##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727610.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9437959 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27006220306742 33.0 31.0 34.0 30.0 34.0 2 32.42306583446697 34.0 31.0 34.0 31.0 34.0 3 32.495286533878776 34.0 31.0 34.0 30.0 34.0 4 36.01489813634495 37.0 35.0 37.0 35.0 37.0 5 35.92485938962015 37.0 35.0 37.0 35.0 37.0 6 35.98449135030148 37.0 35.0 37.0 35.0 37.0 7 35.9154279013079 37.0 35.0 37.0 35.0 37.0 8 35.89222479139823 37.0 35.0 37.0 35.0 37.0 9 37.656191132002164 39.0 37.0 39.0 35.0 39.0 10 37.56449567115094 39.0 37.0 39.0 35.0 39.0 11 37.66159410101273 39.0 37.0 39.0 35.0 39.0 12 37.56427708575551 39.0 37.0 39.0 35.0 39.0 13 37.60209638545791 39.0 37.0 39.0 35.0 39.0 14 38.83995098940353 40.0 38.0 41.0 36.0 41.0 15 38.81719776489811 40.0 38.0 41.0 35.0 41.0 16 38.79534229805406 40.0 38.0 41.0 35.0 41.0 17 38.77828850496172 40.0 38.0 41.0 35.0 41.0 18 38.78561233419217 40.0 38.0 41.0 35.0 41.0 19 38.82626127110745 40.0 38.0 41.0 35.0 41.0 20 38.74757307167789 40.0 38.0 41.0 35.0 41.0 21 38.713018354921864 40.0 38.0 41.0 35.0 41.0 22 38.65959504592042 40.0 38.0 41.0 34.0 41.0 23 38.58258390399874 40.0 38.0 41.0 34.0 41.0 24 38.527019136234856 40.0 38.0 41.0 34.0 41.0 25 38.43989383721629 40.0 38.0 41.0 34.0 41.0 26 38.286994889467096 40.0 38.0 41.0 34.0 41.0 27 38.182562246773905 40.0 38.0 41.0 34.0 41.0 28 38.09297306758803 40.0 38.0 41.0 33.0 41.0 29 37.92788207704653 40.0 37.0 41.0 33.0 41.0 30 37.878380378639065 40.0 37.0 41.0 33.0 41.0 31 37.73202627813916 40.0 37.0 41.0 33.0 41.0 32 37.607702894238045 40.0 37.0 41.0 33.0 41.0 33 37.48951240411195 40.0 37.0 41.0 32.0 41.0 34 37.405400680380154 40.0 37.0 41.0 32.0 41.0 35 37.309622239299834 40.0 37.0 41.0 32.0 41.0 36 37.230557581358426 40.0 36.0 41.0 31.0 41.0 37 37.12205689810689 40.0 36.0 41.0 31.0 41.0 38 37.01543289179366 40.0 36.0 41.0 31.0 41.0 39 36.89995093218778 39.0 36.0 41.0 31.0 41.0 40 36.785914306260494 39.0 36.0 41.0 30.0 41.0 41 36.65079515602897 39.0 36.0 41.0 30.0 41.0 42 36.47037659307484 39.0 35.0 41.0 30.0 41.0 43 34.93543010729333 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 20.0 10 42.0 11 56.0 12 51.0 13 36.0 14 40.0 15 41.0 16 71.0 17 194.0 18 361.0 19 891.0 20 1643.0 21 3383.0 22 6551.0 23 11245.0 24 18003.0 25 27381.0 26 39969.0 27 57630.0 28 79413.0 29 106079.0 30 137004.0 31 174239.0 32 220757.0 33 285827.0 34 379871.0 35 514836.0 36 750833.0 37 1253411.0 38 2430523.0 39 2937554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.12476881919067 17.453392200580655 13.618272764270326 25.803566215958345 2 17.714062966368047 20.893850036856485 35.935131737698796 25.45695525907667 3 19.4346574296413 22.051600351304767 30.834675166527 27.67906705252693 4 14.921838503430667 15.177571761013159 32.919077101309725 36.98151263424645 5 15.710504781807169 35.78080811751778 33.41509536118985 15.093591739485202 6 36.044350266832055 33.85132315154156 14.515733751333313 15.588592830293074 7 29.265479962352032 30.329089160060985 20.85594989340386 19.549480984183127 8 27.52291040891362 34.953595369507326 19.444564232584607 18.078929988994442 9 26.736628120550215 14.108082054605239 18.855252496858697 40.30003732798585 10 16.243437802601175 27.228937951521086 32.978570896525405 23.549053349352334 11 36.29655521919516 20.9983535635194 21.05699971784154 21.648091499443893 12 21.52295851253433 25.204114576043402 29.938856483695258 23.33407042772701 13 32.57622755089316 18.985089890727433 23.851735316926042 24.58694724145337 14 22.3378062990102 20.053837911353504 27.010288983031185 30.59806680660511 15 26.447073991315285 27.355045725458226 22.24798815082795 23.94989213239854 16 24.43652276938266 25.257876199716485 25.15416733639127 25.151433694509585 17 24.12986748512046 26.049498625709223 26.104468137655612 23.71616575151471 18 24.225364827289457 24.932297332505897 26.640018249708437 24.202319590496206 19 25.46573893783603 25.317677264756078 26.375617863989447 22.840965933418445 20 24.68012416667629 24.932170186371863 26.10045243892244 24.287253208029405 21 23.990600086311034 26.034717887628034 26.295992597552075 23.678689428508854 22 24.856030843109195 25.237183166402822 25.32729798889781 24.579488001590175 23 23.953685325397153 25.004897775038014 25.946616212255215 25.094800687309622 24 23.94209383617793 25.109602616413145 26.229007775939696 24.719295771469234 25 24.577686764691393 24.62278126022798 25.695619148165406 25.103912826915227 26 23.795473152617 25.013554307663345 26.8717314834701 24.319241056249556 27 24.874784897878875 25.393488147172498 26.282949523302655 23.448777431645972 28 24.418743501640556 25.743712173362905 25.766524308910434 24.071020016086106 29 23.98759096113895 25.69259942748215 26.657458461093125 23.662351150285776 30 24.322334945510995 25.464626409163255 26.329198929556696 23.883839715769057 31 24.017311369968866 24.75332855334506 26.55973606157857 24.6696240151075 32 23.356299810160227 25.182775216548407 26.384899531773765 25.0760254415176 33 24.05418374883807 24.593940278825112 26.169090160277236 25.182785812059578 34 24.223425848745475 24.658837784737145 26.996578391578097 24.121157974939287 35 23.70139560894469 24.479084937749782 27.088271945237313 24.731247508068215 36 23.498322041873674 23.997667292260967 27.622656551061514 24.881354114803848 37 22.518576315069815 23.326526423774464 28.239601379916994 25.91529588123873 38 22.780825812021437 23.090585581056246 28.684199624092454 25.444388982829867 39 22.151420662030848 22.784523645419526 29.740805188918497 25.323250503631133 40 22.54908079172626 22.865367395641368 29.947385870186555 24.63816594244582 41 21.96101932631833 22.856202278479913 30.29172938767799 24.891049007523765 42 21.249880403167676 23.3988090009715 30.340044918610047 25.011265677250773 43 20.815146579890843 23.010398752526896 30.00634988984377 26.168104777738492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 464.0 1 770.0 2 1076.0 3 5482.5 4 9889.0 5 9889.0 6 13702.0 7 17515.0 8 16774.5 9 16034.0 10 21594.0 11 27154.0 12 27154.0 13 41317.5 14 55481.0 15 89911.5 16 124342.0 17 97486.5 18 70631.0 19 70631.0 20 72125.5 21 73620.0 22 63640.0 23 53660.0 24 64035.0 25 74410.0 26 74410.0 27 88971.0 28 103532.0 29 119056.0 30 134580.0 31 156980.5 32 179381.0 33 179381.0 34 207285.0 35 235189.0 36 273253.5 37 311318.0 38 352958.5 39 394599.0 40 394599.0 41 441284.0 42 487969.0 43 544180.5 44 600392.0 45 660241.0 46 720090.0 47 720090.0 48 783775.0 49 847460.0 50 876993.0 51 906526.0 52 920086.5 53 933647.0 54 933647.0 55 858660.0 56 783673.0 57 705244.5 58 626816.0 59 570093.0 60 513370.0 61 513370.0 62 448481.5 63 383593.0 64 323423.5 65 263254.0 66 221159.0 67 179064.0 68 179064.0 69 146866.5 70 114669.0 71 94180.5 72 73692.0 73 60837.5 74 47983.0 75 47983.0 76 39232.5 77 30482.0 78 24759.5 79 19037.0 80 14975.5 81 10914.0 82 10914.0 83 8518.0 84 6122.0 85 4722.5 86 3323.0 87 2524.0 88 1725.0 89 1725.0 90 1266.0 91 807.0 92 544.5 93 282.0 94 209.0 95 136.0 96 136.0 97 86.5 98 37.0 99 29.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 9437959.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.068714617868785 #Duplication Level Percentage of deduplicated Percentage of total 1 78.54089323220879 38.53900675843755 2 10.896716992890264 10.693757927916273 3 3.760929147735844 5.536318771448237 4 1.8275033301041959 3.5869295747235053 5 1.1021752253205583 2.7041160795069854 6 0.7279448577329147 2.143159108898489 7 0.5105125388634754 1.753513585483486 8 0.35873237090845916 1.408202906583891 9 0.28015424795904603 1.2372127957877472 >10 1.7737815601767866 16.2387347165458 >50 0.1465083466520611 4.961876702433079 >100 0.0685604824447517 6.016494003276202 >500 0.0032712084436511144 1.0760312043980864 >1k 0.002035019778869879 1.9076704146199435 >5k 1.7319309569499423E-4 0.5805868859993135 >10k+ 1.082456848093714E-4 1.6163885639414417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 71996 0.7628344221457203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 24418 0.25872119173223784 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 23702 0.2511348057350111 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19343 0.20494897254798414 No Hit GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT 12701 0.13457358736142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.238204467724431E-5 2 0.0 0.0 0.0 0.0 4.238204467724431E-5 3 0.0 0.0 0.0 0.0 4.238204467724431E-5 4 0.0 0.0 0.0 0.0 4.238204467724431E-5 5 0.0 1.0595511169311077E-5 0.0 0.0 4.238204467724431E-5 6 1.0595511169311077E-5 1.0595511169311077E-5 0.0 0.0 6.357306701586646E-5 7 4.238204467724431E-5 1.0595511169311077E-5 0.0 2.1191022338622154E-5 7.416857818517754E-5 8 7.416857818517754E-5 1.0595511169311077E-5 0.0 2.1191022338622154E-5 7.416857818517754E-5 9 7.416857818517754E-5 1.0595511169311077E-5 0.0 4.238204467724431E-5 1.1655062286242184E-4 10 1.0595511169311077E-4 1.0595511169311077E-5 0.0 9.53596005237997E-5 1.1655062286242184E-4 11 1.1655062286242184E-4 1.0595511169311077E-5 0.0 2.0131471221691047E-4 1.1655062286242184E-4 12 1.1655062286242184E-4 1.0595511169311077E-5 0.0 3.814384020951988E-4 1.1655062286242184E-4 13 1.1655062286242184E-4 1.0595511169311077E-5 0.0 4.7679800261899845E-4 1.1655062286242184E-4 14 1.2714613403173292E-4 1.0595511169311077E-5 0.0 8.26449871206264E-4 2.0131471221691047E-4 15 1.4833715637035507E-4 1.0595511169311077E-5 0.0 0.0012820568514866403 2.3310124572484367E-4 16 1.6952817870897723E-4 1.0595511169311077E-5 0.0 0.0024369675689415475 2.75483290402088E-4 17 1.6952817870897723E-4 1.0595511169311077E-5 0.0 0.0035494962417192107 2.860788015713991E-4 18 1.907192010475994E-4 1.0595511169311077E-5 0.0 0.004344159579417541 3.496518685872655E-4 19 2.0131471221691047E-4 1.0595511169311077E-5 0.0 0.006749340614851156 3.496518685872655E-4 20 2.5429226806346583E-4 1.0595511169311077E-5 0.0 0.009090948583268903 3.602473797565766E-4 21 2.6488777923277694E-4 1.0595511169311077E-5 0.0 0.013541063274379556 3.814384020951988E-4 22 2.75483290402088E-4 1.0595511169311077E-5 0.0 0.019728841797257226 3.814384020951988E-4 23 2.75483290402088E-4 1.0595511169311077E-5 0.0 0.024496821823447208 6.357306701586646E-4 24 2.75483290402088E-4 1.0595511169311077E-5 0.0 0.03363015245139336 6.463261813279757E-4 25 2.860788015713991E-4 1.0595511169311077E-5 0.0 0.04047485266676831 6.463261813279757E-4 26 2.860788015713991E-4 1.0595511169311077E-5 0.0 0.05263849948913743 6.463261813279757E-4 27 2.860788015713991E-4 1.0595511169311077E-5 0.0 0.07281235275550572 6.569216924972867E-4 28 2.9667431274071015E-4 1.0595511169311077E-5 0.0 0.16952817870897724 6.569216924972867E-4 29 2.9667431274071015E-4 1.0595511169311077E-5 0.0 0.4226443450326495 6.675172036665979E-4 30 2.9667431274071015E-4 1.0595511169311077E-5 0.0 0.6680363837138941 6.675172036665979E-4 31 2.9667431274071015E-4 1.0595511169311077E-5 0.0 1.4517333673519879 6.8870822600522E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 14185 0.0 18.689108 37 TATACAC 4960 0.0 17.903227 37 TACTTAC 2680 0.0 16.222015 31 GTACTTA 2995 0.0 15.380633 30 AGTGTAC 3525 0.0 15.167376 27 TCTTATA 23030 0.0 14.314807 37 ATACCCG 925 0.0 14.2 5 TACGGAT 3500 0.0 13.954286 27 CATTACG 1340 0.0 13.529851 24 ACGGATG 3625 0.0 13.473104 28 TCGTTAC 1995 0.0 13.4461155 23 TTACGGA 3750 0.0 13.122667 26 CGTTACG 1775 0.0 13.028169 24 GTTACGG 2735 0.0 12.851919 25 ATTACGG 1440 0.0 12.847222 25 GTGTACT 3695 0.0 12.817321 28 GAGTGTC 3765 0.0 12.677291 7 ACCCGTT 1070 0.0 12.621495 7 GGAGTGT 4185 0.0 12.55436 6 CTCGTTA 1990 0.0 12.550251 22 >>END_MODULE