##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727604.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3373461
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13428226975204	34.0	33.0	34.0	31.0	34.0
2	33.31367548046354	34.0	33.0	34.0	31.0	34.0
3	33.40210721274086	34.0	34.0	34.0	31.0	34.0
4	36.60140550016734	37.0	37.0	37.0	35.0	37.0
5	36.58196167081819	37.0	37.0	37.0	35.0	37.0
6	36.61228898155337	37.0	37.0	37.0	35.0	37.0
7	36.618707612152626	37.0	37.0	37.0	35.0	37.0
8	36.59641863356357	37.0	37.0	37.0	35.0	37.0
9	38.263330745486606	39.0	39.0	39.0	37.0	39.0
10	38.365856015528266	39.0	39.0	39.0	37.0	39.0
11	38.469422056457745	39.0	39.0	39.0	37.0	39.0
12	38.44591266951063	39.0	39.0	39.0	37.0	39.0
13	38.482889530959454	39.0	39.0	39.0	37.0	39.0
14	40.06629304444308	41.0	40.0	41.0	38.0	41.0
15	40.08570130201594	41.0	40.0	41.0	38.0	41.0
16	40.07894088593287	41.0	40.0	41.0	38.0	41.0
17	40.07298617058267	41.0	40.0	41.0	38.0	41.0
18	40.053839365565516	41.0	40.0	41.0	38.0	41.0
19	40.0852018149906	41.0	40.0	41.0	38.0	41.0
20	40.05686326298125	41.0	40.0	41.0	38.0	41.0
21	40.01591985204512	41.0	40.0	41.0	38.0	41.0
22	39.98904448576699	41.0	40.0	41.0	38.0	41.0
23	39.947071864770336	41.0	40.0	41.0	38.0	41.0
24	39.95059465634848	41.0	40.0	41.0	38.0	41.0
25	39.9094037251357	41.0	40.0	41.0	38.0	41.0
26	39.84328616812229	41.0	40.0	41.0	38.0	41.0
27	39.812161753166855	41.0	40.0	41.0	38.0	41.0
28	39.767235785444086	41.0	40.0	41.0	38.0	41.0
29	39.71432958614313	41.0	40.0	41.0	37.0	41.0
30	39.64198904330004	41.0	40.0	41.0	37.0	41.0
31	39.58343552808229	41.0	40.0	41.0	37.0	41.0
32	39.50864912918809	41.0	40.0	41.0	36.0	41.0
33	39.40718686239444	41.0	40.0	41.0	36.0	41.0
34	39.34982085164168	41.0	39.0	41.0	36.0	41.0
35	39.29907237700392	41.0	39.0	41.0	35.0	41.0
36	39.26852303909842	41.0	39.0	41.0	35.0	41.0
37	39.164044582107216	41.0	39.0	41.0	35.0	41.0
38	39.12264051666819	41.0	39.0	41.0	35.0	41.0
39	39.04703685621384	41.0	39.0	41.0	35.0	41.0
40	38.98109241517836	41.0	39.0	41.0	35.0	41.0
41	38.88690309447775	41.0	39.0	41.0	35.0	41.0
42	38.77170863988053	41.0	38.0	41.0	35.0	41.0
43	37.588060155430874	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	3.0
13	3.0
14	1.0
15	1.0
16	6.0
17	23.0
18	54.0
19	88.0
20	149.0
21	312.0
22	565.0
23	1037.0
24	1728.0
25	2525.0
26	3779.0
27	4931.0
28	6810.0
29	9385.0
30	12549.0
31	16433.0
32	21014.0
33	28357.0
34	44131.0
35	71065.0
36	119738.0
37	237470.0
38	588510.0
39	2202791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.79619002561464	16.71944628973034	11.349679157399478	27.134684527255537
2	19.247028496846415	19.16260481446206	33.93022773940472	27.6601389492868
3	19.524221563551496	20.32716548375689	29.421594024653018	30.72701892803859
4	14.706528399172244	14.174908202584824	33.13831106984785	37.98025232839508
5	16.60745448072469	34.34203033620368	33.13718463026547	15.913330552806155
6	37.664819602183044	32.930245821724334	14.120898388924608	15.284036187168015
7	32.16622335340471	29.111704566912138	19.46185238246418	19.260219697218968
8	29.029118759635875	32.5288479694889	18.48816393608819	19.953869334787033
9	27.734661820605012	13.138465214211756	17.808387291271487	41.318485673911745
10	16.8135632811525	25.201180627254914	32.3311281796351	25.65412791195748
11	38.11370577575967	19.72469816606743	20.587106238963486	21.57448981920941
12	23.08528244435018	23.617614076463312	28.32992585359665	24.967177625589862
13	32.93074975522172	18.07490882509091	22.67762988811787	26.31671153156951
14	23.383285000182305	18.9382061923941	24.25589031561355	33.42261849181004
15	27.26487722846062	26.019627913291426	20.416154210764553	26.299340647483398
16	26.792424753094817	24.25488244861879	22.76907899631862	26.18361380196777
17	26.218533429021413	24.567261930699658	23.700318456327196	25.513886183951733
18	26.228878887291124	23.689142989944155	24.062557711501633	26.019420411263088
19	27.06780958783872	23.6682445713764	23.98925020920651	25.27469563157837
20	26.260152407275495	23.70387563395575	24.02117587842278	26.01479608034597
21	26.29065520544035	23.93186107679917	23.92771103623252	25.84977268152796
22	26.822838621818956	23.719764360696626	23.250365129461997	26.20703188802242
23	26.247346567812702	23.493112859463917	23.577625471288982	26.6819151014344
24	26.15281457233387	23.900380054786464	23.460149680105978	26.48665569277368
25	26.11166988443026	23.384500369205394	23.695634839116266	26.808194907248073
26	25.649147863277506	23.78109603164228	24.108208157734744	26.46154794734547
27	26.537760478037242	23.835817280828206	23.584710183399185	26.041712057735367
28	25.968226696558816	23.739328837653673	24.028527378855127	26.26391708693238
29	26.010972114395276	24.023636259615866	23.75071180606505	26.21467981992381
30	25.702535170852727	24.176713470231313	23.87103333935089	26.249718019565073
31	26.10494089008292	23.258694853742195	23.960259211533792	26.676105044641098
32	25.36012718095748	23.872456210402312	24.07142101242611	26.695995596214097
33	25.818736306718826	23.17456760282689	24.453046885676162	26.553649204778118
34	26.03441984359683	23.163955356235036	24.717641614946785	26.08398318522135
35	25.606491374881763	23.5957077909008	24.672969392561527	26.124831441655914
36	25.45000520237228	22.879647934272842	25.03174039954812	26.63860646380676
37	25.369909419436006	22.655842175142975	24.981495265544794	26.99275313987623
38	24.664491452546805	22.550193999574915	26.097559746503663	26.687754801374613
39	24.61303094952039	22.129943105908147	26.388270088197256	26.86875585637421
40	24.434697777742205	22.14766970775711	27.023226294894176	26.39440621960651
41	24.14813747661526	21.921966787225344	27.505431365591598	26.424464370567797
42	23.6714460312421	22.151641889442327	27.433932095257656	26.742979984057914
43	23.295630214785348	21.764769179190154	27.671255129376032	27.268345476648463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	114.0
1	135.5
2	157.0
3	515.5
4	874.0
5	874.0
6	1160.5
7	1447.0
8	1381.0
9	1315.0
10	1744.5
11	2174.0
12	2174.0
13	3369.0
14	4564.0
15	7229.0
16	9894.0
17	8880.0
18	7866.0
19	7866.0
20	9045.0
21	10224.0
22	10334.5
23	10445.0
24	13485.0
25	16525.0
26	16525.0
27	21467.0
28	26409.0
29	31974.5
30	37540.0
31	47396.5
32	57253.0
33	57253.0
34	68282.0
35	79311.0
36	91335.5
37	103360.0
38	120614.5
39	137869.0
40	137869.0
41	152362.0
42	166855.0
43	180851.0
44	194847.0
45	210290.5
46	225734.0
47	225734.0
48	244478.5
49	263223.0
50	275295.5
51	287368.0
52	289703.5
53	292039.0
54	292039.0
55	285562.5
56	279086.0
57	266385.0
58	253684.0
59	236742.5
60	219801.0
61	219801.0
62	198451.0
63	177101.0
64	156962.5
65	136824.0
66	120346.0
67	103868.0
68	103868.0
69	89447.5
70	75027.0
71	67303.0
72	59579.0
73	50747.5
74	41916.0
75	41916.0
76	36925.5
77	31935.0
78	27415.5
79	22896.0
80	19431.0
81	15966.0
82	15966.0
83	12924.0
84	9882.0
85	7881.0
86	5880.0
87	3955.0
88	2030.0
89	2030.0
90	1250.5
91	471.0
92	269.5
93	68.0
94	45.5
95	23.0
96	23.0
97	19.0
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3373461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05024686417759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.30703532515683	45.02765824847489
2	8.399402137006133	9.079795180333646
3	2.7889621743227377	4.522322820509923
4	1.3534501560541994	2.9261726021235663
5	0.8211290170915646	2.219111304056929
6	0.5628859962130098	1.8254476231021028
7	0.4025240112761039	1.5229565524762692
8	0.3167308043364565	1.3695502531100003
9	0.23777195610674984	1.1566469632453402
>10	1.587863573904567	16.324225939923334
>50	0.14503678838404546	5.422748278239223
>100	0.07450870162282053	7.305887532736128
>500	0.002313735878977788	0.8352980820991989
>1k	3.305336964885002E-4	0.30666006976845583
>5k	5.508894941475003E-5	0.15551854980101415
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5223	0.15482615628282054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.928629380923627E-5
2	0.0	0.0	0.0	0.0	5.928629380923627E-5
3	0.0	0.0	0.0	0.0	5.928629380923627E-5
4	0.0	0.0	0.0	0.0	8.892944071385441E-5
5	0.0	0.0	0.0	0.0	8.892944071385441E-5
6	0.0	0.0	0.0	0.0	1.1857258761847255E-4
7	0.0	0.0	0.0	0.0	1.1857258761847255E-4
8	0.0	0.0	0.0	0.0	1.4821573452309067E-4
9	0.0	0.0	0.0	2.9643146904618137E-5	2.371451752369451E-4
10	2.9643146904618137E-5	0.0	0.0	1.7785888142770882E-4	2.371451752369451E-4
11	2.9643146904618137E-5	0.0	0.0	2.9643146904618134E-4	2.371451752369451E-4
12	2.9643146904618137E-5	0.0	0.0	3.8536090976003576E-4	2.667883221415632E-4
13	2.9643146904618137E-5	0.0	0.0	4.44647203569272E-4	2.667883221415632E-4
14	5.928629380923627E-5	0.0	0.0	6.817923788062172E-4	4.150040566646539E-4
15	8.892944071385441E-5	2.9643146904618137E-5	0.0	0.0010671532885662529	4.742903504738902E-4
16	8.892944071385441E-5	2.9643146904618137E-5	0.0	0.0017193025204678518	5.039334973785083E-4
17	8.892944071385441E-5	2.9643146904618137E-5	0.0	0.0028160989559387227	5.039334973785083E-4
18	8.892944071385441E-5	2.9643146904618137E-5	0.0	0.003290389306412613	5.632197911877446E-4
19	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.004387185741883484	5.632197911877446E-4
20	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.006076845115446718	5.928629380923627E-4
21	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.009545093303287039	5.928629380923627E-4
22	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.014910502893022922	5.928629380923627E-4
23	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.01811196275872168	7.114355257108353E-4
24	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.024929886546783853	7.114355257108353E-4
25	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.0302360098427105	7.410786726154534E-4
26	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.03770608286267427	7.410786726154534E-4
27	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.059523438984473216	7.410786726154534E-4
28	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.15488544257662976	7.410786726154534E-4
29	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.3191084764282142	7.410786726154534E-4
30	1.1857258761847255E-4	2.9643146904618137E-5	0.0	0.5199704398539067	7.410786726154534E-4
31	1.7785888142770882E-4	2.9643146904618137E-5	0.0	1.0172638723257805	7.410786726154534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3655	0.0	16.804379	37
CTATACG	90	4.4500608E-5	16.444445	35
TATACCG	130	2.593115E-7	15.653846	5
ACCGCTA	475	0.0	14.800001	8
TCTATAC	190	4.5656634E-10	14.605264	3
CCGCTAC	535	0.0	14.177569	9
CGGTTAC	80	0.006301955	13.875	28
CGCTACG	475	0.0	13.63158	10
CCCTATA	665	0.0	13.631578	2
ATTATAC	435	0.0	13.609196	3
TCGACGT	125	4.8498132E-5	13.320001	26
TAATCGA	410	0.0	13.085366	20
TATACAC	1230	0.0	13.085365	37
AACGGAT	170	3.737423E-7	13.058824	32
TATACGT	85	0.009409663	13.058824	36
TCTTATA	5545	0.0	12.978359	37
ACTATAC	230	5.7661964E-10	12.869565	3
ATAACAC	245	1.1459633E-10	12.836734	3
TCGCTAA	145	1.3737261E-5	12.75862	14
TTATACA	2050	0.0	12.453658	37
>>END_MODULE