##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2005008
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.149106138229875	34.0	33.0	34.0	31.0	34.0
2	33.32764707173238	34.0	33.0	34.0	31.0	34.0
3	33.415516546567396	34.0	34.0	34.0	31.0	34.0
4	36.6097222554723	37.0	37.0	37.0	35.0	37.0
5	36.59485248936662	37.0	37.0	37.0	35.0	37.0
6	36.623571576771766	37.0	37.0	37.0	35.0	37.0
7	36.630213445532384	37.0	37.0	37.0	35.0	37.0
8	36.6088983186102	37.0	37.0	37.0	35.0	37.0
9	38.27498394021371	39.0	39.0	39.0	37.0	39.0
10	38.37968726309322	39.0	39.0	39.0	37.0	39.0
11	38.479886364543184	39.0	39.0	39.0	37.0	39.0
12	38.46076075506931	39.0	39.0	39.0	37.0	39.0
13	38.49763093214591	39.0	39.0	39.0	37.0	39.0
14	40.0811966835045	41.0	40.0	41.0	38.0	41.0
15	40.102566174299554	41.0	40.0	41.0	38.0	41.0
16	40.10300856654936	41.0	40.0	41.0	38.0	41.0
17	40.1007906202868	41.0	40.0	41.0	38.0	41.0
18	40.08384904199883	41.0	40.0	41.0	38.0	41.0
19	40.119634435373825	41.0	40.0	41.0	38.0	41.0
20	40.09143205413644	41.0	40.0	41.0	38.0	41.0
21	40.05437783789392	41.0	40.0	41.0	38.0	41.0
22	40.02655500626432	41.0	40.0	41.0	38.0	41.0
23	39.98524095664456	41.0	40.0	41.0	38.0	41.0
24	39.99052422733475	41.0	40.0	41.0	38.0	41.0
25	39.95142164021291	41.0	40.0	41.0	38.0	41.0
26	39.88269572989235	41.0	40.0	41.0	38.0	41.0
27	39.85165695099471	41.0	40.0	41.0	38.0	41.0
28	39.820048099558704	41.0	40.0	41.0	38.0	41.0
29	39.76429620230942	41.0	40.0	41.0	38.0	41.0
30	39.69151793908054	41.0	40.0	41.0	37.0	41.0
31	39.630217435541404	41.0	40.0	41.0	37.0	41.0
32	39.55713692912946	41.0	40.0	41.0	37.0	41.0
33	39.451080993193045	41.0	40.0	41.0	36.0	41.0
34	39.39918593841022	41.0	40.0	41.0	36.0	41.0
35	39.34947591231556	41.0	39.0	41.0	36.0	41.0
36	39.313212715360734	41.0	39.0	41.0	35.0	41.0
37	39.2128250859847	41.0	39.0	41.0	35.0	41.0
38	39.17065817193747	41.0	39.0	41.0	35.0	41.0
39	39.09558116476343	41.0	39.0	41.0	35.0	41.0
40	39.02900586930326	41.0	39.0	41.0	35.0	41.0
41	38.92817135891727	41.0	39.0	41.0	35.0	41.0
42	38.807606253940136	41.0	39.0	41.0	35.0	41.0
43	37.62465536297112	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	3.0
14	2.0
15	0.0
16	5.0
17	10.0
18	23.0
19	40.0
20	76.0
21	170.0
22	308.0
23	542.0
24	965.0
25	1404.0
26	2087.0
27	2891.0
28	4235.0
29	5960.0
30	7852.0
31	9944.0
32	12431.0
33	16198.0
34	24270.0
35	38169.0
36	64088.0
37	136229.0
38	343704.0
39	1333401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73483397572478	16.922725495359618	11.765339589667473	26.577100939248123
2	18.499377558593284	19.479672899060752	34.80420028249264	27.21674925985333
3	19.536829778235298	20.69887002944627	29.65738790069666	30.10691229162178
4	14.491862376608971	14.41166319535882	33.40615099790126	37.69032343013095
5	16.185970330292946	34.75861442946861	33.426749419453685	15.628665820784754
6	37.11551275605883	33.22869534685148	14.449867531700622	15.205924365389064
7	31.50426332463511	29.40915946470039	19.831043068157335	19.255534142507162
8	28.592205118383568	33.30455539329519	18.876533160964946	19.2267063273563
9	27.222534772928586	13.51136753569063	18.33728344226058	40.92881424912021
10	16.56177930462123	25.68533392385467	32.977324778754	24.775561992770104
11	37.375761094220074	20.167650203889462	21.177870611987583	21.278718089902885
12	22.617216489909268	23.864842434543903	29.09404850254961	24.423892572997215
13	32.347501855354196	18.472245497274827	23.42220080917383	25.758051838197154
14	23.06609250436906	19.20067151851763	25.03291757439372	32.70031840271959
15	26.599444989745674	26.476203586220105	21.190489015505175	25.733862408529045
16	26.05361175616257	24.72169687103493	23.40973203099439	25.814959341808112
17	25.333115877841884	25.231819523912126	24.579802175352917	24.855262422893077
18	25.506731145212385	24.19431742915739	25.075411170429245	25.223540255200977
19	26.22468339278447	24.295264657298127	24.991471355725263	24.488580594192143
20	25.689872559111983	24.170028249263844	24.87895310143401	25.261146090190163
21	25.533763456305415	24.530326063536904	24.828329862025488	25.107580618132197
22	25.91919832738822	24.17950452068022	24.29421727993105	25.60707987200051
23	25.394611891822876	24.07880666810307	24.61396662756458	25.912614812509478
24	25.43660673673123	24.294366905269204	24.49526385929632	25.773762498703245
25	25.549474116811506	23.789531014340092	24.532271192932896	26.128723675915506
26	25.12149577458045	24.238556654138037	25.053366370608	25.586581200673514
27	25.71904950005187	24.30204767262774	24.701347825046085	25.277555002274305
28	25.462940796246198	24.34758365053905	24.733417522523602	25.456058030691146
29	25.28453751805479	24.633417921524504	24.810474571672533	25.271569988748176
30	25.0884784499613	24.639801935952374	24.80074892469257	25.470970689393756
31	25.34827791210808	23.93950547828238	24.832369746155628	25.87984686345391
32	24.650674710524846	24.41197242105767	24.947531381420923	25.98982148699656
33	25.085934819212692	23.957161268184464	25.08683257124161	25.870071341361232
34	25.375659348990126	23.801451166279637	25.375260348088386	25.447629136641847
35	24.924588829570755	24.084342805614742	25.47800308028696	25.513065284527542
36	24.821048095568695	23.503746618467357	25.82059522954522	25.854610056418725
37	24.62833066002729	23.183498519706657	25.900445285006345	26.28772553525971
38	24.056512492718234	23.20539369418975	26.766925618251896	25.97116819484012
39	23.957610144198927	22.899858753680782	27.06822117417985	26.074309927940437
40	23.914518046810787	22.975319799222746	27.50258353083878	25.607578623127687
41	23.59940708466001	22.76200394212891	27.891609410037265	25.74697956317381
42	23.210929831701417	23.032775929073598	27.828417642223872	25.92787659700111
43	22.87108081364264	22.686642646812384	28.02637196460064	26.41590457494434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	128.0
2	157.0
3	625.0
4	1093.0
5	1093.0
6	1374.5
7	1656.0
8	1583.5
9	1511.0
10	1960.5
11	2410.0
12	2410.0
13	3775.5
14	5141.0
15	8210.5
16	11280.0
17	9870.0
18	8460.0
19	8460.0
20	9408.5
21	10357.0
22	9740.5
23	9124.0
24	11179.0
25	13234.0
26	13234.0
27	16271.5
28	19309.0
29	22507.0
30	25705.0
31	30669.5
32	35634.0
33	35634.0
34	41810.0
35	47986.0
36	55372.0
37	62758.0
38	72160.0
39	81562.0
40	81562.0
41	90531.5
42	99501.0
43	109242.5
44	118984.0
45	129258.0
46	139532.0
47	139532.0
48	151507.5
49	163483.0
50	170640.5
51	177798.0
52	178851.0
53	179904.0
54	179904.0
55	174689.5
56	169475.0
57	159007.0
58	148539.0
59	137798.5
60	127058.0
61	127058.0
62	113668.5
63	100279.0
64	86608.0
65	72937.0
66	63337.5
67	53738.0
68	53738.0
69	45685.0
70	37632.0
71	32304.0
72	26976.0
73	22603.0
74	18230.0
75	18230.0
76	15543.0
77	12856.0
78	10728.0
79	8600.0
80	7255.0
81	5910.0
82	5910.0
83	4642.0
84	3374.0
85	2640.5
86	1907.0
87	1249.5
88	592.0
89	592.0
90	379.0
91	166.0
92	102.0
93	38.0
94	27.5
95	17.0
96	17.0
97	10.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2005008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.37867660312664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.7951026254983	54.589964894558896
2	8.010006326199406	10.3134721372678
3	2.6398323342437435	5.098467363982646
4	1.2962491393832416	3.3380321656573972
5	0.751576437591046	2.419274820910197
6	0.4848017692289224	1.8726537790687454
7	0.35097596112477214	1.5816757527706278
8	0.262974806666306	1.354397602651189
9	0.21058734455944242	1.2201601096893187
>10	1.1187768800902305	12.897466579226464
>50	0.05941577603518044	2.592670413049229
>100	0.01907816173878171	1.9147201101550018
>500	3.1121882029869647E-4	0.13293790313225504
>1k	2.3341411522401123E-4	0.371915573005636
>5k	7.78047050746704E-5	0.30219079487447836
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6033	0.3008965550262144	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2983	0.1487774612370624	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2816	0.14044831741319735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.987511271775474E-5
2	0.0	0.0	0.0	0.0	4.987511271775474E-5
3	0.0	0.0	0.0	0.0	4.987511271775474E-5
4	0.0	0.0	0.0	0.0	4.987511271775474E-5
5	0.0	0.0	0.0	0.0	4.987511271775474E-5
6	0.0	0.0	0.0	0.0	1.4962533815326422E-4
7	0.0	0.0	0.0	0.0	1.4962533815326422E-4
8	0.0	0.0	0.0	4.987511271775474E-5	1.4962533815326422E-4
9	0.0	0.0	0.0	1.4962533815326422E-4	2.493755635887737E-4
10	4.987511271775474E-5	0.0	0.0	1.4962533815326422E-4	2.493755635887737E-4
11	4.987511271775474E-5	0.0	0.0	3.491257890242832E-4	2.493755635887737E-4
12	4.987511271775474E-5	0.0	0.0	5.486262398953022E-4	4.488760144597927E-4
13	4.987511271775474E-5	0.0	0.0	5.985013526130569E-4	4.488760144597927E-4
14	4.987511271775474E-5	0.0	0.0	8.478769162018307E-4	4.987511271775474E-4
15	4.987511271775474E-5	0.0	0.0	0.0012468778179438686	4.987511271775474E-4
16	4.987511271775474E-5	0.0	0.0	0.0019950045087101897	5.985013526130569E-4
17	4.987511271775474E-5	0.0	0.0	0.003441382777525077	5.985013526130569E-4
18	4.987511271775474E-5	0.0	0.0	0.004139634355573644	5.985013526130569E-4
19	4.987511271775474E-5	0.0	0.0	0.005835388187977305	5.985013526130569E-4
20	4.987511271775474E-5	0.0	0.0	0.007730642471251985	6.982515780485664E-4
21	4.987511271775474E-5	0.0	0.0	0.01082289945975278	6.982515780485664E-4
22	4.987511271775474E-5	0.0	0.0	0.017605914789367422	6.982515780485664E-4
23	4.987511271775474E-5	0.0	0.0	0.0231420523010382	7.481266907663211E-4
24	4.987511271775474E-5	0.0	0.0	0.031920072139363036	7.481266907663211E-4
25	4.987511271775474E-5	0.0	0.0	0.038254211454517885	7.980018034840758E-4
26	4.987511271775474E-5	0.0	0.0	0.046832730841971704	8.478769162018307E-4
27	4.987511271775474E-5	0.0	0.0	0.06603464923830728	8.977520289195854E-4
28	4.987511271775474E-5	0.0	0.0	0.1571564801736452	8.977520289195854E-4
29	4.987511271775474E-5	0.0	0.0	0.30568456584711884	8.977520289195854E-4
30	4.987511271775474E-5	0.0	0.0	0.49211773718608604	8.977520289195854E-4
31	4.987511271775474E-5	0.0	0.0	0.9685248138660794	8.977520289195854E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	80	1.6176493E-5	18.5	2
GTATAGA	190	0.0	17.526316	1
CGCGTAT	65	0.0015804888	17.076923	7
TATACAC	850	0.0	16.541178	37
ATACGCG	75	0.0041058226	14.8	4
TATCGCA	75	0.0041058226	14.8	8
TATACGC	125	2.961222E-6	14.799999	3
GGACCGT	215	1.2732926E-11	14.627907	6
CTTATAC	2235	0.0	14.485459	37
ATAAGAC	180	3.3342076E-9	14.388888	3
GTTCTAA	195	6.730261E-10	14.23077	1
ATTAGAC	110	2.4577897E-4	13.454545	3
TCTTATA	3450	0.0	13.137682	37
TAGACAG	315	0.0	12.920634	5
TATACTG	230	5.7661964E-10	12.869565	5
GTGTTAG	245	1.1459633E-10	12.836736	1
ATAGGAC	245	1.1459633E-10	12.836736	3
TATGCCG	130	7.002884E-5	12.807693	5
CCCTATA	275	3.6379788E-12	12.781818	2
TAGGGTG	335	0.0	12.701492	5
>>END_MODULE