##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5210294
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15838204139728	34.0	33.0	34.0	31.0	34.0
2	33.33015449799954	34.0	33.0	34.0	31.0	34.0
3	33.41980663663125	34.0	34.0	34.0	31.0	34.0
4	36.61025097624049	37.0	37.0	37.0	35.0	37.0
5	36.5952675607173	37.0	37.0	37.0	35.0	37.0
6	36.62885760381276	37.0	37.0	37.0	35.0	37.0
7	36.63391317265398	37.0	37.0	37.0	35.0	37.0
8	36.61343620916593	37.0	37.0	37.0	35.0	37.0
9	38.29859466663493	39.0	39.0	39.0	37.0	39.0
10	38.39407296402084	39.0	39.0	39.0	37.0	39.0
11	38.49379171309719	39.0	39.0	39.0	37.0	39.0
12	38.47114212748839	39.0	39.0	39.0	37.0	39.0
13	38.50929064655469	39.0	39.0	39.0	37.0	39.0
14	40.09195239270567	41.0	40.0	41.0	38.0	41.0
15	40.11418703052073	41.0	40.0	41.0	38.0	41.0
16	40.111860866200644	41.0	40.0	41.0	38.0	41.0
17	40.110248673107506	41.0	40.0	41.0	38.0	41.0
18	40.094591399256934	41.0	40.0	41.0	38.0	41.0
19	40.12885568453527	41.0	40.0	41.0	38.0	41.0
20	40.10397071643174	41.0	40.0	41.0	38.0	41.0
21	40.06533623630452	41.0	40.0	41.0	38.0	41.0
22	40.03887074318647	41.0	40.0	41.0	38.0	41.0
23	39.994987422974596	41.0	40.0	41.0	38.0	41.0
24	40.00130530062219	41.0	40.0	41.0	38.0	41.0
25	39.96207929917198	41.0	40.0	41.0	38.0	41.0
26	39.894456435663706	41.0	40.0	41.0	38.0	41.0
27	39.86178476684809	41.0	40.0	41.0	38.0	41.0
28	39.82468455714783	41.0	40.0	41.0	38.0	41.0
29	39.76465473925272	41.0	40.0	41.0	38.0	41.0
30	39.68905209571667	41.0	40.0	41.0	37.0	41.0
31	39.61770257110251	41.0	40.0	41.0	37.0	41.0
32	39.53892179596775	41.0	40.0	41.0	37.0	41.0
33	39.43567330365619	41.0	40.0	41.0	36.0	41.0
34	39.37530511713926	41.0	40.0	41.0	36.0	41.0
35	39.316040323252395	41.0	39.0	41.0	35.0	41.0
36	39.27532630596277	41.0	39.0	41.0	35.0	41.0
37	39.168691824300126	41.0	39.0	41.0	35.0	41.0
38	39.12469334743874	41.0	39.0	41.0	35.0	41.0
39	39.04477386496808	41.0	39.0	41.0	35.0	41.0
40	38.97787360943548	41.0	39.0	41.0	35.0	41.0
41	38.873332099877665	41.0	39.0	41.0	35.0	41.0
42	38.74693443402618	41.0	39.0	41.0	35.0	41.0
43	37.57454780862654	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	2.0
12	7.0
13	5.0
14	5.0
15	10.0
16	17.0
17	32.0
18	53.0
19	107.0
20	227.0
21	440.0
22	844.0
23	1471.0
24	2379.0
25	3715.0
26	5353.0
27	7619.0
28	10823.0
29	15222.0
30	20695.0
31	26313.0
32	32530.0
33	43483.0
34	64413.0
35	102271.0
36	170755.0
37	358467.0
38	872370.0
39	3470660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3942714173135	16.345104518094374	11.406419676125761	26.854204388466368
2	18.40773284578567	19.406601623631985	34.83647947697385	27.34918605360849
3	19.33931175476854	20.49070551489033	29.72477176911706	30.445210961224067
4	14.254243618498302	14.000994953451762	33.5003168727139	38.24444455533603
5	16.063469738943713	35.05447101449553	33.451874308820194	15.430184937740558
6	37.38760231188489	33.36790591855277	14.218468286050653	15.02602348351168
7	31.831006081422657	29.709052886459	19.51544385019348	18.94449718192486
8	29.002912311666094	33.395466743335405	18.612941995211788	18.98867894978671
9	27.794727130561153	13.159756436009177	17.698982053603885	41.34653437982578
10	16.280194553320793	25.84451088556615	32.82357962909578	25.051714932017273
11	37.91866639387336	19.80857510152018	20.991982410205644	21.280776094400817
12	22.732076155395454	23.750387214233974	29.221652367409597	24.295884262960975
13	32.630404349543426	18.194117260945355	23.209419660387688	25.966058729123535
14	23.006321716202578	18.739172875849235	25.227098509220404	33.02740689872779
15	26.76912281725369	26.263393198157342	21.234886169571237	25.732597815017733
16	26.098296948310402	24.721963866146517	23.2675353828402	25.912203802702884
17	25.598325161689534	24.755436065603973	24.540381022644787	25.105857750061706
18	25.62757495066497	24.098333030727247	24.9089590721752	25.365132946432585
19	26.434496786553694	24.061271782360073	24.94970533332668	24.554526097759553
20	25.775992679107933	23.840286171951142	24.89550493695749	25.488216211983435
21	25.589976304600086	24.372924061482905	24.921933388019944	25.115166245897065
22	26.289130709322738	23.9346954317741	24.206158040218074	25.57001581868509
23	25.486296934491605	23.93657632371609	24.560187966360438	26.016938775431864
24	25.395630265777708	23.97553765680017	24.60174032405849	26.027091753363628
25	25.55569800859606	23.535811990647744	24.61601974859768	26.292470252158516
26	25.147198987235654	23.945750470127024	25.129426477661337	25.77762406497599
27	25.93352697563708	24.220802127480713	24.49934686986953	25.346324027012678
28	25.361006499825155	24.451652824197637	24.606692059987402	25.58064861598981
29	25.34753317183253	24.599072528344852	24.623946364638925	25.429447935183696
30	25.411099642361833	24.538001118554924	24.579227199079362	25.471672040003885
31	25.273372289548345	23.819730710013676	24.73800902597819	26.168887974459793
32	24.64006829557027	24.273927728454478	25.014001129302876	26.072002846672376
33	25.148081854881895	23.715878604930932	25.22654191874777	25.909497621439403
34	25.299532041761942	23.688356165698135	25.650932557740504	25.36117923479942
35	25.02405814336005	23.803896670706106	25.59012984679943	25.581915339134415
36	24.666669481606988	23.27720854139901	26.056188000139723	25.999933976854283
37	24.5047784251714	23.07213374139732	25.93178043311951	26.49130740031177
38	23.962544148180505	22.85928586755373	26.85621962983279	26.32195035443298
39	23.79652664513749	22.513259328552284	27.479600959178118	26.210613067132105
40	23.860304236190892	22.48074676784074	27.83178837892833	25.827160617040036
41	23.338951698311075	22.37796945815342	28.362929232016466	25.920149611519044
42	22.826773306842185	22.58277555930625	28.422868268086216	26.167582865765347
43	22.433148686043435	22.155793895699553	28.556526752617028	26.85453066563998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	628.0
1	711.0
2	794.0
3	2641.0
4	4488.0
5	4488.0
6	5387.0
7	6286.0
8	5806.5
9	5327.0
10	6972.0
11	8617.0
12	8617.0
13	12972.5
14	17328.0
15	26712.5
16	36097.0
17	31566.0
18	27035.0
19	27035.0
20	28787.5
21	30540.0
22	28060.0
23	25580.0
24	30637.5
25	35695.0
26	35695.0
27	42446.5
28	49198.0
29	57188.5
30	65179.0
31	75620.5
32	86062.0
33	86062.0
34	99032.0
35	112002.0
36	129187.5
37	146373.0
38	168460.0
39	190547.0
40	190547.0
41	215043.0
42	239539.0
43	266300.0
44	293061.0
45	323434.5
46	353808.0
47	353808.0
48	387629.0
49	421450.0
50	442378.5
51	463307.0
52	469747.5
53	476188.0
54	476188.0
55	463517.5
56	450847.0
57	424969.0
58	399091.0
59	370656.5
60	342222.0
61	342222.0
62	305176.5
63	268131.0
64	232379.0
65	196627.0
66	169798.5
67	142970.0
68	142970.0
69	120207.5
70	97445.0
71	85237.0
72	73029.0
73	61149.5
74	49270.0
75	49270.0
76	42468.5
77	35667.0
78	30274.0
79	24881.0
80	20803.5
81	16726.0
82	16726.0
83	13305.0
84	9884.0
85	7876.0
86	5868.0
87	3885.0
88	1902.0
89	1902.0
90	1182.0
91	462.0
92	271.0
93	80.0
94	61.0
95	42.0
96	42.0
97	27.0
98	12.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5210294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.64133631293528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.25808246837168	44.12433466143091
2	8.833469268106608	9.476781916409697
3	3.019848616593868	4.859661457705921
4	1.4859826643304053	3.188403834101555
5	0.8925327516113993	2.3938324749748303
6	0.6084857435040885	1.9583993045357568
7	0.4341546916039806	1.6302046476917478
8	0.33780928984896097	1.449643338113756
9	0.26220287957431027	1.2658421560919082
>10	1.662129580203035	16.928266831919263
>50	0.141193852005984	5.1961236848658485
>100	0.06174141885644871	5.748821396035194
>500	0.001864733359765925	0.6332846901703538
>1k	3.586014497039125E-4	0.28507545951154956
>5k	1.0758043490778564E-4	0.47829968290138647
>10k+	3.586014496926188E-5	0.38302446354041453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19912	0.3821665341725438	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9713	0.18641942278113288	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9598	0.18421225366553212	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5554	0.10659667189605808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.9192774918267567E-5	0.0	0.0	0.0	0.0
6	1.9192774918267567E-5	0.0	0.0	1.9192774918267567E-5	3.8385549836535134E-5
7	1.9192774918267567E-5	0.0	0.0	1.9192774918267567E-5	3.8385549836535134E-5
8	1.9192774918267567E-5	0.0	0.0	3.8385549836535134E-5	3.8385549836535134E-5
9	1.9192774918267567E-5	0.0	0.0	5.7578324754802704E-5	5.7578324754802704E-5
10	1.9192774918267567E-5	0.0	0.0	3.0708439869228107E-4	5.7578324754802704E-5
11	1.9192774918267567E-5	0.0	0.0	8.060965465672378E-4	5.7578324754802704E-5
12	1.9192774918267567E-5	0.0	0.0	0.0012667231446056595	5.7578324754802704E-5
13	1.9192774918267567E-5	0.0	0.0	0.0014202653439518	9.596387459133784E-5
14	1.9192774918267567E-5	0.0	0.0	0.0017465425175623487	1.7273497426440813E-4
15	1.9192774918267567E-5	0.0	0.0	0.0024566751895382486	2.1112052410094325E-4
16	1.9192774918267567E-5	0.0	0.0	0.0034355067103698945	2.3031329901921082E-4
17	1.9192774918267567E-5	0.0	0.0	0.005354784202196651	2.3031329901921082E-4
18	1.9192774918267567E-5	0.0	0.0	0.006084109649090819	2.6869884885574594E-4
19	1.9192774918267567E-5	0.0	0.0	0.008387242639282927	2.6869884885574594E-4
20	1.9192774918267567E-5	0.0	0.0	0.011957098774080695	3.0708439869228107E-4
21	3.8385549836535134E-5	0.0	0.0	0.01783008789907057	3.2627717361054866E-4
22	3.8385549836535134E-5	0.0	0.0	0.027196162059185142	3.2627717361054866E-4
23	3.8385549836535134E-5	0.0	0.0	0.03374089830631438	3.4546994852881625E-4
24	3.8385549836535134E-5	0.0	0.0	0.04425853896152501	3.4546994852881625E-4
25	3.8385549836535134E-5	0.0	0.0	0.05151340788063015	3.4546994852881625E-4
26	3.8385549836535134E-5	0.0	0.0	0.061493650838129285	3.4546994852881625E-4
27	3.8385549836535134E-5	0.0	0.0	0.0845057879651321	3.646627234470838E-4
28	3.8385549836535134E-5	0.0	0.0	0.19734011170962715	3.838554983653514E-4
29	3.8385549836535134E-5	0.0	0.0	0.37894214798627485	3.838554983653514E-4
30	3.8385549836535134E-5	0.0	0.0	0.5961851672861455	3.838554983653514E-4
31	3.8385549836535134E-5	0.0	0.0	1.2209483764255913	3.838554983653514E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	140	1.873559E-9	17.178572	10
CTTATAC	6735	0.0	16.288792	37
GTACCGT	225	1.8189894E-12	14.8	6
GCGTAAC	170	2.442357E-8	14.147059	11
GTATAAT	605	0.0	14.066115	1
TATACAC	2705	0.0	13.609982	37
TAAGGCG	410	0.0	13.536585	5
TAAGACT	705	0.0	13.120566	4
GTTACGG	690	0.0	12.869565	25
TCTTATA	10225	0.0	12.737409	37
ATTATAC	480	0.0	12.718749	3
TATACCG	205	2.0703737E-8	12.634146	5
GGTATCA	8935	0.0	12.609401	1
TCAATAC	490	0.0	12.459184	3
GTTCTAA	615	0.0	12.333333	1
GTATTCG	330	0.0	12.333333	5
TTATACT	625	0.0	12.135999	4
TACGGAT	945	0.0	11.941799	27
CGTATAG	140	1.4001252E-4	11.892857	1
TAGACAG	815	0.0	11.80368	5
>>END_MODULE