FastQCFastQC Report
Thu 9 Feb 2017
SRR2727598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420484
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66010.4647007639649584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57440.40436921499995776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44190.3110911492139299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.20274779582170585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.019.8631571
CTTATAC14200.017.19718437
GGTTAGT1151.2426226E-616.0869561
CGTAGAC700.002592897315.8571433
GTACTAG1001.09365195E-414.81
GTATTAC1252.9597504E-614.7999991
GTATCAA32900.014.3951362
TCTTATA22350.013.9060437
GTCCTAC2051.4242687E-913.5365851
GTACTAA1153.5780916E-412.8695651
TACGGAT3950.012.6455727
GAGTGTC4400.012.6136367
TATACTG2204.08545E-912.6136365
GGAGTGT5350.012.4485996
GCACCCG6050.012.2314059
CTAGAGT2154.0126906E-812.0465124
TGTATAC1705.3104304E-611.9705893
GTATAAT1401.398749E-411.8928581
GTAAGGT1401.398749E-411.8928584
GTAAGAG6400.011.85156253
Produced by FastQC (version 0.11.5)