FastQCFastQC Report
Thu 9 Feb 2017
SRR2727603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5210294
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199120.3821665341725438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97130.18641942278113288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95980.18421225366553212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.10659667189605808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1401.873559E-917.17857210
CTTATAC67350.016.28879237
GTACCGT2251.8189894E-1214.86
GCGTAAC1702.442357E-814.14705911
GTATAAT6050.014.0661151
TATACAC27050.013.60998237
TAAGGCG4100.013.5365855
TAAGACT7050.013.1205664
GTTACGG6900.012.86956525
TCTTATA102250.012.73740937
ATTATAC4800.012.7187493
TATACCG2052.0703737E-812.6341465
GGTATCA89350.012.6094011
TCAATAC4900.012.4591843
GTTCTAA6150.012.3333331
GTATTCG3300.012.3333335
TTATACT6250.012.1359994
TACGGAT9450.011.94179927
CGTATAG1401.4001252E-411.8928571
TAGACAG8150.011.803685
Produced by FastQC (version 0.11.5)