FastQCFastQC Report
Thu 9 Feb 2017
SRR2727612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11329626
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1083700.9565187765244855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT460290.40627113375145835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT411510.3632158731453271No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307690.2715800150861114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC62600.018.35223437
CTTATAC183500.018.14713937
AACGCAG513100.015.2694415
ATACCCG9800.014.7244895
CGCAGAG566050.013.7986057
GGAGTGT43250.013.773416
CGTATAC3900.013.7564113
ACGCAGA575250.013.6293786
TCTTATA287700.013.36861237
GGTATCA366000.013.35942651
AGAGTAC587200.013.25434310
GAGTGTC40700.013.1363647
CAGAGTA596750.013.085639
GCAGAGT598200.013.0477278
TACTTAC32950.013.02579731
ACCCGTT11000.012.957
AGTGTAC41200.012.887135527
TCGTTAC16650.012.77777823
ACGGATG33750.012.66222328
CAACGCA636500.012.5038494
Produced by FastQC (version 0.11.5)