##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727602.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3030899
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11845429359408	34.0	33.0	34.0	31.0	34.0
2	33.29284281660326	34.0	33.0	34.0	31.0	34.0
3	33.383609945432035	34.0	34.0	34.0	31.0	34.0
4	36.596515753246806	37.0	37.0	37.0	35.0	37.0
5	36.56914994528026	37.0	37.0	37.0	35.0	37.0
6	36.60095239069332	37.0	37.0	37.0	35.0	37.0
7	36.604399882675075	37.0	37.0	37.0	35.0	37.0
8	36.581155624123404	37.0	37.0	37.0	35.0	37.0
9	38.23874401621433	39.0	39.0	39.0	37.0	39.0
10	38.32832601812201	39.0	39.0	39.0	37.0	39.0
11	38.43774206926724	39.0	39.0	39.0	37.0	39.0
12	38.41098400177637	39.0	39.0	39.0	37.0	39.0
13	38.45360897872215	39.0	39.0	39.0	37.0	39.0
14	40.02533802677028	41.0	40.0	41.0	38.0	41.0
15	40.04440035778163	41.0	40.0	41.0	38.0	41.0
16	40.04689004813423	41.0	40.0	41.0	38.0	41.0
17	40.040357332923335	41.0	40.0	41.0	38.0	41.0
18	40.02289848655465	41.0	40.0	41.0	38.0	41.0
19	40.05502393844203	41.0	40.0	41.0	38.0	41.0
20	40.030510749450904	41.0	40.0	41.0	38.0	41.0
21	39.99223629688749	41.0	40.0	41.0	38.0	41.0
22	39.96730112088856	41.0	40.0	41.0	38.0	41.0
23	39.92647725971733	41.0	40.0	41.0	38.0	41.0
24	39.93529312590093	41.0	40.0	41.0	38.0	41.0
25	39.896950376769404	41.0	40.0	41.0	38.0	41.0
26	39.83313135805581	41.0	40.0	41.0	38.0	41.0
27	39.80356983192116	41.0	40.0	41.0	38.0	41.0
28	39.77467345497161	41.0	40.0	41.0	38.0	41.0
29	39.71971154433058	41.0	40.0	41.0	38.0	41.0
30	39.6498269985242	41.0	40.0	41.0	37.0	41.0
31	39.58928588514497	41.0	40.0	41.0	37.0	41.0
32	39.51952539494058	41.0	40.0	41.0	37.0	41.0
33	39.420220205292225	41.0	40.0	41.0	36.0	41.0
34	39.37379008670365	41.0	39.0	41.0	36.0	41.0
35	39.329559315569405	41.0	39.0	41.0	36.0	41.0
36	39.299938731049764	41.0	39.0	41.0	36.0	41.0
37	39.20841440113973	41.0	39.0	41.0	35.0	41.0
38	39.17176256945547	41.0	39.0	41.0	35.0	41.0
39	39.09926592737006	41.0	39.0	41.0	35.0	41.0
40	39.044092858257564	41.0	39.0	41.0	35.0	41.0
41	38.944728610224224	41.0	39.0	41.0	35.0	41.0
42	38.83545278150146	40.0	39.0	41.0	35.0	41.0
43	37.64719840548959	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	4.0
13	3.0
14	3.0
15	4.0
16	4.0
17	13.0
18	37.0
19	75.0
20	110.0
21	235.0
22	474.0
23	895.0
24	1407.0
25	2127.0
26	3175.0
27	4504.0
28	6324.0
29	8830.0
30	11862.0
31	15190.0
32	19511.0
33	25467.0
34	37900.0
35	59166.0
36	98482.0
37	206705.0
38	564932.0
39	1963455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31292530697988	16.510150948612935	11.399818997597743	26.777104746809442
2	17.902873041958838	19.287841660180693	35.420909769675596	27.388375528184874
3	19.08842887869243	20.583727798253918	29.781691834666873	30.54615148838678
4	14.039563838979788	14.020592570059245	33.990509086577944	37.949334504383025
5	15.726456077883164	35.362016352244005	33.847152280560984	15.064375289311851
6	37.28986020319384	33.75806980041236	14.360194780492522	14.59187521590129
7	31.797034477229364	29.857642897371374	19.669015694683324	18.676306930715935
8	28.82339530284579	33.924720025312624	18.674294326534802	18.57759034530679
9	27.650344006844175	13.215847839205463	17.695343856723696	41.43846429722666
10	15.88324124294475	26.055569651116716	33.43506332609565	24.626125779842877
11	37.75800513313047	19.901323006804255	21.329942040298935	21.010729819766347
12	22.53057591163546	23.587655015888025	29.798089609716456	24.08367946276006
13	32.30077940571428	18.294308058434147	23.586203301396715	25.81870923445486
14	22.978099897093237	18.550601653172873	25.390882375163276	33.080416074570614
15	26.37135054648802	26.419422092257115	21.39810663436822	25.811120726886642
16	25.996379292084626	24.96384736013968	23.28213510248939	25.7576382452863
17	25.328887567682063	25.082920941938347	24.715241253502672	24.872950236876914
18	25.506194696689	24.423248679682168	24.916831606727904	25.153725016900925
19	26.231557039677007	24.326016802275497	24.98483123324136	24.457594924806138
20	25.65430256831389	24.097569730961013	24.90115968892398	25.346968011801117
21	25.418069028364194	24.477555999061664	25.014591380313234	25.08978359226091
22	26.080611726091828	23.976120616358383	24.477984914706823	25.465282742842966
23	25.373032885622383	24.031483728095196	24.696566926182626	25.89891646009979
24	25.354391551813503	24.047551568033114	24.681488891579694	25.916567988573686
25	25.515201925237363	23.7216416647338	24.698117621207437	26.065038788821404
26	25.156826406950543	24.086219963119852	25.084669598030157	25.672284031899444
27	25.7078180434254	24.375738023602896	24.56013875751056	25.35630517546114
28	25.45997078754521	24.52777212305656	24.55284719154284	25.45940989785539
29	25.341821024059197	24.691353951418375	24.659416232609534	25.307408791912895
30	25.347693869046772	24.672019753875006	24.62843532562451	25.35185105145371
31	25.2853691264539	23.926795317164974	24.81844495643042	25.96939059995071
32	24.71563717563667	24.398536539818714	25.032869785499283	25.852956499045334
33	25.112087205809235	23.924980674050833	25.166889427856226	25.79604269228371
34	25.336773016850778	23.786440920664134	25.56172277598165	25.31506328650344
35	25.0018558850031	23.931942304906894	25.603360587073343	25.462841223016667
36	24.85038267523926	23.570828325193286	25.74602452935581	25.832764470211643
37	24.570003817349242	23.226046133506923	25.87549106717182	26.328458981972013
38	24.12482237118426	23.223868561769955	26.550175377008607	26.10113369003718
39	24.06856843464596	22.841176825753678	27.0052878700346	26.08496686956576
40	24.125713195985746	22.911551985071096	27.33126376035625	25.631471058586907
41	23.67970691204161	22.726326413384278	27.774465595851268	25.819501078722844
42	23.202422779511952	22.86390935494716	27.880539734250466	26.05312813129042
43	22.840253007441028	22.496097692466822	27.99542314013103	26.668226159961122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	392.0
1	446.0
2	500.0
3	1531.5
4	2563.0
5	2563.0
6	2971.5
7	3380.0
8	2976.0
9	2572.0
10	3420.5
11	4269.0
12	4269.0
13	6267.0
14	8265.0
15	12688.5
16	17112.0
17	15093.5
18	13075.0
19	13075.0
20	14063.0
21	15051.0
22	14085.5
23	13120.0
24	15727.0
25	18334.0
26	18334.0
27	22079.0
28	25824.0
29	30325.0
30	34826.0
31	41101.0
32	47376.0
33	47376.0
34	55631.0
35	63886.0
36	74652.0
37	85418.0
38	99342.5
39	113267.0
40	113267.0
41	128637.5
42	144008.0
43	161005.0
44	178002.0
45	197858.0
46	217714.0
47	217714.0
48	237543.5
49	257373.0
50	270295.0
51	283217.0
52	285852.0
53	288487.0
54	288487.0
55	280538.0
56	272589.0
57	255444.5
58	238300.0
59	220266.0
60	202232.0
61	202232.0
62	179664.0
63	157096.0
64	133708.5
65	110321.0
66	93189.5
67	76058.0
68	76058.0
69	62897.0
70	49736.0
71	41123.5
72	32511.0
73	26755.0
74	20999.0
75	20999.0
76	17153.0
77	13307.0
78	11018.5
79	8730.0
80	7106.0
81	5482.0
82	5482.0
83	4300.0
84	3118.0
85	2395.5
86	1673.0
87	1115.5
88	558.0
89	558.0
90	339.0
91	120.0
92	70.0
93	20.0
94	16.0
95	12.0
96	12.0
97	8.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3030899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.50080938247593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12601000841764	47.223120468373324
2	8.93611700083516	10.276679205690503
3	3.021161622515884	5.211577157098126
4	1.5502288790037546	3.565576610832169
5	0.9245451136459271	2.6581046172627
6	0.6404150248784348	2.209462936272508
7	0.46527442481278053	1.872755920819057
8	0.35233783884082787	1.6207768727535974
9	0.2617603143675981	1.354628694631341
>10	1.6011566389821712	16.965127025369426
>50	0.0916098852320761	3.5536060036203283
>100	0.028807813789182273	2.572081089953814
>500	1.7263040358151617E-4	0.07074205797602086
>1k	3.452608070628948E-4	0.5281169256805011
>5k	5.754346784381581E-5	0.317644413666613
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9600	0.31673770719512595	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4739	0.15635624941642728	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4569	0.15074735251818025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.299351116615895E-5
2	0.0	0.0	0.0	0.0	3.299351116615895E-5
3	0.0	0.0	0.0	0.0	3.299351116615895E-5
4	0.0	3.299351116615895E-5	0.0	3.299351116615895E-5	3.299351116615895E-5
5	0.0	3.299351116615895E-5	0.0	3.299351116615895E-5	3.299351116615895E-5
6	0.0	3.299351116615895E-5	0.0	3.299351116615895E-5	3.299351116615895E-5
7	0.0	3.299351116615895E-5	0.0	3.299351116615895E-5	3.299351116615895E-5
8	0.0	3.299351116615895E-5	0.0	3.299351116615895E-5	3.299351116615895E-5
9	0.0	3.299351116615895E-5	0.0	6.59870223323179E-5	6.59870223323179E-5
10	3.299351116615895E-5	3.299351116615895E-5	0.0	2.3095457816311267E-4	9.898053349847686E-5
11	3.299351116615895E-5	3.299351116615895E-5	0.0	5.608896898247022E-4	9.898053349847686E-5
12	3.299351116615895E-5	3.299351116615895E-5	0.0	9.568118238186096E-4	1.9796106699695372E-4
13	3.299351116615895E-5	3.299351116615895E-5	0.0	0.0011217793796494044	2.639480893292716E-4
14	3.299351116615895E-5	3.299351116615895E-5	0.0	0.001385727468978676	2.639480893292716E-4
15	3.299351116615895E-5	3.299351116615895E-5	0.0	0.0017816496029725835	3.6292862282774846E-4
16	6.59870223323179E-5	3.299351116615895E-5	0.0	0.0025405003597942393	4.2891564516006636E-4
17	6.59870223323179E-5	3.299351116615895E-5	0.0	0.0035962927171113257	4.2891564516006636E-4
18	6.59870223323179E-5	3.299351116615895E-5	0.0	0.003992214851105233	4.6190915632622533E-4
19	6.59870223323179E-5	3.299351116615895E-5	0.0	0.005476922853582386	4.6190915632622533E-4
20	6.59870223323179E-5	3.299351116615895E-5	0.0	0.007720481612881195	5.278961786585432E-4
21	6.59870223323179E-5	3.299351116615895E-5	0.0	0.01161371593048795	5.938832009908611E-4
22	6.59870223323179E-5	3.299351116615895E-5	0.0	0.018344392208384377	5.938832009908611E-4
23	6.59870223323179E-5	3.299351116615895E-5	0.0	0.023359405905640538	6.598702233231791E-4
24	6.59870223323179E-5	3.299351116615895E-5	0.0	0.03154179667484796	6.598702233231791E-4
25	6.59870223323179E-5	3.299351116615895E-5	0.0	0.03741464166242425	6.598702233231791E-4
26	6.59870223323179E-5	3.299351116615895E-5	0.0	0.043815382828659086	6.598702233231791E-4
27	6.59870223323179E-5	3.299351116615895E-5	0.0	0.06275365823803433	6.598702233231791E-4
28	6.59870223323179E-5	3.299351116615895E-5	0.0	0.15701611963975046	6.598702233231791E-4
29	6.59870223323179E-5	3.299351116615895E-5	0.0	0.31360332363434085	6.598702233231791E-4
30	6.59870223323179E-5	3.299351116615895E-5	0.0	0.5045367727529027	6.598702233231791E-4
31	6.59870223323179E-5	3.299351116615895E-5	0.0	1.031014230431301	6.598702233231791E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	20	0.0018424116	37.0	4
CTTATAC	3435	0.0	17.180494	37
GTATACG	80	3.3848523E-4	16.1875	1
TAGACTA	115	1.2437613E-6	16.086956	5
TATACCG	100	1.0943018E-4	14.8	5
TTATACT	490	0.0	14.724489	4
GTCTAAA	300	0.0	14.183333	1
GTACTAG	160	1.7862658E-7	13.875	1
TACTTAC	395	0.0	13.582278	31
TAGGGTG	375	0.0	13.320001	5
GTCTTAG	585	0.0	13.282052	1
TATACTC	210	2.044544E-9	13.214285	5
TCGTTAC	240	8.185452E-11	13.104167	23
TATACAC	1230	0.0	13.085365	37
TAATACC	285	0.0	12.982456	4
ATTATAC	315	0.0	12.920635	3
CTTAGAC	430	0.0	12.906977	3
TAAGACT	490	0.0	12.836734	4
TCTTATA	5220	0.0	12.829501	37
TATTAGG	335	0.0	12.701492	2
>>END_MODULE