FastQCFastQC Report
Thu 9 Feb 2017
SRR2727608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005008
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60330.3008965550262144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29830.1487774612370624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28160.14044831741319735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG801.6176493E-518.52
GTATAGA1900.017.5263161
CGCGTAT650.001580488817.0769237
TATACAC8500.016.54117837
ATACGCG750.004105822614.84
TATCGCA750.004105822614.88
TATACGC1252.961222E-614.7999993
GGACCGT2151.2732926E-1114.6279076
CTTATAC22350.014.48545937
ATAAGAC1803.3342076E-914.3888883
GTTCTAA1956.730261E-1014.230771
ATTAGAC1102.4577897E-413.4545453
TCTTATA34500.013.13768237
TAGACAG3150.012.9206345
TATACTG2305.7661964E-1012.8695655
GTGTTAG2451.1459633E-1012.8367361
ATAGGAC2451.1459633E-1012.8367363
TATGCCG1307.002884E-512.8076935
CCCTATA2753.6379788E-1212.7818182
TAGGGTG3350.012.7014925
Produced by FastQC (version 0.11.5)