FastQCFastQC Report
Thu 9 Feb 2017
SRR2727616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168688
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT652341.262099782381912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT269570.5215443454896097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231610.44810211024538527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198530.3841013425457292No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT135360.261884640744421No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT93150.18021981593781633No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG86950.16822450881152046No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG78130.15116021706088664No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG71400.13813950464798805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.12635701748683612No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64670.12511879223508943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.10985379655340001No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG54510.10546196636361103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC2850.020.77192922
AACGACG4050.020.55555515
TAGATCG3250.018.7846155
CTTATAC92700.018.16073437
ACGCGCA3700.017.019
GCAATCG3500.016.91428623
TATACAC32650.016.3185337
AATCGGG3700.016.025
ACGACGC3950.015.9240516
ATACCCG5500.015.4727275
GGACCGT4100.015.3414636
GGAGTGT26000.015.2269236
AGTGTAC24400.015.08811427
GAGCGTT6200.014.6209687
TGGAGTG28800.014.4531255
CTCGTTA13500.014.11481522
TACTTAC22850.014.08752831
CGACGCG5000.013.6917
GGAGCGT7350.013.5918366
AGCGTTC6950.013.575548
Produced by FastQC (version 0.11.5)