FastQCFastQC Report
Thu 9 Feb 2017
SRR2727600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216031
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.2741706420313298No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG28270.23247762598157445No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG18990.1561637820088468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15990.13149335831076675No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT15370.1263948040798302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14720.12104954561191286No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA13940.11463523545041203No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG13250.10896103799985363No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12360.10164214563608986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC3000.020.353
CGTATAC555.1433296E-420.1818183
TAGGCTC759.266496E-619.7333345
GTATTGG3150.019.3809531
TCGATAT609.237236E-418.537
TATACTG902.1524247E-618.55
TTGGACC4650.018.3010774
TATTGGA3650.018.2465762
GTGTTAA650.001580005517.0769231
TAATACT650.001580005517.0769234
GTCTAGC904.4462962E-516.4444451
TAATCCC3500.016.3857155
GCTATAC957.0602146E-515.57894832
GTCCATA1101.4519268E-515.1363641
TCGCATG4050.015.07407412
CTTATAC19800.014.94949537
TAACGAA750.004104587714.816
TATACAC7400.014.7537
ACCCTCG4050.014.6172848
CGTTAGG1152.2099412E-514.4782611
Produced by FastQC (version 0.11.5)