##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2039964
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13164251918171	34.0	33.0	34.0	31.0	34.0
2	33.31504624591415	34.0	33.0	34.0	31.0	34.0
3	33.4006286385446	34.0	34.0	34.0	31.0	34.0
4	36.60085668178458	37.0	37.0	37.0	35.0	37.0
5	36.57795088540778	37.0	37.0	37.0	35.0	37.0
6	36.61109950959919	37.0	37.0	37.0	35.0	37.0
7	36.61708343872735	37.0	37.0	37.0	35.0	37.0
8	36.59258104554786	37.0	37.0	37.0	35.0	37.0
9	38.260456557076495	39.0	39.0	39.0	37.0	39.0
10	38.362494142053485	39.0	39.0	39.0	37.0	39.0
11	38.465898908019945	39.0	39.0	39.0	37.0	39.0
12	38.44057787294285	39.0	39.0	39.0	37.0	39.0
13	38.482132527828924	39.0	39.0	39.0	37.0	39.0
14	40.063806518154244	41.0	40.0	41.0	38.0	41.0
15	40.08457551211688	41.0	40.0	41.0	38.0	41.0
16	40.07928571288513	41.0	40.0	41.0	38.0	41.0
17	40.07169685347389	41.0	40.0	41.0	38.0	41.0
18	40.04969548482228	41.0	40.0	41.0	38.0	41.0
19	40.08117986395838	41.0	40.0	41.0	38.0	41.0
20	40.05417007358953	41.0	40.0	41.0	38.0	41.0
21	40.01524683768929	41.0	40.0	41.0	38.0	41.0
22	39.99115229484442	41.0	40.0	41.0	38.0	41.0
23	39.946464741534655	41.0	40.0	41.0	38.0	41.0
24	39.952067781588305	41.0	40.0	41.0	38.0	41.0
25	39.90935232190372	41.0	40.0	41.0	38.0	41.0
26	39.84661445005892	41.0	40.0	41.0	38.0	41.0
27	39.813623181585555	41.0	40.0	41.0	38.0	41.0
28	39.777815196738764	41.0	40.0	41.0	38.0	41.0
29	39.727563819753684	41.0	40.0	41.0	37.0	41.0
30	39.661053332313706	41.0	40.0	41.0	37.0	41.0
31	39.60726512820815	41.0	40.0	41.0	37.0	41.0
32	39.537922728048144	41.0	40.0	41.0	37.0	41.0
33	39.44055924516315	41.0	40.0	41.0	36.0	41.0
34	39.39628885607785	41.0	39.0	41.0	36.0	41.0
35	39.35172189313145	41.0	39.0	41.0	36.0	41.0
36	39.328952863874065	41.0	39.0	41.0	35.0	41.0
37	39.229601600812565	41.0	39.0	41.0	35.0	41.0
38	39.19367351580714	41.0	39.0	41.0	35.0	41.0
39	39.12004623611005	41.0	39.0	41.0	35.0	41.0
40	39.06046283169703	41.0	39.0	41.0	35.0	41.0
41	38.9678048240067	41.0	39.0	41.0	35.0	41.0
42	38.85922202548672	41.0	39.0	41.0	35.0	41.0
43	37.657448366735885	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	2.0
15	6.0
16	9.0
17	15.0
18	20.0
19	41.0
20	85.0
21	199.0
22	330.0
23	640.0
24	1004.0
25	1513.0
26	2173.0
27	2878.0
28	4211.0
29	5440.0
30	7300.0
31	9441.0
32	12231.0
33	16592.0
34	24983.0
35	40789.0
36	69549.0
37	139803.0
38	364597.0
39	1336109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59467716096951	15.261151667382366	11.164853889578444	27.979317282069683
2	19.560688325872416	18.108162693067133	33.208674270722426	29.122474710338025
3	20.288152143861364	18.849695386781335	28.59329870527127	32.26885376408603
4	15.504244192544576	13.418276008792313	31.198246635724946	39.87923316293817
5	17.456974730926625	33.47215931261532	32.40959154181152	16.66127441464653
6	39.71070077707254	30.863779949057925	13.502493181252218	15.92302609261732
7	33.27852844461961	28.124123759046725	18.262283059897136	20.335064736436525
8	29.793859107317576	31.952230529558367	17.860364202505536	20.39354616061852
9	27.96284640317182	12.726253992717517	16.64264663494062	42.66825296917005
10	17.427072242451338	24.6661215590079	31.599038022239608	26.30776817630115
11	39.4427548721448	18.64885850926781	19.512844344311958	22.395542274275428
12	23.800420007411898	22.440935232190373	27.575927810490775	26.182716949906958
13	34.05839514814967	17.146528075985653	21.640921114294173	27.1541556615705
14	24.15606353837617	17.75849965979792	23.267518446403955	34.81791835542196
15	28.30505832455867	24.578031769188083	19.78681976740766	27.330090138845588
16	27.761225198091733	23.118447188283714	21.853915069089457	27.2664125445351
17	27.130576814100642	23.35124541413476	22.82447141224061	26.693706359523993
18	27.264451725618688	22.77844118817783	23.068446305915202	26.88866078028828
19	28.111721579400424	22.7849609110749	22.97221911759227	26.131098391932404
20	27.66955691374946	22.370443792145352	22.80084354429784	27.15915574980735
21	27.29214829281301	22.998690172963837	22.80917702469259	26.89998450953056
22	27.976572135586707	22.545790023745518	22.056712765519393	27.420925075148382
23	27.239353243488612	22.445003931441928	22.52711322356669	27.78852960150277
24	27.262196783864812	22.5204954597238	22.52529946606901	27.69200829034238
25	27.38822841971721	22.02568280616717	22.49647542799775	28.089613346117876
26	26.882092036918298	22.55780003960854	23.06472074997402	27.495387173499143
27	27.621173706986983	22.595202660439107	22.674909949391264	27.108713683182646
28	27.16538134986696	22.944473529925038	22.59770270455753	27.29244241565047
29	27.104497922512362	22.96192481828111	22.82770676345269	27.105870495753848
30	27.082928914431825	22.88231557027477	22.817118341304063	27.217637173989345
31	27.111703932030174	22.251127961081664	22.798735663962695	27.838432442925466
32	26.428456580606323	22.586035832004878	23.082613222586282	27.902894364802517
33	26.860621069783587	22.162547966532742	23.23614534374136	27.74068561994231
34	26.977534897674666	22.088625093384	23.696447584369135	27.2373924245722
35	26.59399871762443	22.16097931139961	23.781154961558144	27.463867009417815
36	26.382083213233177	21.629156200795702	24.34415509293301	27.64460549303811
37	25.957958081613203	21.285228562856993	24.43013700241769	28.326676353112113
38	25.575990556696098	20.972330884270505	25.450105982262432	28.00157257677096
39	25.182356159226337	20.729189338635386	26.223207860530874	27.8652466416074
40	25.257357482779106	20.78247459268889	26.46458466914122	27.49558325539078
41	24.810437831255847	20.661590106492074	27.12719440146983	27.40077766078225
42	24.21219197985847	20.938702839854038	27.15949889311772	27.68960628716977
43	23.846940436203777	20.570657129243457	27.294403234566882	28.28799919998588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	29.0
2	35.0
3	176.0
4	317.0
5	317.0
6	434.5
7	552.0
8	535.5
9	519.0
10	663.0
11	807.0
12	807.0
13	1231.0
14	1655.0
15	2646.0
16	3637.0
17	3130.5
18	2624.0
19	2624.0
20	2854.5
21	3085.0
22	2855.0
23	2625.0
24	3389.0
25	4153.0
26	4153.0
27	5479.0
28	6805.0
29	8309.0
30	9813.0
31	12472.5
32	15132.0
33	15132.0
34	19240.5
35	23349.0
36	29381.5
37	35414.0
38	43568.0
39	51722.0
40	51722.0
41	61973.5
42	72225.0
43	85554.0
44	98883.0
45	114376.5
46	129870.0
47	129870.0
48	148449.0
49	167028.0
50	180796.5
51	194565.0
52	201593.5
53	208622.0
54	208622.0
55	206188.0
56	203754.0
57	194122.5
58	184491.0
59	173176.5
60	161862.0
61	161862.0
62	146831.0
63	131800.0
64	114341.5
65	96883.0
66	84362.0
67	71841.0
68	71841.0
69	61238.5
70	50636.0
71	43571.0
72	36506.0
73	30814.5
74	25123.0
75	25123.0
76	21226.0
77	17329.0
78	14431.0
79	11533.0
80	9442.5
81	7352.0
82	7352.0
83	5741.0
84	4130.0
85	3170.5
86	2211.0
87	1483.0
88	755.0
89	755.0
90	488.0
91	221.0
92	138.5
93	56.0
94	35.5
95	15.0
96	15.0
97	10.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2039964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.20554647947682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.44485242179347	55.90717601963608
2	8.419289175415022	11.148072816541905
3	2.732393456588771	5.426988059712186
4	1.2760795778720313	3.379341832172718
5	0.7689698615063919	2.545503495363914
6	0.505460117322942	2.0078557974567506
7	0.33892873286173997	1.5707273383695672
8	0.2502619552419889	1.3254983607854594
9	0.19372650078220516	1.1543191966658417
>10	1.0126943119482872	11.700084411823308
>50	0.042390331739711844	1.8954505656108007
>100	0.014210308387906805	1.501382692091264
>500	5.94598831632592E-4	0.2791031007426449
>1k	1.4864970790814594E-4	0.15849631302751224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.902047291030626E-5
2	0.0	0.0	0.0	0.0	4.902047291030626E-5
3	0.0	0.0	0.0	0.0	4.902047291030626E-5
4	0.0	0.0	0.0	0.0	4.902047291030626E-5
5	0.0	4.902047291030626E-5	0.0	0.0	4.902047291030626E-5
6	0.0	4.902047291030626E-5	0.0	0.0	1.9608189164122504E-4
7	0.0	4.902047291030626E-5	0.0	0.0	1.9608189164122504E-4
8	0.0	4.902047291030626E-5	0.0	0.0	1.9608189164122504E-4
9	0.0	4.902047291030626E-5	0.0	4.902047291030626E-5	2.451023645515313E-4
10	0.0	4.902047291030626E-5	0.0	4.902047291030626E-5	2.451023645515313E-4
11	0.0	4.902047291030626E-5	0.0	4.902047291030626E-5	2.941228374618376E-4
12	0.0	4.902047291030626E-5	0.0	1.470614187309188E-4	4.4118425619275636E-4
13	0.0	4.902047291030626E-5	0.0	1.470614187309188E-4	5.392252020133689E-4
14	0.0	4.902047291030626E-5	0.0	2.451023645515313E-4	7.843275665649002E-4
15	0.0	4.902047291030626E-5	0.0	5.392252020133689E-4	8.333480394752064E-4
16	0.0	4.902047291030626E-5	0.0	8.333480394752064E-4	8.823685123855127E-4
17	0.0	4.902047291030626E-5	0.0	0.0017647370247710255	8.823685123855127E-4
18	0.0	4.902047291030626E-5	0.0	0.0021569008080534755	9.313889852958189E-4
19	4.902047291030626E-5	4.902047291030626E-5	0.0	0.003039269320438988	9.313889852958189E-4
20	4.902047291030626E-5	9.804094582061252E-5	0.0	0.004509883507748176	9.804094582061252E-4
21	4.902047291030626E-5	9.804094582061252E-5	0.0	0.007500132355276858	0.0010784504040267377
22	4.902047291030626E-5	9.804094582061252E-5	0.0	0.011715893025563195	0.001127470876937044
23	4.902047291030626E-5	9.804094582061252E-5	0.0	0.014657121400181572	0.0011764913498473503
24	4.902047291030626E-5	9.804094582061252E-5	0.0	0.021176844297252303	0.0011764913498473503
25	4.902047291030626E-5	9.804094582061252E-5	0.0	0.026618116790296298	0.0011764913498473503
26	4.902047291030626E-5	9.804094582061252E-5	0.0	0.034118249145573154	0.0011764913498473503
27	4.902047291030626E-5	9.804094582061252E-5	0.0	0.056569625738493425	0.0012255118227576566
28	4.902047291030626E-5	9.804094582061252E-5	0.0	0.17343443315666354	0.0012745322956679629
29	4.902047291030626E-5	9.804094582061252E-5	0.0	0.3704967342560947	0.0012745322956679629
30	4.902047291030626E-5	1.470614187309188E-4	0.0	0.6158442011721775	0.0012745322956679629
31	4.902047291030626E-5	1.470614187309188E-4	0.0	1.2536495742081724	0.0012745322956679629
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGT	125	4.129106E-10	19.240002	4
ATAAGAC	130	1.3948011E-8	17.076925	3
CTTATAC	2710	0.0	17.066422	37
TATAGAT	120	1.0423355E-7	16.958334	2
GTATAGA	145	2.9831426E-9	16.586206	1
TATACAC	980	0.0	16.42347	37
ATACACA	725	0.0	15.310345	37
GTAGTAC	210	9.094947E-12	14.97619	3
CGTCTAT	75	0.004105856	14.8	1
ATTGGAC	150	8.110328E-8	14.8	3
ACGATCG	80	0.006300828	13.875	5
TAACCGA	80	0.006300828	13.875	5
GTATAAA	160	1.7852835E-7	13.875	1
TCTATAT	80	0.006300828	13.875	3
TATACTG	175	3.5697667E-8	13.742858	5
TACTTAC	165	2.597717E-7	13.454545	31
TATATGG	110	2.4578228E-4	13.454545	2
GTATATG	165	2.597717E-7	13.454545	1
CTATTAC	110	2.4578228E-4	13.454545	1
CCCTATA	195	1.0270014E-8	13.282052	2
>>END_MODULE