70097762794707	24.5028408471904	30.38286723800277	24.413314286859762
43	20.354358536791565	24.09533463079445	30.268030598700157	25.28227623371383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	753.0
1	1025.5
2	1298.0
3	5094.5
4	8891.0
5	8891.0
6	11937.0
7	14983.0
8	14695.0
9	14407.0
10	20072.0
11	25737.0
12	25737.0
13	38533.0
14	51329.0
15	78762.5
16	106196.0
17	86515.5
18	66835.0
19	66835.0
20	69346.5
21	71858.0
22	62242.0
23	52626.0
24	60726.5
25	68827.0
26	68827.0
27	77391.0
28	85955.0
29	94852.5
30	103750.0
31	113435.5
32	123121.0
33	123121.0
34	136633.0
35	150145.0
36	168371.0
37	186597.0
38	208606.5
39	230616.0
40	230616.0
41	255038.5
42	279461.0
43	308183.0
44	336905.0
45	370423.0
46	403941.0
47	403941.0
48	442393.5
49	480846.0
50	500839.5
51	520833.0
52	528385.5
53	535938.0
54	535938.0
55	496594.0
56	457250.0
57	414179.0
58	371108.0
59	339012.0
60	306916.0
61	306916.0
62	268499.5
63	230083.0
64	194001.0
65	157919.0
66	132138.5
67	106358.0
68	106358.0
69	87913.5
70	69469.0
71	56578.5
72	43688.0
73	35981.0
74	28274.0
75	28274.0
76	23009.5
77	17745.0
78	14617.5
79	11490.0
80	9083.0
81	6676.0
82	6676.0
83	5296.0
84	3916.0
85	3028.0
86	2140.0
87	1638.5
88	1137.0
89	1137.0
90	834.5
91	532.0
92	358.0
93	184.0
94	135.0
95	86.0
96	86.0
97	50.0
98	14.0
99	13.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5736845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.78975323770191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30290406936389	45.093558047067
2	9.268406376864801	10.156273965903306
3	3.019252858246315	4.962723571966253
4	1.4817195315066019	3.2473218999491995
5	0.8394866298820537	2.2997632648793855
6	0.5560035364698994	1.8277977937485228
7	0.39091969653725833	1.4992875596323587
8	0.2930383403130969	1.2844398683952234
9	0.22530043371077785	1.1109739650624662
>10	1.4636078992776946	15.001630851563913
>50	0.10986973640359919	4.11128877535622
>100	0.04547877441953128	4.443531177826042
>500	0.0019423801646279448	0.7503998927494634
>1k	0.0018149999979853114	1.7794960634523085
>5k	1.2736842089448335E-4	0.421727255546914
>10k+	1.2736842089448335E-4	2.009786046901458
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	56228	0.9801206063611619	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22634	0.3945374156003866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19958	0.3478915675776494	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16379	0.28550536052481806	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	8261	0.14399900990875647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.22935515949969E-5	1.7431183864998967E-5
2	0.0	0.0	0.0	6.972473545999587E-5	1.7431183864998967E-5
3	0.0	0.0	0.0	8.715591932499483E-5	1.7431183864998967E-5
4	0.0	0.0	0.0	1.045871031899938E-4	3.4862367729997934E-5
5	0.0	0.0	0.0	1.045871031899938E-4	3.4862367729997934E-5
6	0.0	0.0	0.0	1.045871031899938E-4	3.4862367729997934E-5
7	0.0	0.0	0.0	1.045871031899938E-4	3.4862367729997934E-5
8	0.0	0.0	0.0	1.2201828705499277E-4	3.4862367729997934E-5
9	0.0	0.0	0.0	1.2201828705499277E-4	3.4862367729997934E-5
10	0.0	0.0	0.0	1.5688065478499072E-4	3.4862367729997934E-5
11	0.0	0.0	0.0	2.963301257049824E-4	3.4862367729997934E-5
12	1.7431183864998967E-5	0.0	0.0	4.880731482199711E-4	1.045871031899938E-4
13	1.7431183864998967E-5	0.0	0.0	5.403666998149679E-4	1.5688065478499072E-4
14	1.7431183864998967E-5	0.0	0.0	9.064215609799463E-4	1.9174302251498865E-4
15	1.7431183864998967E-5	0.0	0.0	0.001568806547849907	2.4403657410998554E-4
16	1.7431183864998967E-5	0.0	0.0	0.0028587141538598307	2.7889894183998347E-4
17	1.7431183864998967E-5	0.0	0.0	0.004131190576004755	2.963301257049824E-4
18	3.4862367729997934E-5	0.0	0.0	0.004828437930604714	2.963301257049824E-4
19	5.22935515949969E-5	0.0	0.0	0.006658712236429606	2.963301257049824E-4
20	5.22935515949969E-5	0.0	0.0	0.008750454300229481	3.834860450299773E-4
21	5.22935515949969E-5	0.0	0.0	0.012411002911879264	4.183484127599752E-4
22	5.22935515949969E-5	0.0	0.0	0.01974953131904383	4.3577959662497416E-4
23	6.972473545999587E-5	0.0	0.0	0.024543106881918547	4.706419643549721E-4
24	6.972473545999587E-5	0.0	0.0	0.03341557946920302	4.706419643549721E-4
25	6.972473545999587E-5	0.0	0.0	0.04078897024409758	4.880731482199711E-4
26	6.972473545999587E-5	0.0	0.0	0.052938505398001864	5.22935515949969E-4
27	8.715591932499483E-5	0.0	0.0	0.0794513360566653	5.403666998149679E-4
28	8.715591932499483E-5	0.0	0.0	0.18944210624480878	5.403666998149679E-4
29	1.045871031899938E-4	0.0	0.0	0.4284759305855396	5.577978836799669E-4
30	1.045871031899938E-4	0.0	0.0	0.6867886442809593	5.577978836799669E-4
31	1.3944947091999174E-4	0.0	0.0	1.4792625563354074	5.577978836799669E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9350	0.0	18.163635	37
TATACAC	3260	0.0	16.96779	37
TCTTATA	14710	0.0	13.268186	37
TAGACAG	665	0.0	12.796992	5
TCGTTAC	500	0.0	12.21	23
TAATACC	670	0.0	12.149253	4
TATACCG	400	0.0	12.025001	5
TTAATAC	1095	0.0	11.995435	3
ATACCCG	430	0.0	11.616279	5
CTCTTAT	21060	0.0	11.542735	37
GTAATAC	435	0.0	11.482758	3
GGACCGT	355	0.0	11.464789	6
AACGCAG	36485	0.0	11.393586	5
TGTGCGA	390	0.0	11.384616	10
ACCCGTT	525	0.0	11.276191	7
CCTAGAC	545	0.0	11.201835	3
GAGTACT	20530	0.0	11.0567465	11
CTATACT	755	0.0	11.02649	4
TTATACA	5035	0.0	10.949355	36
GGAGTGT	1420	0.0	10.943663	6
>>END_MODULE