FastQCFastQC Report
Thu 9 Feb 2017
SRR2727602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030899
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96000.31673770719512595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47390.15635624941642728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45690.15074735251818025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG200.001842411637.04
CTTATAC34350.017.18049437
GTATACG803.3848523E-416.18751
TAGACTA1151.2437613E-616.0869565
TATACCG1001.0943018E-414.85
TTATACT4900.014.7244894
GTCTAAA3000.014.1833331
GTACTAG1601.7862658E-713.8751
TACTTAC3950.013.58227831
TAGGGTG3750.013.3200015
GTCTTAG5850.013.2820521
TATACTC2102.044544E-913.2142855
TCGTTAC2408.185452E-1113.10416723
TATACAC12300.013.08536537
TAATACC2850.012.9824564
ATTATAC3150.012.9206353
CTTAGAC4300.012.9069773
TAAGACT4900.012.8367344
TCTTATA52200.012.82950137
TATTAGG3350.012.7014922
Produced by FastQC (version 0.11.5)