FastQCFastQC Report
Thu 9 Feb 2017
SRR2727611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7187684
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT904151.2579156234469964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT361440.5028601702579022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306450.4263543027211547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272820.3795659352859697No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT95440.1327826877197161No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86260.12001084076595465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78270.10889460360249559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC100750.017.93994937
TATACAC35800.016.9497237
TATACCG4450.014.1348325
TCTTATA155000.012.79483937
TCGTTAC7650.012.57516323
CGTTACG6300.012.33333324
TTATACA55550.012.22232237
CGTATAC3051.8189894E-1212.1311473
AACGCAG525500.012.0681265
GTACGAC2154.022695E-812.0465123
CTCTTAT228750.011.80765137
GTTCTAG7150.011.6433561
ACGGATG19150.011.30287328
GTCTTAG12350.011.2348181
GTTACGG14700.011.20068125
TACTTAC14850.011.08754231
ACCCGAC21200.011.08254711
GAGTACT328100.011.07406311
CGCAGAG571050.011.0115587
TACGGAT20100.010.95273627
Produced by FastQC (version 0.11.5)