FastQCFastQC Report
Thu 9 Feb 2017
SRR2727610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9437959
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT719960.7628344221457203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT244180.25872119173223784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237020.2511348057350111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193430.20494897254798414No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT127010.13457358736142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC141850.018.68910837
TATACAC49600.017.90322737
TACTTAC26800.016.22201531
GTACTTA29950.015.38063330
AGTGTAC35250.015.16737627
TCTTATA230300.014.31480737
ATACCCG9250.014.25
TACGGAT35000.013.95428627
CATTACG13400.013.52985124
ACGGATG36250.013.47310428
TCGTTAC19950.013.446115523
TTACGGA37500.013.12266726
CGTTACG17750.013.02816924
GTTACGG27350.012.85191925
ATTACGG14400.012.84722225
GTGTACT36950.012.81732128
GAGTGTC37650.012.6772917
ACCCGTT10700.012.6214957
GGAGTGT41850.012.554366
CTCGTTA19900.012.55025122
Produced by FastQC (version 0.11.5)