##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1216031
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13611741805924	34.0	33.0	34.0	31.0	34.0
2	33.306779185728	34.0	33.0	34.0	31.0	34.0
3	33.38865621024464	34.0	34.0	34.0	31.0	34.0
4	36.58657550670994	37.0	37.0	37.0	35.0	37.0
5	36.56965406309543	37.0	37.0	37.0	35.0	37.0
6	36.61083557902718	37.0	37.0	37.0	35.0	37.0
7	36.6104276946887	37.0	37.0	37.0	35.0	37.0
8	36.58767662995434	37.0	37.0	37.0	35.0	37.0
9	38.24278163961281	39.0	39.0	39.0	37.0	39.0
10	38.35888805466308	39.0	39.0	39.0	37.0	39.0
11	38.46874298434826	39.0	39.0	39.0	37.0	39.0
12	38.44352734428645	39.0	39.0	39.0	37.0	39.0
13	38.48413157230367	39.0	39.0	39.0	37.0	39.0
14	40.03717586147064	41.0	40.0	41.0	38.0	41.0
15	40.06001656207778	41.0	40.0	41.0	38.0	41.0
16	40.04533272589268	41.0	40.0	41.0	38.0	41.0
17	40.047446981203606	41.0	40.0	41.0	38.0	41.0
18	40.043622243182945	41.0	40.0	41.0	38.0	41.0
19	40.08193870057589	41.0	40.0	41.0	38.0	41.0
20	40.06006014649297	41.0	40.0	41.0	38.0	41.0
21	40.018184569307856	41.0	40.0	41.0	38.0	41.0
22	39.99661439552116	41.0	40.0	41.0	38.0	41.0
23	39.94837302667448	41.0	40.0	41.0	38.0	41.0
24	39.95932340540661	41.0	40.0	41.0	38.0	41.0
25	39.91616907792647	41.0	40.0	41.0	38.0	41.0
26	39.84179432925641	41.0	40.0	41.0	38.0	41.0
27	39.8188886632002	41.0	40.0	41.0	38.0	41.0
28	39.770211450201515	41.0	40.0	41.0	38.0	41.0
29	39.714660234813095	41.0	40.0	41.0	37.0	41.0
30	39.62884745536915	41.0	40.0	41.0	37.0	41.0
31	39.56420518884798	41.0	40.0	41.0	37.0	41.0
32	39.49042335269414	41.0	40.0	41.0	36.0	41.0
33	39.39044399361529	41.0	39.0	41.0	36.0	41.0
34	39.33564933788695	41.0	39.0	41.0	36.0	41.0
35	39.27787696201824	41.0	39.0	41.0	35.0	41.0
36	39.23977924904875	41.0	39.0	41.0	35.0	41.0
37	39.13796029870949	41.0	39.0	41.0	35.0	41.0
38	39.088120286407175	41.0	39.0	41.0	35.0	41.0
39	39.00798663849852	41.0	39.0	41.0	35.0	41.0
40	38.9430253011642	41.0	39.0	41.0	35.0	41.0
41	38.839479421166075	41.0	39.0	41.0	35.0	41.0
42	38.6924667216543	40.0	38.0	41.0	35.0	41.0
43	37.51017696094919	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	0.0
15	1.0
16	3.0
17	11.0
18	15.0
19	21.0
20	43.0
21	115.0
22	185.0
23	310.0
24	615.0
25	878.0
26	1294.0
27	1792.0
28	2677.0
29	3566.0
30	4833.0
31	6135.0
32	7926.0
33	10320.0
34	15567.0
35	24746.0
36	41641.0
37	87855.0
38	223717.0
39	781760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27499710122522	15.783068030338043	12.145825229784437	26.796109638652304
2	18.97089794585829	19.047458494067996	34.185148240464265	27.79649531960945
3	20.31757414079082	19.94159688363208	29.596613902112693	30.14421507346441
4	15.310218242791507	13.480577386596229	31.371815356680877	39.83738901393139
5	17.107705313433623	33.58524577087262	32.71660015246322	16.59044876323054
6	38.83996378381801	31.03193915286699	13.300976702074207	16.82712036124079
7	31.760785703653937	28.840300946275217	18.613259036981788	20.785654313089058
8	29.283546225384054	32.35616526223427	18.371159945757963	19.989128566623712
9	27.110575306057168	13.530658346703333	17.273737264921703	42.0850290823178
10	17.419374999486035	25.05700923742898	31.69746494949553	25.826150813589454
11	37.913260434972464	19.051899170333648	19.42491597664862	23.609924418045264
12	23.311658995535474	23.533363869835554	27.57881994784672	25.576157186782243
13	34.77271549820687	17.091422833792887	21.747389663585878	26.38847200441436
14	23.471852280081677	18.74401228258161	24.481612722044094	33.30252271529262
15	28.574929422029538	25.206840944021984	20.214862943461146	26.003366690487333
16	26.85762122840618	23.416014887778356	22.850157602890057	26.876206280925402
17	26.47210474075085	23.68656720100063	23.66831108746405	26.173016970784463
18	26.792162370860613	23.04826110518564	23.964356171841015	26.195220352112734
19	27.374795543863602	23.3318887429679	24.103497361498185	25.18981835167031
20	27.24642710588793	22.550000781230082	23.37571986240482	26.827852250477168
21	26.447845490781074	24.04280811920091	23.627522653616563	25.881823736401454
22	27.003505667207495	23.71000410351381	22.406172211070277	26.880318018208417
23	26.20607533853989	22.993246060338922	23.19620141262846	27.604477188492726
24	26.348341448532153	23.166514669445103	23.303764459952088	27.181379422070655
25	26.82069782760472	22.21933486893015	22.85402263593609	28.105944667529037
26	25.885442065210505	23.512065070709546	24.185649872412792	26.416842991667156
27	27.406291451451487	22.888232290130762	23.538544658812153	26.166931599605604
28	26.192835544488585	24.062873397142013	23.253765734590647	26.49052532377875
29	26.60639408041407	23.39627854881989	23.505321821565403	26.492005549200638
30	26.186092295344444	23.268074580335536	24.25875656130477	26.287076563015255
31	26.441431180619574	22.89127497572019	24.008269526023597	26.659024317636636
32	25.505188601277435	22.707891492897797	23.776860951735607	28.01005895408916
33	26.00270881252205	22.63215329214469	24.115997042838547	27.249140852494712
34	26.47210474075085	23.007061497609847	24.19642262409429	26.324411137545013
35	25.745642997588053	23.34611535396713	24.195600276637684	26.712641371807134
36	25.700496122220567	22.683303303945376	25.005448051899993	26.61075252193406
37	24.92206201980048	22.461351725408317	24.971567336687965	27.645018918103236
38	24.907342000327297	21.870001669365337	25.955259364276074	27.267396966031292
39	24.232359207947823	21.5693514392314	26.842325565713377	27.355963787107402
40	24.69024227178419	21.632178784915844	27.10342088318472	26.574158060115245
41	24.60364908460393	21.183423777847768	27.517555062329823	26.695372075218476
42	23.3559012887007	21.89245175493059	27.664179613842084	27.08746734252663
43	23.26988374474006	21.640155555244892	27.572323402939563	27.51763729707549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	35.5
2	44.0
3	263.0
4	482.0
5	482.0
6	642.5
7	803.0
8	809.5
9	816.0
10	1111.0
11	1406.0
12	1406.0
13	2150.0
14	2894.0
15	4525.5
16	6157.0
17	5158.5
18	4160.0
19	4160.0
20	4155.0
21	4150.0
22	3280.0
23	2410.0
24	2932.0
25	3454.0
26	3454.0
27	4280.0
28	5106.0
29	6313.5
30	7521.0
31	9094.5
32	10668.0
33	10668.0
34	12898.5
35	15129.0
36	18598.0
37	22067.0
38	27131.0
39	32195.0
40	32195.0
41	38089.5
42	43984.0
43	52055.5
44	60127.0
45	70204.5
46	80282.0
47	80282.0
48	96924.5
49	113567.0
50	119750.5
51	125934.0
52	130194.5
53	134455.0
54	134455.0
55	130376.5
56	126298.0
57	111628.5
58	96959.0
59	89759.5
60	82560.0
61	82560.0
62	74330.5
63	66101.0
64	57269.5
65	48438.0
66	42449.5
67	36461.0
68	36461.0
69	31055.5
70	25650.0
71	22554.5
72	19459.0
73	16418.5
74	13378.0
75	13378.0
76	11484.5
77	9591.0
78	7837.0
79	6083.0
80	4864.5
81	3646.0
82	3646.0
83	2838.0
84	2030.0
85	1558.5
86	1087.0
87	714.0
88	341.0
89	341.0
90	215.5
91	90.0
92	51.0
93	12.0
94	10.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1216031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.25882226433629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3500809569058	56.551958443703356
2	8.044676994019573	10.660616462414762
3	2.5764091900964856	5.12129515820417
4	1.2545823856493943	3.325086052268409
5	0.7081442088356639	2.346040063538065
6	0.4520451337403705	1.7971186903176803
7	0.30904262147944644	1.4333760090097838
8	0.23181540486779034	1.2287852567416064
9	0.16653130832801386	0.993075152395793
>10	0.8455572538758832	9.615684747871006
>50	0.03520311373790172	1.5554304971250308
>100	0.022298789795767916	2.8163912452790583
>500	0.0022423274490108967	1.0088172963776736
>1k	0.0013703112188399925	1.5463249247536688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3334	0.2741706420313298	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2827	0.23247762598157445	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	1899	0.1561637820088468	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.13149335831076675	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	1537	0.1263948040798302	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1472	0.12104954561191286	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA	1394	0.11463523545041203	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	1325	0.10896103799985363	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1236	0.10164214563608986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.223474566026689E-5
2	0.0	0.0	0.0	0.0	8.223474566026689E-5
3	0.0	0.0	0.0	0.0	8.223474566026689E-5
4	0.0	0.0	0.0	0.0	8.223474566026689E-5
5	1.6446949132053378E-4	0.0	0.0	0.0	8.223474566026689E-5
6	2.4670423698080064E-4	0.0	0.0	0.0	1.6446949132053378E-4
7	2.4670423698080064E-4	0.0	0.0	0.0	1.6446949132053378E-4
8	2.4670423698080064E-4	0.0	0.0	0.0	2.4670423698080064E-4
9	2.4670423698080064E-4	0.0	0.0	0.0	2.4670423698080064E-4
10	2.4670423698080064E-4	0.0	0.0	0.0	2.4670423698080064E-4
11	2.4670423698080064E-4	0.0	0.0	4.111737283013344E-4	2.4670423698080064E-4
12	2.4670423698080064E-4	0.0	0.0	5.756432196218681E-4	2.4670423698080064E-4
13	2.4670423698080064E-4	0.0	0.0	5.756432196218681E-4	2.4670423698080064E-4
14	2.4670423698080064E-4	0.0	0.0	0.0012335211849040034	3.2893898264106756E-4
15	2.4670423698080064E-4	0.0	0.0	0.0018913991501861383	3.2893898264106756E-4
16	2.4670423698080064E-4	0.0	0.0	0.0033716245720709424	3.2893898264106756E-4
17	2.4670423698080064E-4	0.0	0.0	0.006249840670180283	3.2893898264106756E-4
18	2.4670423698080064E-4	0.0	0.0	0.008223474566026689	4.111737283013344E-4
19	3.2893898264106756E-4	0.0	0.0	0.013568733033944036	4.111737283013344E-4
20	3.2893898264106756E-4	0.0	0.0	0.018831756756201117	4.111737283013344E-4
21	3.2893898264106756E-4	0.0	0.0	0.03067356013127955	4.111737283013344E-4
22	4.111737283013344E-4	0.0	0.0	0.04564028384144812	4.111737283013344E-4
23	4.934084739616013E-4	0.0	0.0	0.056413035522943085	4.934084739616013E-4
24	4.934084739616013E-4	0.0	0.0	0.08239921515158742	4.934084739616013E-4
25	4.934084739616013E-4	0.0	0.0	0.10114873716212827	5.756432196218681E-4
26	4.934084739616013E-4	0.0	0.0	0.1316578278020873	6.578779652821351E-4
27	4.934084739616013E-4	0.0	0.0	0.17014368877109218	6.578779652821351E-4
28	4.934084739616013E-4	0.0	0.0	0.3211266818033422	6.578779652821351E-4
29	4.934084739616013E-4	0.0	0.0	0.6018761034874933	6.578779652821351E-4
30	5.756432196218681E-4	0.0	0.0	0.9331176590070484	6.578779652821351E-4
31	6.578779652821351E-4	0.0	0.0	1.571094815839399	6.578779652821351E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGAC	300	0.0	20.35	3
CGTATAC	55	5.1433296E-4	20.181818	3
TAGGCTC	75	9.266496E-6	19.733334	5
GTATTGG	315	0.0	19.380953	1
TCGATAT	60	9.237236E-4	18.5	37
TATACTG	90	2.1524247E-6	18.5	5
TTGGACC	465	0.0	18.301077	4
TATTGGA	365	0.0	18.246576	2
GTGTTAA	65	0.0015800055	17.076923	1
TAATACT	65	0.0015800055	17.076923	4
GTCTAGC	90	4.4462962E-5	16.444445	1
TAATCCC	350	0.0	16.385715	5
GCTATAC	95	7.0602146E-5	15.578948	32
GTCCATA	110	1.4519268E-5	15.136364	1
TCGCATG	405	0.0	15.074074	12
CTTATAC	1980	0.0	14.949495	37
TAACGAA	75	0.0041045877	14.8	16
TATACAC	740	0.0	14.75	37
ACCCTCG	405	0.0	14.617284	8
CGTTAGG	115	2.2099412E-5	14.478261	1
>>END_MODULE