FastQCFastQC Report
Thu 9 Feb 2017
SRR2727615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963993
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97180.3278685206071674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40740.1374497173238938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35680.12037815204017015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1101.7589628E-920.1818183
CTTATAC38200.018.93586337
CTACGGT609.2415075E-418.54
TCTAGCG852.725466E-517.41176428
CTAGCGG852.725466E-517.41176429
TCTTATA67050.016.05816537
TATACAC13050.015.45210637
TCGACTC750.004106439714.85
GGTATCA52400.014.7929391
TAGATCG1152.212061E-514.4782615
TCGTTAC3350.014.35820923
GCGATTC950.001245885713.63157913
AACGCAG88400.013.3936655
TCTATAC3600.013.3611123
CGCGTGA2501.0913936E-1113.32000110
ATCGATA1409.570189E-613.21428523
AGATCGA1551.8901846E-613.1290326
TTTACGC850.00940931313.0588233
CCGTATA850.00940931313.0588232
CGTTACG2602.3646862E-1112.80769324
Produced by FastQC (version 0.11.5)