FastQCFastQC Report
Thu 9 Feb 2017
SRR2727595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9489877
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT687230.7241716620773905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT628920.662727240827252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT451690.4759703418706059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217630.229328578231309No Hit
GGCAGAGGCAGAGGCGATTCGGTCGCCGACGCTGGGGCTGACG118300.12465914995526284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA255050.020.2734761
TTAATCC50800.014.2027564
TTTAATC49650.014.1218533
GTATCAA374700.013.8145182
TAATCCC49900.013.7174365
TAATACT15150.013.6765684
CGCAATC6100.013.04098322
AACGACG7550.012.98675415
TATACCG3650.012.6712335
AATACTG15950.012.4106595
CCTTTAA58100.012.3227191
CTTTAAT57850.012.1840962
CGTGCGA11700.011.70085517
GTACGAC1901.4369161E-611.6842113
GTGCGAG11600.011.64224218
TATACAC15400.011.2922083
CTAATAC19050.011.2650923
CGCAATA3950.011.24050636
GGCGGGT10750.011.18604633
AATCCCA70650.010.919326
Produced by FastQC (version 0.11.5)