##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727606.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3370481
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.121889427651425	34.0	33.0	34.0	31.0	34.0
2	33.29307359988085	34.0	33.0	34.0	31.0	34.0
3	33.383771930475206	34.0	34.0	34.0	31.0	34.0
4	36.57784541731581	37.0	37.0	37.0	35.0	37.0
5	36.566510239933116	37.0	37.0	37.0	35.0	37.0
6	36.61462325406967	37.0	37.0	37.0	35.0	37.0
7	36.619763469961704	37.0	37.0	37.0	35.0	37.0
8	36.60418646478055	37.0	37.0	37.0	35.0	37.0
9	38.25668443168794	39.0	39.0	39.0	37.0	39.0
10	38.37892603459269	39.0	39.0	39.0	37.0	39.0
11	38.47506720850822	39.0	39.0	39.0	37.0	39.0
12	38.463982440488465	39.0	39.0	39.0	37.0	39.0
13	38.49535363053523	39.0	39.0	39.0	37.0	39.0
14	40.042376147499425	41.0	40.0	41.0	38.0	41.0
15	40.06789387034076	41.0	40.0	41.0	38.0	41.0
16	40.052658062751284	41.0	40.0	41.0	38.0	41.0
17	40.06176863183623	41.0	40.0	41.0	38.0	41.0
18	40.05971462233432	41.0	40.0	41.0	38.0	41.0
19	40.101209886660094	41.0	40.0	41.0	38.0	41.0
20	40.08103798834647	41.0	40.0	41.0	38.0	41.0
21	40.03381090117404	41.0	40.0	41.0	38.0	41.0
22	40.001328000365525	41.0	40.0	41.0	38.0	41.0
23	39.94467644232381	41.0	40.0	41.0	38.0	41.0
24	39.94742234120293	41.0	40.0	41.0	38.0	41.0
25	39.90647180624961	41.0	40.0	41.0	38.0	41.0
26	39.819892769014274	41.0	40.0	41.0	38.0	41.0
27	39.779735889328556	41.0	40.0	41.0	37.0	41.0
28	39.72640373881354	41.0	40.0	41.0	37.0	41.0
29	39.63546953684059	41.0	40.0	41.0	37.0	41.0
30	39.51959853801282	41.0	40.0	41.0	36.0	41.0
31	39.42341167328936	41.0	39.0	41.0	35.0	41.0
32	39.319075229915256	41.0	39.0	41.0	35.0	41.0
33	39.18620932739274	41.0	39.0	41.0	35.0	41.0
34	39.107068100962444	41.0	39.0	41.0	35.0	41.0
35	39.017214160234104	41.0	39.0	41.0	35.0	41.0
36	38.93457729030367	41.0	39.0	41.0	35.0	41.0
37	38.79960486351948	41.0	39.0	41.0	35.0	41.0
38	38.730954721299426	41.0	39.0	41.0	35.0	41.0
39	38.62246545819425	41.0	39.0	41.0	35.0	41.0
40	38.5222331768077	41.0	38.0	41.0	35.0	41.0
41	38.38463708889028	40.0	38.0	41.0	35.0	41.0
42	38.21233497533438	40.0	38.0	41.0	35.0	41.0
43	37.04269924678407	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	6.0
12	6.0
13	4.0
14	5.0
15	4.0
16	11.0
17	27.0
18	32.0
19	68.0
20	166.0
21	307.0
22	646.0
23	1099.0
24	1833.0
25	2946.0
26	4377.0
27	6571.0
28	10029.0
29	14508.0
30	19513.0
31	22607.0
32	26238.0
33	32851.0
34	47386.0
35	70589.0
36	118374.0
37	273470.0
38	594251.0
39	2122549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72795396265399	16.938680265516997	12.341947632993628	24.991418138835378
2	18.200458628901927	20.545672857968935	34.8187098517986	26.435158661330533
3	20.69707558060704	22.168942652398872	29.347146594210145	27.78683517278394
4	14.8978439575835	14.443517112245996	32.32467413404793	38.33396479612257
5	16.686639088011475	34.77607498751662	32.5385011812854	15.998784743186507
6	36.943658783419934	32.17241100009168	14.147594957514967	16.736335258973423
7	30.420435540209244	29.30290958471506	20.182460604287638	20.09419427078806
8	28.667421652873877	33.39194020082	19.133441191331446	18.807196954974675
9	26.652842724821767	14.401356957656786	19.005061888792728	39.940738428728714
10	17.263500372795455	26.003143171553262	32.30868235127271	24.424674104378575
11	35.6086267805693	20.681291483322408	20.94496304830082	22.76511868780747
12	22.354168440646898	25.068677141333833	28.634726022784285	23.942428395234984
13	33.172921016317844	18.534238881631435	23.437337282126794	24.85550281992392
14	22.838876706321738	19.8882592721929	26.459546871796636	30.813317149688725
15	27.677444257956058	26.126656699741073	22.110345674697466	24.085553367605396
16	25.121725949500977	24.22455429951986	25.145728458341704	25.50799129263746
17	24.847610771281605	25.1066539167555	26.136210232308088	23.909525079654802
18	24.911043853978114	24.15951907161025	26.63050169990574	24.298935374505895
19	25.273277018918073	25.066362931581576	26.545261640697575	23.11509840880278
20	25.0203754300944	24.124212538210422	26.11469994935441	24.74071208234077
21	24.157086184434803	25.647526272956295	26.264708212269998	23.930679330338904
22	24.838531948407365	25.129885022345476	25.249274510077345	24.782308519169817
23	23.964502395948827	24.51955670422115	25.87152990923254	25.644410990597482
24	24.505078058591636	24.897128926108767	25.610736271766555	24.987056743533046
25	24.47543837214926	24.166610047646017	25.36955407848316	25.988397501721565
26	24.090300464533104	24.892708192094837	26.456846960419	24.560144382953055
27	26.00628812326787	24.083446843343726	25.832989416050705	24.077275617337705
28	24.333203480452788	25.25405127636085	26.282836188662685	24.129909054523672
29	24.821917109160385	24.894191659884747	26.45705464590959	23.82683658504528
30	23.80241870522338	25.136234264486284	26.946421000444744	24.11492602984559
31	24.012893115255658	24.66612332186415	26.481294509596705	24.839689053283493
32	23.570819713862797	24.376995449610902	26.349592239208587	25.702592597317715
33	24.2287673480432	24.587529198354773	26.322711802855437	24.860991650746588
34	24.791891721092625	24.54169004364659	26.058891891098035	24.607526344162746
35	24.010816260349785	24.60518246505469	26.682927451601124	24.701073822994406
36	23.548241334100382	24.58174367397413	27.216412138208167	24.65360285371732
37	23.35218623098602	24.203103355277776	26.6799901853771	25.7647202283591
38	23.184049991677746	23.771384559058482	27.830122762893488	25.214442686370287
39	22.712663266756287	23.33806361762609	28.34571682795423	25.60355628766339
40	23.08305550454075	23.70712073439963	28.45409898468498	24.75572477637465
41	22.874717288125936	23.24522820333359	28.933793129229922	24.94626137931055
42	22.05397389868093	23.770405470317144	28.97423246118284	25.201388169819083
43	22.219261879832583	23.46285292811323	28.915635483481438	25.402249708572754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	540.0
2	659.0
3	2545.0
4	4431.0
5	4431.0
6	5877.5
7	7324.0
8	7253.5
9	7183.0
10	9302.0
11	11421.0
12	11421.0
13	17326.5
14	23232.0
15	35550.5
16	47869.0
17	42811.5
18	37754.0
19	37754.0
20	39803.0
21	41852.0
22	36220.0
23	30588.0
24	33264.0
25	35940.0
26	35940.0
27	39795.0
28	43650.0
29	47690.0
30	51730.0
31	55760.5
32	59791.0
33	59791.0
34	65542.5
35	71294.0
36	79380.5
37	87467.0
38	98825.0
39	110183.0
40	110183.0
41	123135.0
42	136087.0
43	150964.5
44	165842.0
45	187730.5
46	209619.0
47	209619.0
48	240524.0
49	271429.0
50	295435.5
51	319442.0
52	328703.0
53	337964.0
54	337964.0
55	323132.5
56	308301.0
57	265560.5
58	222820.0
59	203723.5
60	184627.0
61	184627.0
62	164568.0
63	144509.0
64	125107.5
65	105706.0
66	93302.0
67	80898.0
68	80898.0
69	71433.0
70	61968.0
71	56120.5
72	50273.0
73	43013.5
74	35754.0
75	35754.0
76	30651.0
77	25548.0
78	21256.0
79	16964.0
80	13516.5
81	10069.0
82	10069.0
83	7807.5
84	5546.0
85	4350.0
86	3154.0
87	2013.5
88	873.0
89	873.0
90	552.5
91	232.0
92	137.0
93	42.0
94	28.0
95	14.0
96	14.0
97	10.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3370481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.449611199548215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.12735161313887	49.75660604235201
2	8.045520962377108	9.405151442975137
3	2.5142042821769777	4.408627883084507
4	1.1963150256576613	2.7969659248747143
5	0.7105874367455768	2.0766779700531264
6	0.4579983504805237	1.6061895309372618
7	0.31902538240091	1.3052836694884267
8	0.23614283049298723	1.1041965303900738
9	0.19859909154853903	1.044723571703603
>10	1.054860954329526	11.315305659115268
>50	0.08372080016129206	3.4005650959245326
>100	0.049457696707104745	5.367320586477106
>500	0.003260628870648109	1.3459590551885945
>1k	0.002598313629700881	2.816749639825748
>5k	2.0378930429026517E-4	0.805002569123686
>10k+	1.5284197821769887E-4	1.444674828486165
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23761	0.7049735631205161	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13369	0.39664961766584644	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11384	0.33775594640646245	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	9243	0.2742338556425626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7124	0.21136449070622265	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	5416	0.160689231002934	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	5250	0.1557641179404364	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	4272	0.12674748796981794	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	4099	0.1216146894167331	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA	4050	0.12016089098262235	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG	3404	0.10099448713699914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.933871159635672E-5
2	0.0	0.0	0.0	0.0	5.933871159635672E-5
3	0.0	0.0	0.0	0.0	5.933871159635672E-5
4	0.0	0.0	0.0	0.0	5.933871159635672E-5
5	0.0	0.0	0.0	0.0	5.933871159635672E-5
6	0.0	0.0	0.0	0.0	8.900806739453509E-5
7	0.0	0.0	0.0	0.0	8.900806739453509E-5
8	0.0	0.0	0.0	0.0	8.900806739453509E-5
9	0.0	0.0	0.0	0.0	1.1867742319271345E-4
10	5.933871159635672E-5	0.0	0.0	2.0768549058724852E-4	1.1867742319271345E-4
11	5.933871159635672E-5	0.0	0.0	5.043790485690322E-4	1.1867742319271345E-4
12	5.933871159635672E-5	0.0	0.0	0.0010977661645325994	1.7801613478907018E-4
13	5.933871159635672E-5	0.0	0.0	0.0013647903667162046	1.7801613478907018E-4
14	5.933871159635672E-5	0.0	0.0	0.001720822636294345	2.6702420218360524E-4
15	5.933871159635672E-5	0.0	0.0	0.002966935579817836	3.8570162537631867E-4
16	5.933871159635672E-5	0.0	0.0	0.005103129197286678	3.8570162537631867E-4
17	5.933871159635672E-5	0.0	0.0	0.008396427690884476	4.1537098117449704E-4
18	5.933871159635672E-5	0.0	0.0	0.010057911615582464	4.1537098117449704E-4
19	8.900806739453509E-5	0.0	0.0	0.014448976273712862	4.450403369726754E-4
20	1.1867742319271345E-4	0.0	0.0	0.020293839365953997	5.340484043672105E-4
21	1.1867742319271345E-4	0.0	0.0	0.03020340420254557	5.340484043672105E-4
22	1.1867742319271345E-4	0.0	0.0	0.047767662835067164	5.340484043672105E-4
23	1.1867742319271345E-4	0.0	0.0	0.06111887294424742	5.340484043672105E-4
24	1.1867742319271345E-4	0.0	0.0	0.08678286570967171	5.340484043672105E-4
25	1.1867742319271345E-4	0.0	0.0	0.1085898422213328	5.340484043672105E-4
26	1.1867742319271345E-4	0.0	0.0	0.14000969001160368	5.637177601653889E-4
27	1.1867742319271345E-4	0.0	0.0	0.17558324761361954	5.933871159635672E-4
28	1.1867742319271345E-4	0.0	0.0	0.2930145578628095	5.933871159635672E-4
29	1.483467789908918E-4	0.0	0.0	0.4814446365370403	5.933871159635672E-4
30	1.483467789908918E-4	0.0	0.0	0.7086228938836919	5.933871159635672E-4
31	1.483467789908918E-4	0.0	0.0	1.2251663783299773	5.933871159635672E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	380	0.0	17.039474	9
GGACCGT	510	0.0	16.686274	6
CTAACGG	120	1.938104E-6	15.416667	10
TAGATCG	160	1.0986696E-8	15.03125	5
ACCGTTA	445	0.0	14.966293	8
GACCGTT	465	0.0	14.720429	7
TTAGGAC	715	0.0	14.489511	3
TATCACG	225	2.7284841E-11	13.9777775	2
TAATCCC	505	0.0	13.920793	5
CTTATAC	4245	0.0	13.815077	37
TAGGACC	820	0.0	13.762195	4
AAACCGC	125	4.849811E-5	13.320001	6
CGGTATA	125	4.849811E-5	13.320001	14
CGTTATT	300	0.0	12.95	10
CATATAC	330	0.0	12.893939	3
TATACAC	2060	0.0	12.842233	37
TCGGGTA	305	0.0	12.737705	25
GTCTTAT	310	0.0	12.532257	1
GTCCTAA	635	0.0	12.527559	1
ATTGGAC	345	0.0	12.333334	3
>>END_MODULE