FastQCFastQC Report
Thu 9 Feb 2017
SRR2727607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039964
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT1254.129106E-1019.2400024
ATAAGAC1301.3948011E-817.0769253
CTTATAC27100.017.06642237
TATAGAT1201.0423355E-716.9583342
GTATAGA1452.9831426E-916.5862061
TATACAC9800.016.4234737
ATACACA7250.015.31034537
GTAGTAC2109.094947E-1214.976193
CGTCTAT750.00410585614.81
ATTGGAC1508.110328E-814.83
ACGATCG800.00630082813.8755
TAACCGA800.00630082813.8755
GTATAAA1601.7852835E-713.8751
TCTATAT800.00630082813.8753
TATACTG1753.5697667E-813.7428585
TACTTAC1652.597717E-713.45454531
TATATGG1102.4578228E-413.4545452
GTATATG1652.597717E-713.4545451
CTATTAC1102.4578228E-413.4545451
CCCTATA1951.0270014E-813.2820522
Produced by FastQC (version 0.11.5)