FastQCFastQC Report
Thu 9 Feb 2017
SRR2727604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3373461
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52230.15482615628282054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC36550.016.80437937
CTATACG904.4500608E-516.44444535
TATACCG1302.593115E-715.6538465
ACCGCTA4750.014.8000018
TCTATAC1904.5656634E-1014.6052643
CCGCTAC5350.014.1775699
CGGTTAC800.00630195513.87528
CGCTACG4750.013.6315810
CCCTATA6650.013.6315782
ATTATAC4350.013.6091963
TCGACGT1254.8498132E-513.32000126
TAATCGA4100.013.08536620
TATACAC12300.013.08536537
AACGGAT1703.737423E-713.05882432
TATACGT850.00940966313.05882436
TCTTATA55450.012.97835937
ACTATAC2305.7661964E-1012.8695653
ATAACAC2451.1459633E-1012.8367343
TCGCTAA1451.3737261E-512.7586214
TTATACA20500.012.45365837
Produced by FastQC (version 0.11.5)