FastQCFastQC Report
Thu 9 Feb 2017
SRR2727609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7270448
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT652010.8967948054920412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT222030.30538695827272266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220490.30326879444017757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173430.2385410087521429No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT92730.1275437222025383No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG77580.10670594164211064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC118150.019.0558637
TATACAC38150.016.97247737
TACTTAC18050.015.47645431
TATACCG3550.015.1126765
TCTTATA185550.013.70924337
AGTGTAC22900.013.65283827
GTACTTA21200.013.61320830
TACGGAT18200.013.01098827
CGTATAC2850.012.9824563
TCGTTAC10150.012.940886523
GTTACGG15550.012.61093325
ACGGATG18500.012.528
TGTTACG8300.012.48192724
TTACGGA19150.012.46214226
ATACCCG7100.012.2464795
ACCCGTT7150.012.1608397
TAGATCG2451.5661499E-912.0816335
CTACAGT28900.011.97058823
GAGTACT216350.011.87728211
GTCTTAC6100.011.8278691
Produced by FastQC (version 0.11.5)