##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3896001
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33927352688051	31.0	31.0	33.0	30.0	34.0
2	31.595268584376647	31.0	31.0	34.0	30.0	34.0
3	31.643171549493957	31.0	31.0	34.0	30.0	34.0
4	35.41669676162814	37.0	35.0	37.0	33.0	37.0
5	35.235333615160776	37.0	35.0	37.0	32.0	37.0
6	35.34457665693618	37.0	35.0	37.0	33.0	37.0
7	35.288615942347036	37.0	35.0	37.0	32.0	37.0
8	35.274999929414804	37.0	35.0	37.0	32.0	37.0
9	36.892304699100436	39.0	37.0	39.0	33.0	39.0
10	36.66277575390766	38.0	35.0	39.0	32.0	39.0
11	36.77793691531393	38.0	37.0	39.0	32.0	39.0
12	36.5774315766346	38.0	35.0	39.0	32.0	39.0
13	36.645206969916075	38.0	35.0	39.0	32.0	39.0
14	37.68001650923601	39.0	37.0	41.0	32.0	41.0
15	37.66125316702947	39.0	37.0	41.0	32.0	41.0
16	37.57348085896282	39.0	36.0	41.0	32.0	41.0
17	37.493200592094304	39.0	36.0	40.0	32.0	41.0
18	37.39064440691879	39.0	36.0	40.0	32.0	41.0
19	37.36718317064087	39.0	36.0	40.0	32.0	41.0
20	37.26236158563614	39.0	36.0	40.0	31.0	41.0
21	37.148778965919156	39.0	36.0	40.0	31.0	41.0
22	37.04524896169175	39.0	36.0	40.0	31.0	41.0
23	36.916937392983215	39.0	36.0	40.0	31.0	41.0
24	36.78871771336814	39.0	36.0	40.0	30.0	41.0
25	37.07202231210926	39.0	36.0	40.0	31.0	41.0
26	37.0067977395283	39.0	36.0	40.0	31.0	41.0
27	36.94357290976055	39.0	36.0	40.0	31.0	41.0
28	36.876680986478185	39.0	36.0	40.0	30.0	41.0
29	36.79388814325253	39.0	36.0	40.0	30.0	41.0
30	36.694296536371525	39.0	36.0	40.0	30.0	41.0
31	36.595567865614	39.0	35.0	40.0	30.0	41.0
32	36.48196419867449	39.0	35.0	40.0	30.0	41.0
33	36.395180083372665	38.0	35.0	40.0	30.0	41.0
34	36.28813775971823	38.0	35.0	40.0	30.0	41.0
35	36.1610459032223	38.0	35.0	40.0	29.0	41.0
36	36.067742025733565	38.0	35.0	40.0	29.0	41.0
37	35.986203802309085	38.0	35.0	40.0	29.0	41.0
38	35.88159037946859	38.0	35.0	40.0	28.0	41.0
39	35.72830602456211	38.0	34.0	40.0	27.0	41.0
40	35.60110328513776	38.0	34.0	40.0	27.0	41.0
41	35.50371419309184	38.0	34.0	40.0	27.0	41.0
42	35.3458733711824	38.0	34.0	40.0	26.0	41.0
43	35.197075154754835	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	7.0
14	15.0
15	41.0
16	92.0
17	181.0
18	446.0
19	833.0
20	1742.0
21	3299.0
22	5762.0
23	9083.0
24	14506.0
25	21895.0
26	31509.0
27	42919.0
28	57946.0
29	76592.0
30	98217.0
31	123028.0
32	152922.0
33	189500.0
34	239322.0
35	305738.0
36	410208.0
37	593299.0
38	918367.0
39	598524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99316221941422	21.538187490198283	13.709929745911257	24.758720544476244
2	22.820605025512055	23.10369016845735	30.26164521005	23.814059595980595
3	23.490086373181114	23.488469330475017	28.377713455412355	24.64373084093151
4	19.21747453350243	17.489189556162845	30.914827793935373	32.37850811639935
5	16.34398964476652	35.74023723299866	32.64421646709023	15.271556655144597
6	36.36813748251092	34.40527864340897	13.8528711876614	15.373712686418713
7	30.048195572845078	29.930331126711724	19.970400418275048	20.051072882168153
8	27.854587306317423	33.55420083311067	18.629743678197208	19.9614681823747
9	26.997683008808266	13.678692587604571	18.27468730115829	41.04893710242887
10	17.15240832843729	27.204228130331586	32.533359206016634	23.110004335214494
11	37.41821421503742	21.068552087127287	20.122197093891916	21.391036603943377
12	22.08451691875849	24.867729756742875	28.732282152904993	24.315471171593643
13	31.855638640749834	19.559928244371598	23.09545095085961	25.488982164018953
14	22.809978744871987	20.393449591003698	24.545373576649492	32.251198087474826
15	26.057488178262787	27.50615310417015	21.51341850271599	24.922940214851074
16	25.316240935256435	25.67532708538833	23.84234500966504	25.166086969690205
17	24.812493631290135	25.72943384768125	24.761980297232984	24.69609222379563
18	25.11547096625489	24.74380781729779	25.25271425751687	24.888006958930454
19	25.82409501434933	24.62922879126571	25.6893157881633	23.857360406221662
20	25.521682360964487	24.33700607366374	25.64693900232572	24.494372563046056
21	25.455640283459886	24.47096394482445	25.49208791270844	24.58130785900722
22	25.412390807907904	24.565548109458906	25.19385903648382	24.82820204614937
23	25.26241651375346	24.448222677560917	25.166625983925567	25.122734824760055
24	25.00818146607252	24.683925902483082	25.158284096949668	25.149608534494728
25	25.458720364804833	24.408797636345575	25.379279933449713	24.753202065399883
26	25.12265782272643	24.716626099428616	25.26431589724951	24.896400180595435
27	25.358027372169566	24.85410039679148	25.17032208153951	24.617550149499447
28	24.99221637776787	24.964880655831454	25.291908292631344	24.750994673769334
29	25.077945308535597	25.028869345772755	25.342421626688495	24.55076371900315
30	25.325096169123164	24.873299570508323	25.12591757548317	24.67568668488535
31	25.317703973895284	24.501508084828522	25.43164131631383	24.749146624962364
32	24.964700984419665	24.833643523192116	25.18025534387697	25.02140014851126
33	24.992139375734247	24.50833559847649	25.37057870365023	25.128946322139033
34	25.309233750196675	24.613315037650143	25.410157748932814	24.667293463220364
35	25.27922862442797	24.894783137889338	25.238725554741904	24.58726268294079
36	25.149069520259363	24.85931086773335	25.258951422240393	24.73266818976689
37	25.27422349224243	24.838006971764123	25.254998651181044	24.632770884812402
38	25.03864860404297	24.817473096131135	25.439906201256107	24.70397209856979
39	24.935388876953574	25.13821223351842	25.342241955276705	24.5841569342513
40	25.280075646797833	24.83664660250344	25.46251913179694	24.420758618901793
41	25.068936070601627	24.812082953777477	25.367216281515326	24.75176469410557
42	25.070886788786755	24.864829346809717	25.09629745988258	24.967986404520943
43	25.16893604493428	24.654613795016996	25.220142397294047	24.956307762754683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	185.0
1	276.0
2	367.0
3	556.5
4	746.0
5	746.0
6	1306.0
7	1866.0
8	1700.5
9	1535.0
10	2261.0
11	2987.0
12	2987.0
13	7056.5
14	11126.0
15	16858.0
16	22590.0
17	19504.5
18	16419.0
19	16419.0
20	21374.0
21	26329.0
22	22527.0
23	18725.0
24	22481.5
25	26238.0
26	26238.0
27	32808.5
28	39379.0
29	47593.0
30	55807.0
31	66388.5
32	76970.0
33	76970.0
34	89732.0
35	102494.0
36	119304.5
37	136115.0
38	154565.5
39	173016.0
40	173016.0
41	191720.5
42	210425.0
43	232406.0
44	254387.0
45	271976.0
46	289565.0
47	289565.0
48	307792.5
49	326020.0
50	334569.5
51	343119.0
52	342497.0
53	341875.0
54	341875.0
55	329552.0
56	317229.0
57	296149.0
58	275069.0
59	252384.0
60	229699.0
61	229699.0
62	204167.5
63	178636.0
64	154378.0
65	130120.0
66	110935.5
67	91751.0
68	91751.0
69	79008.5
70	66266.0
71	57127.0
72	47988.0
73	39553.5
74	31119.0
75	31119.0
76	25828.5
77	20538.0
78	16581.0
79	12624.0
80	9910.5
81	7197.0
82	7197.0
83	5710.5
84	4224.0
85	3516.5
86	2809.0
87	2126.5
88	1444.0
89	1444.0
90	1041.0
91	638.0
92	435.5
93	233.0
94	167.5
95	102.0
96	102.0
97	64.0
98	26.0
99	15.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3896001.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.28849353623382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29026605363418	47.60288919943385
2	10.510255479102952	12.46274428073923
3	3.5903985310188116	6.386079602964367
4	1.6882548498634367	4.003763470145751
5	0.9593036329936718	2.843783362201547
6	0.6506641635923827	2.314613883444358
7	0.4124309009788994	1.7116684764781394
8	0.3091719449643969	1.466427108848519
9	0.2371946020176654	1.265661956569852
>10	1.2578446659238416	13.28131519305591
>50	0.06727900180910501	2.69763784912103
>100	0.025768444092118297	2.561884313904959
>500	8.217359603809408E-4	0.30059923083209833
>1k	2.1624628007814732E-4	0.2689058959309554
>5k	4.324925601562799E-5	0.17487729667675064
>10k+	8.649851203125597E-5	0.6571488796527516
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13422	0.3445070984324696	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12206	0.31329560747032664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6820	0.17505128977122952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4778	0.12263857221802561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1334689082472E-5	0.0	0.0	0.0	1.02669378164944E-4
2	4.62012201742248E-4	0.0	0.0	2.5667344541236E-5	1.02669378164944E-4
3	6.67350958072136E-4	2.5667344541236E-5	0.0	2.5667344541236E-5	1.02669378164944E-4
4	6.93018302613372E-4	2.5667344541236E-5	0.0	2.5667344541236E-5	1.02669378164944E-4
5	0.002156056941463824	2.5667344541236E-5	0.0	1.79671411788652E-4	1.02669378164944E-4
6	0.0029517446222421403	2.5667344541236E-5	0.0	3.59342823577304E-4	1.2833672270618E-4
7	0.003362422134901916	2.5667344541236E-5	0.0	3.8501016811854E-4	1.2833672270618E-4
8	0.0043634485720101205	2.5667344541236E-5	0.0	4.62012201742248E-4	1.2833672270618E-4
9	0.0044917852947163	2.5667344541236E-5	0.0	5.1334689082472E-4	1.54004067247416E-4
10	0.006981517715216192	2.5667344541236E-5	0.0	7.95687680778316E-4	1.54004067247416E-4
11	0.007469197261499676	2.5667344541236E-5	0.0	8.72689714402024E-4	1.54004067247416E-4
12	0.007879874774159452	2.5667344541236E-5	0.0	0.00102669378164944	1.54004067247416E-4
13	0.008495891043149116	2.5667344541236E-5	0.0	0.00115503050435562	1.54004067247416E-4
14	0.009342913413009904	2.5667344541236E-5	0.0	0.001309034571603036	2.31006100871124E-4
15	0.00988192764837586	2.5667344541236E-5	0.0	0.001488705983391688	2.82340789953596E-4
16	0.010420941883741817	2.5667344541236E-5	0.0	0.001745379428804048	3.08008134494832E-4
17	0.010497943917365525	2.5667344541236E-5	0.0	0.00179671411788652	3.08008134494832E-4
18	0.010677615329154176	2.5667344541236E-5	0.0	0.0018223814624277561	3.08008134494832E-4
19	0.011190962219978897	2.5667344541236E-5	0.0	0.0019250508405927	3.33675479036068E-4
20	0.014322378254009688	2.5667344541236E-5	0.0	0.002772073210453488	3.59342823577304E-4
21	0.021149891901978465	2.5667344541236E-5	0.0	0.004517452639257536	3.8501016811854E-4
22	0.021560569414638242	2.5667344541236E-5	0.0	0.00474845874012866	3.8501016811854E-4
23	0.021971246927298016	2.5667344541236E-5	0.0	0.004979464840999784	4.10677512659776E-4
24	0.023023608053488694	2.5667344541236E-5	0.0	0.005364475009118324	4.10677512659776E-4
25	0.023639624322478358	5.1334689082472E-5	0.0	0.005672483143613156	4.62012201742248E-4
26	0.025975352675730833	5.1334689082472E-5	0.0	0.007161189127004844	4.62012201742248E-4
27	0.02651436691109679	5.1334689082472E-5	0.0	0.00808521353048934	4.62012201742248E-4
28	0.027772066793617354	5.1334689082472E-5	0.0	0.010035931715623276	4.62012201742248E-4
29	0.028003072894488477	5.1334689082472E-5	0.0	0.012910674304241709	4.62012201742248E-4
30	0.028336748373524547	5.1334689082472E-5	0.0	0.01617042706097868	4.62012201742248E-4
31	0.029312107466091513	5.1334689082472E-5	0.0	0.025487673129447348	4.62012201742248E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5285	0.0	18.412489	1
GTATCAA	6865	0.0	14.147851	2
GGGTATA	355	0.0	11.985915	1
ACGCGTA	145	1.9404772E-4	11.4827585	29
TAGACTG	640	0.0	10.984375	5
TTAGTAC	220	7.01968E-7	10.931818	3
TAATCCC	1525	0.0	10.918033	5
TAGGTTG	395	0.0	10.772152	5
TTAATCC	1605	0.0	10.719626	4
TTTAATC	1815	0.0	10.600552	3
CTAATAC	705	0.0	10.496454	3
TAGTACT	230	1.2439432E-6	10.456522	4
TATCAAC	9520	0.0	10.415967	3
GGACCGT	320	8.6765795E-10	10.40625	6
TAGGACT	570	0.0	10.385964	4
TAGTACA	340	2.2737368E-10	10.338235	4
ACTATAC	435	0.0	10.206896	3
CTACACT	755	0.0	10.046357	4
ATCAACG	9805	0.0	10.037736	2
AACGCAG	9870	0.0	10.009119	5
>>END_MODULE