##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727605.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2001963
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13726827119183	34.0	33.0	34.0	31.0	34.0
2	33.31252425744132	34.0	33.0	34.0	31.0	34.0
3	33.395250062064086	34.0	34.0	34.0	31.0	34.0
4	36.589967446950816	37.0	37.0	37.0	35.0	37.0
5	36.57043012283444	37.0	37.0	37.0	35.0	37.0
6	36.61316867494554	37.0	37.0	37.0	35.0	37.0
7	36.61510027907609	37.0	37.0	37.0	35.0	37.0
8	36.5957982240431	37.0	37.0	37.0	35.0	37.0
9	38.26006075037351	39.0	39.0	39.0	37.0	39.0
10	38.36962021775627	39.0	39.0	39.0	37.0	39.0
11	38.471582641637234	39.0	39.0	39.0	37.0	39.0
12	38.451280068612654	39.0	39.0	39.0	37.0	39.0
13	38.48437508585324	39.0	39.0	39.0	37.0	39.0
14	40.039188036941745	41.0	40.0	41.0	38.0	41.0
15	40.063637040245	41.0	40.0	41.0	38.0	41.0
16	40.04992050302628	41.0	40.0	41.0	38.0	41.0
17	40.049888034893755	41.0	40.0	41.0	38.0	41.0
18	40.03824995766655	41.0	40.0	41.0	38.0	41.0
19	40.066522707962136	41.0	40.0	41.0	38.0	41.0
20	40.03782137831718	41.0	40.0	41.0	38.0	41.0
21	39.98940439958181	41.0	40.0	41.0	38.0	41.0
22	39.96489595462054	41.0	40.0	41.0	38.0	41.0
23	39.90677899641502	41.0	40.0	41.0	38.0	41.0
24	39.90105161783709	41.0	40.0	41.0	38.0	41.0
25	39.85622111897173	41.0	40.0	41.0	38.0	41.0
26	39.783756742756985	41.0	40.0	41.0	38.0	41.0
27	39.74451026317669	41.0	40.0	41.0	37.0	41.0
28	39.679714859865044	41.0	40.0	41.0	37.0	41.0
29	39.60376740229465	41.0	40.0	41.0	37.0	41.0
30	39.50550784405107	41.0	40.0	41.0	36.0	41.0
31	39.430506957421294	41.0	39.0	41.0	36.0	41.0
32	39.3334352333185	41.0	39.0	41.0	35.0	41.0
33	39.21501746036265	41.0	39.0	41.0	35.0	41.0
34	39.136907125656165	41.0	39.0	41.0	35.0	41.0
35	39.055442083594954	41.0	39.0	41.0	35.0	41.0
36	39.010674023445986	41.0	39.0	41.0	35.0	41.0
37	38.8858465416194	41.0	39.0	41.0	35.0	41.0
38	38.82790990642684	41.0	39.0	41.0	35.0	41.0
39	38.73013537213225	41.0	38.0	41.0	35.0	41.0
40	38.63854326978071	40.0	38.0	41.0	35.0	41.0
41	38.52051461490547	40.0	38.0	41.0	35.0	41.0
42	38.368325988042734	40.0	38.0	41.0	35.0	41.0
43	37.15526061170961	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	3.0
15	2.0
16	6.0
17	12.0
18	19.0
19	60.0
20	109.0
21	172.0
22	376.0
23	674.0
24	1159.0
25	1702.0
26	2553.0
27	3482.0
28	5059.0
29	6894.0
30	9040.0
31	11202.0
32	14004.0
33	18363.0
34	28645.0
35	46169.0
36	81254.0
37	161993.0
38	364346.0
39	1244662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.598894684866806	16.139159415034143	11.556357435177373	26.705588464921682
2	20.07609531245083	19.21044494828326	33.01000068432833	27.70345905493758
3	20.78300148404341	20.26436053013967	28.742289442911783	30.21034854290514
4	15.399635257994278	13.71933447321454	31.134091888811135	39.74693837998005
5	17.743085161913584	33.36045671173743	31.878561192189864	17.017896934159126
6	38.65436074492885	31.19133570400652	13.28036532143701	16.87393822962762
7	32.16702806195719	28.437838261746094	18.879769506229636	20.51536417006708
8	29.71943037908293	31.327152399919477	17.99953345791106	20.95388376308653
9	27.733079981997673	13.245149885387491	17.506467402244695	41.51530273037014
10	18.037346344562813	24.491361728463513	30.61130500413844	26.859986922835237
11	38.33247667414433	19.1839209815566	19.44521452194671	23.03838782235236
12	23.871769857884487	23.509925008604053	26.882165154900466	25.736139978610993
13	34.32421078711245	17.356514580938807	22.039967771632142	26.2793068603166
14	23.779660263451422	19.096207072758087	23.94659641561807	33.177536248172416
15	28.678252295372094	24.810798201565166	20.248076512902585	26.262872990160158
16	27.187415551636068	23.3386930727491	22.521994662239013	26.951896713375824
17	27.138113941166747	23.685452728147325	23.257972300187365	25.918461030498563
18	26.986562688721023	22.334928267905052	23.97661695046312	26.701892092910807
19	27.83977526058174	23.172805891017966	23.71192674390086	25.275492104499435
20	26.858638246561	22.641827046753612	23.641096264016866	26.85843844266852
21	26.37756042444341	23.685003169389244	23.825615158721714	26.111821247445633
22	27.323332149495272	23.476957366344934	22.37893507522367	26.820775408936132
23	26.463975607940803	22.533683189949063	23.331600034566076	27.670741167544055
24	26.396292039363367	23.476457856613735	23.04682953680962	27.08042056721328
25	26.552238977443636	22.486479520350773	23.143984179527795	27.817297322677792
26	26.07181051797661	23.195283828921916	23.73460448569729	26.998301167404193
27	27.661300433624397	22.867405641363003	23.517467605545157	25.953826319467442
28	26.525015697093302	23.203176082674855	23.616620287188127	26.655187933043717
29	26.681462144904778	23.019506354513044	23.557628187933545	26.741403312648636
30	25.67764738908761	23.4826517772806	24.15928765916253	26.68041317446926
31	26.46737227411296	22.27313891415576	23.64404337143094	27.615445440300345
32	25.52959270476028	22.894978578525176	23.705433117395277	27.86999559931927
33	26.406981547611018	22.376737232406395	24.2343140207886	26.98196719919399
34	26.660632589113785	22.67599351236761	24.125221095494773	26.538152803023834
35	25.985095628640487	23.040236008357798	24.380270764244894	26.59439759875682
36	25.699226209475402	22.25455715215516	24.934376909063754	27.111839729305686
37	25.58853485304174	22.319193711372286	24.42492693421407	27.667344501371904
38	25.26425313554746	21.77647638842476	25.835392562200198	27.123877913827577
39	25.11879590182236	21.328715865378133	26.077405026966034	27.47508320583347
40	24.976585481350057	21.523274905680076	26.710983170018626	26.789156442951246
41	24.89281769942801	20.897389212487944	27.295759212333092	26.91403387575095
42	24.031313266029393	21.423972371117745	27.24211186720234	27.302602495650518
43	23.77186791164472	21.045893455573356	27.53662280471717	27.645615828064756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	145.5
2	146.0
3	648.5
4	1151.0
5	1151.0
6	1512.0
7	1873.0
8	1820.0
9	1767.0
10	2228.0
11	2689.0
12	2689.0
13	4238.5
14	5788.0
15	8949.5
16	12111.0
17	10506.5
18	8902.0
19	8902.0
20	9322.5
21	9743.0
22	8468.0
23	7193.0
24	8575.5
25	9958.0
26	9958.0
27	11922.0
28	13886.0
29	15970.5
30	18055.0
31	20865.5
32	23676.0
33	23676.0
34	27328.0
35	30980.0
36	36101.0
37	41222.0
38	48324.0
39	55426.0
40	55426.0
41	65031.5
42	74637.0
43	84290.5
44	93944.0
45	106995.0
46	120046.0
47	120046.0
48	139812.5
49	159579.0
50	169768.5
51	179958.0
52	186613.0
53	193268.0
54	193268.0
55	188262.5
56	183257.0
57	170489.0
58	157721.0
59	146950.0
60	136179.0
61	136179.0
62	123403.5
63	110628.0
64	97588.0
65	84548.0
66	74879.0
67	65210.0
68	65210.0
69	56970.5
70	48731.0
71	43513.0
72	38295.0
73	34217.5
74	30140.0
75	30140.0
76	27547.5
77	24955.0
78	22810.5
79	20666.0
80	18164.0
81	15662.0
82	15662.0
83	13116.5
84	10571.0
85	8203.0
86	5835.0
87	4232.0
88	2629.0
89	2629.0
90	1634.5
91	640.0
92	364.5
93	89.0
94	66.5
95	44.0
96	44.0
97	29.5
98	15.0
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2001963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.639125225883866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41713530116236	44.74422166403545
2	8.323044807535544	8.928816853840829
3	2.7347539461111348	4.400694281323056
4	1.4249370691982832	3.057295115749226
5	0.8647568052749358	2.319239928403878
6	0.5950438769824042	1.9150579819412767
7	0.4214789779515708	1.5825434574895296
8	0.3082220653632184	1.3226209569118585
9	0.2510586770331971	1.2119911034785555
>10	1.4718000051729654	14.543005106965005
>50	0.10527898584684241	3.9466436986290336
>100	0.07461680135389227	7.566241232739459
>500	0.0055296211882871205	2.0594374984245745
>1k	0.002249337432523574	2.075775856745816
>5k	9.37223930218156E-5	0.3264152633225299
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6493	0.32433166846739925	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	3965	0.19805560842033545	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3371	0.16838473038712504	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2950	0.14735537070365437	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	2301	0.1149371891488504	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2133	0.10654542566471009	No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCT	2006	0.10020165207848496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.995097311988283E-5
2	0.0	0.0	0.0	0.0	4.995097311988283E-5
3	0.0	0.0	0.0	0.0	4.995097311988283E-5
4	0.0	0.0	0.0	0.0	4.995097311988283E-5
5	0.0	4.995097311988283E-5	0.0	0.0	4.995097311988283E-5
6	4.995097311988283E-5	4.995097311988283E-5	0.0	0.0	9.990194623976566E-5
7	4.995097311988283E-5	4.995097311988283E-5	0.0	0.0	9.990194623976566E-5
8	9.990194623976566E-5	4.995097311988283E-5	0.0	0.0	9.990194623976566E-5
9	9.990194623976566E-5	4.995097311988283E-5	0.0	4.995097311988283E-5	9.990194623976566E-5
10	1.9980389247953133E-4	4.995097311988283E-5	0.0	1.498529193596485E-4	1.498529193596485E-4
11	1.9980389247953133E-4	4.995097311988283E-5	0.0	3.9960778495906266E-4	1.498529193596485E-4
12	1.9980389247953133E-4	4.995097311988283E-5	0.0	9.490684892777739E-4	1.498529193596485E-4
13	2.497548655994142E-4	4.995097311988283E-5	0.0	0.0010489704355175395	1.498529193596485E-4
14	2.497548655994142E-4	4.995097311988283E-5	0.0	0.0013986272473567195	1.498529193596485E-4
15	2.497548655994142E-4	4.995097311988283E-5	0.0	0.002147891844154962	2.497548655994142E-4
16	2.497548655994142E-4	4.995097311988283E-5	0.0	0.0037962739571110954	2.99705838719297E-4
17	2.497548655994142E-4	4.995097311988283E-5	0.0	0.00599411677438594	2.99705838719297E-4
18	2.497548655994142E-4	4.995097311988283E-5	0.0	0.007542596941102308	3.4965681183917987E-4
19	2.497548655994142E-4	4.995097311988283E-5	0.0	0.010839361167014575	3.4965681183917987E-4
20	2.497548655994142E-4	4.995097311988283E-5	0.0	0.015584703613403444	3.9960778495906266E-4
21	2.497548655994142E-4	4.995097311988283E-5	0.0	0.02392651612442388	3.9960778495906266E-4
22	2.497548655994142E-4	4.995097311988283E-5	0.0	0.03836234735607002	3.9960778495906266E-4
23	2.497548655994142E-4	4.995097311988283E-5	0.0	0.049801120200523186	4.995097311988284E-4
24	2.497548655994142E-4	4.995097311988283E-5	0.0	0.0715797444807921	4.995097311988284E-4
25	2.497548655994142E-4	4.995097311988283E-5	0.0	0.08566591890059906	4.995097311988284E-4
26	2.497548655994142E-4	4.995097311988283E-5	0.0	0.10894307237446446	4.995097311988284E-4
27	2.497548655994142E-4	4.995097311988283E-5	0.0	0.14360904771966315	4.995097311988284E-4
28	2.497548655994142E-4	4.995097311988283E-5	0.0	0.26758736300321234	4.995097311988284E-4
29	2.497548655994142E-4	4.995097311988283E-5	0.0	0.49026880117165	4.995097311988284E-4
30	2.497548655994142E-4	4.995097311988283E-5	0.0	0.7588052326641401	4.995097311988284E-4
31	2.497548655994142E-4	4.995097311988283E-5	0.0	1.3040201042676614	4.995097311988284E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGAG	105	9.349944E-6	15.857142	2
GTATACA	145	5.351467E-8	15.310345	1
TATACAC	1145	0.0	15.026201	37
TATACTA	100	1.0940077E-4	14.799999	5
CTAGACA	180	3.3342076E-9	14.388889	4
TAAGGTA	90	8.2777935E-4	14.388889	5
GGCCTAT	220	1.8189894E-11	14.295454	1
TAATCGA	610	0.0	14.254099	20
TAGACAG	195	6.730261E-10	14.23077	5
GTAAGGT	195	6.730261E-10	14.23077	4
TAAACCG	80	0.0063007735	13.875	5
TATAACG	80	0.0063007735	13.875	2
TAATACT	255	1.8189894E-12	13.784313	4
TTAAGAC	215	1.9826984E-10	13.767442	3
CTTATAC	2660	0.0	13.701127	37
TAATACC	95	0.0012456384	13.631579	4
TAACTTA	125	4.847889E-5	13.320001	5
CTAATCG	640	0.0	13.296875	19
TACCGTT	195	1.0270014E-8	13.282052	18
GACTAAT	655	0.0	13.27481	17
>>END_MODULE