FastQCFastQC Report
Thu 9 Feb 2017
SRR2727594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688095
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT515570.532168604870204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT514560.5311260882557406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT339960.3509048992603809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186040.19202949599482663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220450.019.3853471
CGCTTCG14300.015.13636432
GTATCAA310850.013.7180322
GACCGAT25350.013.57396519
TAAACGC15950.013.45454528
ATCTCCG16650.013.44444510
CACGAGA17050.013.12903221
ACGTCAG26550.013.03013230
GCACGTC26450.013.00945128
GTAAACG16650.013.027
CGCTACG18600.012.83064510
TATACAC26700.012.8183523
CCGATTT27000.012.74444421
CGATTTG27400.012.69343122
TTGCACG27350.012.51371126
ACCGATT27650.012.51175420
ACGAGAC18100.012.46961322
CGAGACT18550.012.46630723
CCACGAG19200.012.42968720
ACGCTTC17700.012.22881331
Produced by FastQC (version 0.11.5)