##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1420484
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716468471309778	31.0	31.0	34.0	30.0	34.0
2	31.964180518752762	31.0	31.0	34.0	30.0	34.0
3	32.07744191416447	33.0	31.0	34.0	30.0	34.0
4	35.78744075962841	37.0	35.0	37.0	35.0	37.0
5	35.40134348574148	37.0	35.0	37.0	33.0	37.0
6	35.41293742132963	37.0	35.0	37.0	33.0	37.0
7	35.37790499576201	37.0	35.0	37.0	33.0	37.0
8	35.328881564311885	37.0	35.0	37.0	33.0	37.0
9	37.17092343173172	39.0	37.0	39.0	34.0	39.0
10	36.948516843554735	39.0	37.0	39.0	33.0	39.0
11	37.054593363952	39.0	37.0	39.0	33.0	39.0
12	36.9494848234827	39.0	37.0	39.0	33.0	39.0
13	37.07305890105063	39.0	37.0	39.0	33.0	39.0
14	38.13528909864525	40.0	37.0	41.0	33.0	41.0
15	38.13429084734499	40.0	37.0	41.0	33.0	41.0
16	38.08561025678571	40.0	37.0	41.0	33.0	41.0
17	38.008340115059376	40.0	37.0	41.0	33.0	41.0
18	38.0270344474137	40.0	37.0	41.0	33.0	41.0
19	38.07723423847083	40.0	37.0	41.0	33.0	41.0
20	37.956660546686905	40.0	37.0	41.0	33.0	41.0
21	37.94629084171311	40.0	37.0	41.0	33.0	41.0
22	37.85342249543113	39.0	37.0	41.0	33.0	41.0
23	37.78624187248853	39.0	37.0	41.0	33.0	41.0
24	37.69075118058352	39.0	37.0	41.0	32.0	41.0
25	37.670036410125	39.0	37.0	41.0	32.0	41.0
26	37.5362644000214	39.0	37.0	41.0	32.0	41.0
27	37.356070184528654	39.0	36.0	40.0	32.0	41.0
28	37.26238873510719	39.0	36.0	40.0	32.0	41.0
29	37.18137972690998	39.0	36.0	40.0	31.0	41.0
30	37.12784797294443	39.0	36.0	40.0	31.0	41.0
31	37.07881538968408	39.0	36.0	40.0	31.0	41.0
32	36.96938437884552	39.0	36.0	40.0	31.0	41.0
33	36.93293131073634	39.0	36.0	40.0	31.0	41.0
34	36.9231670332084	39.0	36.0	40.0	31.0	41.0
35	36.81958895700339	39.0	35.0	40.0	31.0	41.0
36	36.74140926613746	39.0	35.0	40.0	30.0	41.0
37	36.6106841048544	39.0	35.0	40.0	30.0	41.0
38	36.55660676220218	39.0	35.0	40.0	30.0	41.0
39	36.44955381405211	39.0	35.0	40.0	30.0	41.0
40	36.383728363008665	39.0	35.0	40.0	30.0	41.0
41	36.332495825366564	39.0	35.0	40.0	30.0	41.0
42	36.261167320434446	39.0	35.0	40.0	30.0	41.0
43	34.96744349109177	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	2.0
17	9.0
18	24.0
19	60.0
20	149.0
21	372.0
22	643.0
23	1296.0
24	2171.0
25	3625.0
26	5725.0
27	8886.0
28	13405.0
29	19014.0
30	26136.0
31	35301.0
32	47135.0
33	62541.0
34	83810.0
35	114386.0
36	160359.0
37	233013.0
38	355437.0
39	246982.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.789559051703506	19.31911939874015	13.458863317010259	25.43245823254609
2	17.824910382658306	21.27429805615551	36.84117526138978	24.059616299796406
3	19.705959377226353	23.444614652470566	30.503335482835425	26.34609048746765
4	14.72519225841333	16.581390568285176	34.72386876585727	33.96954840744422
5	14.927658460074172	36.13148757747359	34.22206797119855	14.718785991253686
6	33.71005938820852	34.895570805443775	16.033901121026354	15.36046868532134
7	27.907811703616513	30.751560735636584	22.31781561777535	19.02281194297155
8	25.286310863057942	35.66157732153266	20.38685405819425	18.665257757215144
9	26.11532407264003	15.477048667918822	20.647962243854913	37.759665015586236
10	17.086711289954692	26.920542575629153	33.6505022231859	22.342243911230256
11	34.21629529090085	22.57160235525356	22.288107433804253	20.923994920041338
12	20.826563340382574	25.4329510223276	31.160998645532086	22.57948699175774
13	29.933529698328176	20.581576420431347	25.168745300897438	24.31614858034304
14	21.605311992250527	21.478876214022826	26.984816442846242	29.9309953508804
15	24.669549252226705	28.040160959222348	23.68066095781438	23.609628830736565
16	23.273968590987298	26.615435302333573	26.272383215861634	23.838212890817495
17	22.9674533468874	26.947364419451397	26.734197639677742	23.35098459398346
18	22.976957149816542	26.206771776380442	27.49710661999713	23.319164453805886
19	24.133675564103502	25.71524916859324	28.050157551933	22.100917715370255
20	23.59421155042929	25.71236282844439	27.639030077072324	23.054395544054
21	23.3205724246102	26.111170558767295	28.014465492043556	22.553791524578944
22	23.622300567975422	25.635839615229738	27.433184745481114	23.308675071313722
23	23.121978142661234	26.05295096600877	27.409671633049015	23.41539925828098
24	23.025391345485062	25.9146882330248	27.63586214276261	23.424058278727532
25	23.332892169147982	25.649356134951184	27.48239332509201	23.535358370808822
26	22.87262651321662	25.95544898780979	27.8385395400441	23.333384958929493
27	23.26573196178204	26.457883369330453	27.34912888846337	22.92725578042414
28	23.01792910022218	26.41261710797165	27.151308990456773	23.4181448013494
29	22.95752715271696	26.552851000081663	27.79439965532875	22.69522219187263
30	23.48122189338282	26.07062099960295	27.26831136429555	23.17984574271868
31	23.12028857769605	25.732708006566774	27.826994179448693	23.320009236288477
32	22.692265453183563	26.29512194435136	27.54004972952881	23.47256287293627
33	22.755412943757197	25.74453496132304	27.63924127269297	23.860810822226792
34	23.123808504706847	25.72123304451159	28.011015963572977	23.143942487208584
35	22.847705429980202	25.642879469251323	27.865642978027203	23.643772122741264
36	22.675017810830674	25.248647644042453	28.377510763936797	23.69882378119007
37	22.406306582826698	24.85427502175315	28.66417361969582	24.07524477572433
38	22.26769185714165	24.717631455194145	29.131127136947686	23.883549550716516
39	21.719146431779592	24.89552856631965	29.669183179817583	23.716141822083177
40	22.156954953382087	24.584859808347016	29.64792282067239	23.61026241759851
41	21.56335446228187	24.892853421791447	29.733457047034673	23.810335068892012
42	21.289574539382354	25.266951264498577	29.43109531680751	24.01237887931156
43	20.97088034782511	25.033087313901458	29.39392488757353	24.602107450699904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1505.0
1	1355.5
2	1206.0
3	1986.0
4	2766.0
5	2766.0
6	3180.0
7	3594.0
8	3266.5
9	2939.0
10	3811.0
11	4683.0
12	4683.0
13	7274.5
14	9866.0
15	12103.0
16	14340.0
17	13470.0
18	12600.0
19	12600.0
20	14696.0
21	16792.0
22	14809.5
23	12827.0
24	14971.0
25	17115.0
26	17115.0
27	20524.5
28	23934.0
29	26837.0
30	29740.0
31	34278.5
32	38817.0
33	38817.0
34	43716.0
35	48615.0
36	54392.5
37	60170.0
38	66210.0
39	72250.0
40	72250.0
41	78767.5
42	85285.0
43	92207.0
44	99129.0
45	105071.0
46	111013.0
47	111013.0
48	115220.5
49	119428.0
50	120352.5
51	121277.0
52	117462.5
53	113648.0
54	113648.0
55	107090.5
56	100533.0
57	92370.0
58	84207.0
59	76711.0
60	69215.0
61	69215.0
62	60171.5
63	51128.0
64	42773.5
65	34419.0
66	28678.0
67	22937.0
68	22937.0
69	18534.0
70	14131.0
71	11456.5
72	8782.0
73	6950.0
74	5118.0
75	5118.0
76	4096.0
77	3074.0
78	2403.5
79	1733.0
80	1330.5
81	928.0
82	928.0
83	688.0
84	448.0
85	323.5
86	199.0
87	130.5
88	62.0
89	62.0
90	41.5
91	21.0
92	14.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1420484.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.00122314537256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40985431395649	63.81026279877643
2	7.463603941093651	10.897044335449193
3	2.1238383850552864	4.651283996163859
4	0.9660447164666953	2.8208978366077355
5	0.5396411337018572	1.969723140989555
6	0.3623218649191725	1.5869963586847229
7	0.2332704117585709	1.1920317767400261
8	0.17302811851047942	1.010501143184598
9	0.11634957402994514	0.7644295094966163
>10	0.5806281334975233	7.517718193458274
>50	0.02331363360845361	1.1347409077880335
>100	0.0071408517367646165	0.8205960063867707
>500	2.8947649953373484E-4	0.17955273710953717
>1k	4.824608325562248E-4	0.7746337583340841
>5k	1.929843330224899E-4	0.8695875008306146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6601	0.4647007639649584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5744	0.40436921499995776	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4419	0.3110911492139299	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2880	0.20274779582170585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.039854021587008E-5	0.0	0.0	0.0	0.0
3	7.039854021587008E-5	0.0	0.0	0.0	0.0
4	7.039854021587008E-5	0.0	0.0	0.0	0.0
5	7.039854021587008E-5	0.0	0.0	0.0	0.0
6	7.039854021587008E-5	0.0	0.0	0.0	7.039854021587008E-5
7	7.039854021587008E-5	0.0	0.0	0.0	7.039854021587008E-5
8	1.4079708043174017E-4	0.0	0.0	0.0	7.039854021587008E-5
9	1.4079708043174017E-4	0.0	0.0	0.0	7.039854021587008E-5
10	1.4079708043174017E-4	0.0	0.0	7.039854021587008E-5	7.039854021587008E-5
11	1.4079708043174017E-4	0.0	0.0	1.4079708043174017E-4	7.039854021587008E-5
12	1.4079708043174017E-4	0.0	0.0	1.4079708043174017E-4	7.039854021587008E-5
13	1.4079708043174017E-4	0.0	0.0	1.4079708043174017E-4	7.039854021587008E-5
14	2.1119562064761025E-4	0.0	0.0	2.1119562064761025E-4	1.4079708043174017E-4
15	2.1119562064761025E-4	0.0	0.0	2.8159416086348033E-4	1.4079708043174017E-4
16	2.1119562064761025E-4	0.0	0.0	5.631883217269607E-4	2.1119562064761025E-4
17	2.1119562064761025E-4	0.0	0.0	6.335868619428308E-4	2.1119562064761025E-4
18	2.1119562064761025E-4	0.0	0.0	7.039854021587008E-4	2.1119562064761025E-4
19	2.1119562064761025E-4	0.0	0.0	9.151810228063111E-4	2.1119562064761025E-4
20	2.1119562064761025E-4	0.0	0.0	0.0013375722641015316	2.8159416086348033E-4
21	2.1119562064761025E-4	0.0	0.0	0.0021823547466919726	2.8159416086348033E-4
22	2.1119562064761025E-4	0.0	0.0	0.003379129930361764	2.8159416086348033E-4
23	2.1119562064761025E-4	0.0	0.0	0.0052094919759743865	5.631883217269607E-4
24	2.1119562064761025E-4	0.0	0.0	0.00844782482590441	5.631883217269607E-4
25	2.1119562064761025E-4	0.0	0.0	0.011967751836697915	5.631883217269607E-4
26	2.1119562064761025E-4	0.0	0.0	0.01795162775504687	5.631883217269607E-4
27	2.1119562064761025E-4	0.0	0.0	0.049208579610893186	5.631883217269607E-4
28	2.1119562064761025E-4	0.0	0.0	0.13643237093835622	5.631883217269607E-4
29	2.1119562064761025E-4	0.0	0.0	0.2535051433173482	6.335868619428308E-4
30	2.1119562064761025E-4	0.0	0.0	0.40211646171304993	6.335868619428308E-4
31	2.1119562064761025E-4	0.0	0.0	0.8378834256492858	7.039854021587008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2375	0.0	19.863157	1
CTTATAC	1420	0.0	17.197184	37
GGTTAGT	115	1.2426226E-6	16.086956	1
CGTAGAC	70	0.0025928973	15.857143	3
GTACTAG	100	1.09365195E-4	14.8	1
GTATTAC	125	2.9597504E-6	14.799999	1
GTATCAA	3290	0.0	14.395136	2
TCTTATA	2235	0.0	13.90604	37
GTCCTAC	205	1.4242687E-9	13.536585	1
GTACTAA	115	3.5780916E-4	12.869565	1
TACGGAT	395	0.0	12.64557	27
GAGTGTC	440	0.0	12.613636	7
TATACTG	220	4.08545E-9	12.613636	5
GGAGTGT	535	0.0	12.448599	6
GCACCCG	605	0.0	12.231405	9
CTAGAGT	215	4.0126906E-8	12.046512	4
TGTATAC	170	5.3104304E-6	11.970589	3
GTATAAT	140	1.398749E-4	11.892858	1
GTAAGGT	140	1.398749E-4	11.892858	4
GTAAGAG	640	0.0	11.8515625	3
>>END_MODULE