##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3209213
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.658030800697865	31.0	31.0	34.0	30.0	34.0
2	31.902601665891297	31.0	31.0	34.0	30.0	34.0
3	32.01659285313876	33.0	31.0	34.0	30.0	34.0
4	35.73578600111616	37.0	35.0	37.0	35.0	37.0
5	35.34841563959762	37.0	35.0	37.0	33.0	37.0
6	35.3654017355657	37.0	35.0	37.0	33.0	37.0
7	35.31796144412976	37.0	35.0	37.0	33.0	37.0
8	35.27480506903094	37.0	35.0	37.0	32.0	37.0
9	37.11583462986096	39.0	37.0	39.0	33.0	39.0
10	36.88192151783007	39.0	37.0	39.0	33.0	39.0
11	37.001250462340764	39.0	37.0	39.0	33.0	39.0
12	36.885201138098346	39.0	37.0	39.0	33.0	39.0
13	37.01657883100935	39.0	37.0	39.0	33.0	39.0
14	38.0614633556576	40.0	37.0	41.0	33.0	41.0
15	38.068592517854064	40.0	37.0	41.0	33.0	41.0
16	38.015697306473584	40.0	37.0	41.0	33.0	41.0
17	37.93138785116476	40.0	37.0	41.0	33.0	41.0
18	37.95518402798443	40.0	37.0	41.0	33.0	41.0
19	38.003640144795625	40.0	37.0	41.0	33.0	41.0
20	37.88605586478679	40.0	37.0	41.0	33.0	41.0
21	37.880112974738665	39.0	37.0	41.0	33.0	41.0
22	37.782461619094775	39.0	37.0	41.0	33.0	41.0
23	37.721255959015494	39.0	37.0	41.0	32.0	41.0
24	37.62471983006426	39.0	37.0	41.0	32.0	41.0
25	37.59817531587962	39.0	37.0	41.0	32.0	41.0
26	37.46367785497566	39.0	36.0	40.0	32.0	41.0
27	37.274130137201865	39.0	36.0	40.0	32.0	41.0
28	37.16690353678612	39.0	36.0	40.0	31.0	41.0
29	37.08487470292561	39.0	36.0	40.0	31.0	41.0
30	37.02830413562453	39.0	36.0	40.0	31.0	41.0
31	36.973862127568346	39.0	36.0	40.0	31.0	41.0
32	36.867623619871914	39.0	36.0	40.0	31.0	41.0
33	36.831042377056306	39.0	35.0	40.0	31.0	41.0
34	36.81996209039413	39.0	35.0	40.0	31.0	41.0
35	36.706359471932835	39.0	35.0	40.0	30.0	41.0
36	36.6182574980221	39.0	35.0	40.0	30.0	41.0
37	36.48892111555076	39.0	35.0	40.0	30.0	41.0
38	36.421115083355325	38.0	35.0	40.0	30.0	41.0
39	36.30620778365288	38.0	35.0	40.0	30.0	41.0
40	36.24720359789145	38.0	35.0	40.0	30.0	41.0
41	36.18374193299105	38.0	35.0	40.0	30.0	41.0
42	36.10460695503851	38.0	35.0	40.0	29.0	41.0
43	34.793985316649284	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	10.0
12	4.0
13	3.0
14	3.0
15	5.0
16	11.0
17	17.0
18	85.0
19	162.0
20	389.0
21	816.0
22	1686.0
23	3094.0
24	5161.0
25	8751.0
26	13907.0
27	21359.0
28	31684.0
29	45355.0
30	62588.0
31	83863.0
32	111250.0
33	147108.0
34	196012.0
35	264339.0
36	366146.0
37	526212.0
38	790219.0
39	528964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.530396081531514	18.550435885682877	12.94064931184063	25.978518720944983
2	18.056888090631563	20.921297526839137	36.23449113536559	24.787323247163712
3	20.03453806275869	22.888882726076456	30.03842375062048	27.038155460544377
4	14.870624043963426	15.940605999040885	34.163827704798656	35.024942252197036
5	15.342795881731753	35.954702913144125	33.73484402562248	14.967657179501643
6	34.93454625791432	34.13257393635137	15.548297978351702	15.3845818273826
7	28.79338329989315	30.50121634182586	21.644060397362217	19.061339960918765
8	26.144384931757415	35.11181713398269	20.00982795470416	18.733969979555734
9	26.744033506034032	14.976569021750816	19.901358993622424	38.37803847859272
10	17.120490288428968	26.666257428223055	33.20060089498578	23.012651388362194
11	35.02918005130853	21.88963462381587	21.80852439523335	21.27266092964225
12	21.361654710983657	24.92442851253563	30.765891824568826	22.948024951911886
13	30.71912023290445	19.89877268975291	24.730798485485384	24.65130859185726
14	22.046370870366037	20.719503504441743	26.556791337938616	30.677334287253604
15	25.068825285202323	27.40497436598942	23.33064212316228	24.19555822564598
16	23.847155050163387	26.013480563614817	25.71216058267245	24.427203803549343
17	23.613328252129104	26.070846653057934	26.30760251812516	24.008222576687803
18	23.624234352783688	25.4164494534953	27.00590456289439	23.953411630826622
19	24.780623785333038	25.085402558197288	27.45642000079147	22.6775536556782
20	24.171720605643817	25.00519597795472	27.120792543218542	23.70229087318293
21	23.820824607154464	25.460478939852234	27.602343627549807	23.116352825443496
22	24.167046562506133	25.08290973519053	26.811090444915937	23.938953257387404
23	23.66396371945396	25.365627024444937	27.04142729074075	23.928981965360354
24	23.574440213223617	25.100858060839215	27.203429625892706	24.121272100044465
25	23.887788065173613	25.016164399184472	26.958759047779	24.13728848786291
26	23.51243124093041	25.447485099929484	27.232034769895296	23.80804888924481
27	23.882771258872502	25.8445606446191	26.754721484675525	23.517946611832873
28	23.567460308804684	26.02008654458274	26.47783116919943	23.934621977413155
29	23.587496373721535	26.088483375830773	27.116180820656027	23.207839429791665
30	24.18290714888666	25.615376729434914	26.572215680292956	23.629500441385474
31	23.624608276234703	25.31913587536882	27.212684231305307	23.84357161709117
32	23.12647991890847	25.772923143462272	27.035849599263123	24.064747338366136
33	23.225444992276923	25.20521386395979	27.212684231305307	24.35665691245798
34	23.656859173884687	25.22606009635384	27.520579032928012	23.59650169683346
35	23.462169697056567	25.161745262779377	27.370760370221607	24.005324669942443
36	23.35423046086377	24.927856144169926	27.64275852054694	24.075154874419365
37	22.94793147104913	24.621581677501617	27.9813462054404	24.44914064600885
38	22.937305812982807	24.465998361592078	28.248701472915634	24.347994352509478
39	22.358908554838834	24.616190947749494	28.91157427070126	24.11332622671041
40	22.91966909020997	24.323190763592194	28.88150459318219	23.875635553015645
41	22.243335048187827	24.573999918360045	28.97464269277234	24.208022340679786
42	21.90711554515079	24.93524113232746	28.805535812051115	24.352107510470635
43	21.60557744219533	24.54221642502383	28.775341493381713	25.076864639399133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2250.0
1	2123.5
2	1997.0
3	3788.0
4	5579.0
5	5579.0
6	6536.5
7	7494.0
8	6819.0
9	6144.0
10	8203.5
11	10263.0
12	10263.0
13	16229.5
14	22196.0
15	27010.5
16	31825.0
17	29481.0
18	27137.0
19	27137.0
20	30248.0
21	33359.0
22	29342.5
23	25326.0
24	29200.5
25	33075.0
26	33075.0
27	38823.5
28	44572.0
29	50578.5
30	56585.0
31	64559.0
32	72533.0
33	72533.0
34	82817.0
35	93101.0
36	105177.0
37	117253.0
38	130874.5
39	144496.0
40	144496.0
41	160555.5
42	176615.0
43	192832.5
44	209050.0
45	227286.0
46	245522.0
47	245522.0
48	260639.0
49	275756.0
50	282985.5
51	290215.0
52	284447.0
53	278679.0
54	278679.0
55	264282.5
56	249886.0
57	230411.0
58	210936.0
59	192598.5
60	174261.0
61	174261.0
62	151871.0
63	129481.0
64	108744.0
65	88007.0
66	72749.5
67	57492.0
68	57492.0
69	47000.0
70	36508.0
71	29294.5
72	22081.0
73	17446.5
74	12812.0
75	12812.0
76	10240.0
77	7668.0
78	6079.5
79	4491.0
80	3481.0
81	2471.0
82	2471.0
83	1830.5
84	1190.0
85	899.5
86	609.0
87	408.0
88	207.0
89	207.0
90	139.5
91	72.0
92	43.0
93	14.0
94	9.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3209213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.15862598240076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91331064495445	53.837627126146415
2	8.662686092073413	11.11572073968566
3	2.8402251275799264	5.46674824998651
4	1.382766972529533	3.5486571604535575
5	0.7994824872781131	2.5646848940377964
6	0.4982628178241943	1.9180714661831744
7	0.35537932194749267	1.5960454299095859
8	0.25889000301162757	1.3288021499044496
9	0.20592474812561878	1.1890664005960494
>10	1.0180272164389443	11.450021576403032
>50	0.04696205855794924	2.0355963513905966
>100	0.017014208872741222	1.9404663627813845
>500	5.827095315202128E-4	0.24082713777632217
>1k	2.913547646403698E-4	0.41573057133000196
>5k	4.8559127440061624E-5	0.23048372052603075
>10k+	1.456773823201849E-4	1.1214506628892948
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12975	0.4043047314092271	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12960	0.4038373270954593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10061	0.31350365338791786	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7398	0.23052380755032462	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.232057516905235E-5
2	0.0	0.0	0.0	0.0	6.232057516905235E-5
3	0.0	0.0	0.0	3.116028758452617E-5	6.232057516905235E-5
4	0.0	0.0	0.0	6.232057516905235E-5	6.232057516905235E-5
5	0.0	0.0	0.0	9.348086275357852E-5	6.232057516905235E-5
6	0.0	0.0	0.0	1.246411503381047E-4	9.348086275357852E-5
7	0.0	0.0	0.0	1.246411503381047E-4	9.348086275357852E-5
8	0.0	0.0	0.0	1.246411503381047E-4	9.348086275357852E-5
9	0.0	0.0	0.0	1.246411503381047E-4	1.8696172550715704E-4
10	0.0	0.0	0.0	1.246411503381047E-4	1.8696172550715704E-4
11	0.0	0.0	0.0	1.5580143792263086E-4	1.8696172550715704E-4
12	0.0	0.0	0.0	2.1812201309168324E-4	2.1812201309168324E-4
13	0.0	0.0	0.0	2.492823006762094E-4	3.1160287584526173E-4
14	0.0	0.0	0.0	3.739234510143141E-4	3.4276316342978793E-4
15	0.0	0.0	0.0	4.674043137678926E-4	3.739234510143141E-4
16	0.0	0.0	0.0	5.920454641059974E-4	4.362440261833665E-4
17	0.0	0.0	0.0	9.348086275357852E-4	4.362440261833665E-4
18	0.0	0.0	0.0	0.00105944977787389	4.985646013524188E-4
19	0.0	0.0	0.0	0.0013398923661346255	4.985646013524188E-4
20	0.0	0.0	0.0	0.001807296679902518	5.608851765214712E-4
21	3.116028758452617E-5	0.0	0.0	0.0028979067453609344	5.920454641059974E-4
22	3.116028758452617E-5	0.0	0.0	0.004861004863186084	6.232057516905235E-4
23	3.116028758452617E-5	0.0	0.0	0.006450179529996918	7.166866144441021E-4
24	3.116028758452617E-5	3.116028758452617E-5	0.0	0.009722009726372167	7.166866144441021E-4
25	3.116028758452617E-5	3.116028758452617E-5	0.0	0.012495275321394996	7.166866144441021E-4
26	3.116028758452617E-5	3.116028758452617E-5	0.0	0.01701351702115129	7.166866144441021E-4
27	3.116028758452617E-5	3.116028758452617E-5	0.0	0.0410692590364055	7.478469020286282E-4
28	3.116028758452617E-5	3.116028758452617E-5	0.0	0.10616309980048068	7.478469020286282E-4
29	3.116028758452617E-5	3.116028758452617E-5	0.0	0.2018875032601451	7.478469020286282E-4
30	3.116028758452617E-5	3.116028758452617E-5	0.0	0.3241916320294103	7.478469020286282E-4
31	3.116028758452617E-5	3.116028758452617E-5	0.0	0.7101741143389361	7.478469020286282E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2820	0.0	17.319149	37
GGTATCA	5970	0.0	16.268847	1
TCGTTAC	580	0.0	14.353448	23
TATACCG	120	3.3039843E-5	13.874999	5
TCTTATA	4215	0.0	13.386713	37
CGTTACG	525	0.0	13.038095	24
CTCTTAT	6580	0.0	12.792552	37
TAATACC	350	0.0	12.685715	4
TACGGAT	1090	0.0	12.220183	27
CTCGTTA	700	0.0	12.157144	22
GGACCGT	215	4.0196028E-8	12.046513	6
GTATCAA	8130	0.0	11.946494	2
ATACCCG	250	2.1500455E-9	11.840001	5
ACGGATG	1145	0.0	11.633187	28
GTATTAG	305	3.092282E-11	11.52459	1
GTAATAC	290	1.5643309E-10	11.4827585	3
GTTACGG	920	0.0	11.461957	25
GGGTAAG	1840	0.0	11.361413	1
TATACGG	115	0.0052219247	11.26087	2
TACTTAC	1085	0.0	11.253456	31
>>END_MODULE