##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9688095
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665763186673953	31.0	31.0	34.0	30.0	34.0
2	31.865806435630535	31.0	31.0	34.0	30.0	34.0
3	31.92894217077764	31.0	31.0	34.0	30.0	34.0
4	35.637353989613025	37.0	35.0	37.0	33.0	37.0
5	35.52650144326619	37.0	35.0	37.0	33.0	37.0
6	35.57555752704737	37.0	35.0	37.0	33.0	37.0
7	35.493188598997016	37.0	35.0	37.0	33.0	37.0
8	35.470389276736036	37.0	35.0	37.0	33.0	37.0
9	37.101566819895964	39.0	37.0	39.0	33.0	39.0
10	36.9424028150013	39.0	37.0	39.0	33.0	39.0
11	37.0221707157083	39.0	37.0	39.0	33.0	39.0
12	36.85180595359562	39.0	37.0	39.0	32.0	39.0
13	36.88900139810768	39.0	37.0	39.0	33.0	39.0
14	37.96585912916832	40.0	37.0	41.0	33.0	41.0
15	37.96437792982005	40.0	37.0	41.0	33.0	41.0
16	37.8828050302975	40.0	37.0	41.0	33.0	41.0
17	37.85398202639425	40.0	37.0	41.0	32.0	41.0
18	37.77547113235368	40.0	37.0	41.0	32.0	41.0
19	37.77634860104076	40.0	37.0	41.0	32.0	41.0
20	37.68635670892988	40.0	37.0	41.0	32.0	41.0
21	37.57624032382011	39.0	37.0	41.0	32.0	41.0
22	37.475547566368824	39.0	36.0	41.0	32.0	41.0
23	37.356136887592456	39.0	36.0	41.0	31.0	41.0
24	37.22923433347836	39.0	36.0	41.0	31.0	41.0
25	37.48626876594418	39.0	36.0	41.0	32.0	41.0
26	37.41357779831845	39.0	36.0	41.0	31.0	41.0
27	37.34990305111583	39.0	36.0	41.0	31.0	41.0
28	37.26798023760089	39.0	36.0	41.0	31.0	41.0
29	37.183625676668115	39.0	36.0	41.0	31.0	41.0
30	37.06884366844049	39.0	36.0	41.0	31.0	41.0
31	36.958957359522174	39.0	36.0	41.0	30.0	41.0
32	36.83715013116614	39.0	36.0	41.0	30.0	41.0
33	36.74110317869509	39.0	35.0	41.0	30.0	41.0
34	36.62251206248494	39.0	35.0	41.0	30.0	41.0
35	36.475793022260824	39.0	35.0	41.0	30.0	41.0
36	36.38617261701088	39.0	35.0	40.0	30.0	41.0
37	36.30126748344231	39.0	35.0	40.0	29.0	41.0
38	36.18415096053455	39.0	35.0	40.0	29.0	41.0
39	36.03857941112262	39.0	35.0	40.0	28.0	41.0
40	35.895948481099744	38.0	35.0	40.0	27.0	41.0
41	35.782349471180865	38.0	35.0	40.0	27.0	41.0
42	35.61874610023952	38.0	34.0	40.0	27.0	41.0
43	35.46764518721173	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	4.0
13	13.0
14	27.0
15	80.0
16	227.0
17	516.0
18	1019.0
19	2121.0
20	4165.0
21	7719.0
22	13318.0
23	21454.0
24	33248.0
25	49424.0
26	71149.0
27	98220.0
28	133064.0
29	175127.0
30	226547.0
31	283754.0
32	351087.0
33	434557.0
34	543739.0
35	682689.0
36	887504.0
37	1243684.0
38	1941285.0
39	2482350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73506762681415	19.601985736101884	12.842772495521565	25.8201741415624
2	20.478680277185557	22.67621240295435	32.55598752902402	24.28911979083607
3	20.745223906247823	23.227156628831573	30.210521263468205	25.8170982014524
4	15.42835820664434	17.32075294472236	33.67655870426539	33.574330144367906
5	15.385119572010803	36.08643391709103	33.36040779946935	15.16803871142882
6	34.34130239226597	35.487244912441504	14.693848481048132	15.477604214244389
7	29.21677584705765	30.75482847763157	20.335659384017188	19.692736291293592
8	27.501598611491733	33.73233850411252	18.971211574618128	19.79485130977762
9	27.133600568532824	13.704500213922344	18.917021354559385	40.24487786298545
10	16.910228481450687	27.823374977227207	32.45073463874993	22.815661902572177
11	36.22596599228228	21.690807119459503	20.457891876576355	21.625335011681862
12	22.09062772402624	26.373471771282176	29.048827452662263	22.48707305202932
13	32.173982604423266	20.10186729176376	23.240100349965605	24.484049753847376
14	22.161807868316732	21.600789422481924	26.006991054484914	30.230411654716434
15	25.8891144234238	27.82575934690979	22.429332082313397	23.855794147353016
16	24.130172133943773	26.28281411361057	24.94882636885786	24.638187383587795
17	24.4220458201535	25.78333511386914	25.306554074872306	24.488064991105063
18	24.496704460474426	24.54194555276347	26.34146341463415	24.619886572127957
19	25.472819991959206	24.519319845645608	26.793234376830533	23.214625785564653
20	25.137976041729566	24.51185707819752	26.919575004167484	23.430591875905428
21	24.800654824297244	24.955411770838335	26.69220316274768	23.55173024211674
22	24.57870200488331	25.324173637851406	26.221295311410554	23.875829045854733
23	24.452815543200185	24.856197219370785	26.187594155507355	24.50339308192168
24	23.760047769969226	25.399534170546428	26.69684803875272	24.14357002073163
25	24.282967910616073	24.942705454477892	26.684688785566202	24.089637849339834
26	23.93504605394559	25.418970396140832	26.514118616714637	24.13186493319894
27	24.73442921441212	25.271139475820583	26.513354792660476	23.481076517106818
28	23.910459176958938	25.355480102125338	26.525111489926555	24.20894923098917
29	24.10742256346578	25.46609008272524	26.446489222081325	23.97999813172765
30	24.040433129526495	25.580333388555747	26.467876295597843	23.91135718631991
31	24.34502345404334	24.840445928740376	26.518990575546585	24.2955400416697
32	23.75468035769674	25.512507876935558	26.338294577004046	24.394517188363658
33	23.91055207447904	24.8771198052868	26.87339461473076	24.338933505503405
34	24.28864498128889	25.34772832017027	26.790457773174193	23.573168925366648
35	24.414159852891615	25.656148086904594	26.486342258204527	23.443349801999258
36	23.941249543898984	25.474265064494105	26.704888835214767	23.879596556392148
37	24.289646210116643	25.564891756325675	25.921380828738776	24.224081204818905
38	23.820792426168406	25.388799346001456	26.801915133986608	23.988493093843527
39	24.12141912316095	25.456181013914502	26.528300971450015	23.894098891474535
40	24.320219816176454	25.19417904139049	26.81249512933141	23.67310601310165
41	24.038121013470658	25.232648936658858	26.774737448383817	23.95449260148667
42	23.78822668439977	25.282792953619882	26.47334692733711	24.45563343464324
43	23.89065136128413	24.961315924338066	26.68427590769909	24.463756806678713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	688.0
2	1110.0
3	2533.0
4	3956.0
5	3956.0
6	6049.5
7	8143.0
8	7508.5
9	6874.0
10	9977.5
11	13081.0
12	13081.0
13	28183.0
14	43285.0
15	68353.0
16	93421.0
17	83901.0
18	74381.0
19	74381.0
20	88643.0
21	102905.0
22	86245.0
23	69585.0
24	82784.0
25	95983.0
26	95983.0
27	115424.0
28	134865.0
29	161210.5
30	187556.0
31	217825.0
32	248094.0
33	248094.0
34	280579.0
35	313064.0
36	350007.5
37	386951.0
38	425118.5
39	463286.0
40	463286.0
41	515221.5
42	567157.0
43	608931.0
44	650705.0
45	687883.0
46	725061.0
47	725061.0
48	763917.5
49	802774.0
50	812899.0
51	823024.0
52	801359.5
53	779695.0
54	779695.0
55	734452.0
56	689209.0
57	636077.5
58	582946.0
59	521679.0
60	460412.0
61	460412.0
62	406272.0
63	352132.0
64	302660.0
65	253188.0
66	223745.0
67	194302.0
68	194302.0
69	174210.5
70	154119.0
71	139099.0
72	124079.0
73	104338.5
74	84598.0
75	84598.0
76	75604.5
77	66611.0
78	58173.5
79	49736.0
80	40633.0
81	31530.0
82	31530.0
83	25796.0
84	20062.0
85	17594.0
86	15126.0
87	12056.0
88	8986.0
89	8986.0
90	6414.0
91	3842.0
92	2585.0
93	1328.0
94	916.0
95	504.0
96	504.0
97	315.0
98	126.0
99	81.5
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9688095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.25002268889423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7737485308335	43.522513936177994
2	13.186737996396191	14.571351469867865
3	3.7995168937050043	6.297701837521153
4	1.4752986070534049	3.2604112605037865
5	0.751538885842807	2.0761270247200687
6	0.4248214228701843	1.4082835951383605
7	0.2827823518682912	1.0936611949719373
8	0.1901746689269256	0.840572381845247
9	0.13799484774994034	0.6861796622221237
>10	0.8248757856281661	8.57824314773401
>50	0.08210636351180053	3.150129142056509
>100	0.05995256469639223	6.584685508665086
>500	0.006652167403599919	2.5640558816224988
>1k	0.003631314511397245	3.442854543748436
>5k	9.311055670319352E-5	0.3221707125583622
>10k+	7.448844536255481E-5	1.6010587006465182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51557	0.532168604870204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	51456	0.5311260882557406	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33996	0.3509048992603809	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18604	0.19202949599482663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0643893355711315E-5	0.0	0.0	0.0	1.0321946677855657E-5
2	6.296387473491951E-4	0.0	0.0	2.0643893355711315E-5	1.0321946677855657E-5
3	9.186532543291535E-4	0.0	0.0	2.0643893355711315E-5	1.0321946677855657E-5
4	0.0010115507744298544	0.0	0.0	2.0643893355711315E-5	1.0321946677855657E-5
5	0.0036230032839273357	0.0	0.0	1.5482920016783485E-4	1.0321946677855657E-5
6	0.005357090325807086	0.0	0.0	2.683706136242471E-4	2.0643893355711315E-5
7	0.006089948539934838	0.0	0.0	3.096584003356697E-4	2.0643893355711315E-5
8	0.007824035581814588	0.0	0.0	4.4384370714779326E-4	2.0643893355711315E-5
9	0.00848464016919735	0.0	0.0	4.954534405370715E-4	3.096584003356697E-5
10	0.0124069799067825	0.0	0.0	8.257557342284525E-4	3.096584003356697E-5
11	0.013480462361279488	0.0	0.0	8.980093609734422E-4	4.128778671142263E-5
12	0.014120423055306539	0.0	0.0	9.289752010070091E-4	5.160973338927828E-5
13	0.015183583563125672	0.0	0.0	0.0010734824544969884	5.160973338927828E-5
14	0.017320226525441792	0.0	0.0	0.0013934628015105136	9.289752010070091E-5
15	0.018858196580442285	0.0	0.0	0.001537970055000493	1.0321946677855656E-4
16	0.02003489850171783	0.0	0.0	0.0016721553618126164	1.1354141345641223E-4
17	0.020334234955375643	0.0	0.0	0.0017031212018461834	1.1354141345641223E-4
18	0.020860654235946283	0.0	0.0	0.00180634066862474	1.3418530681212355E-4
19	0.021810273330309004	0.0	0.0	0.001981813762148286	1.3418530681212355E-4
20	0.028240846110613078	0.0	0.0	0.0030552962166452745	1.6515114684569052E-4
21	0.04266060561957743	0.0	0.0	0.005130007498894262	1.8579504020140182E-4
22	0.04365151250065157	0.0	0.0	0.005480953685941353	1.8579504020140182E-4
23	0.044683707168437135	0.0	0.0	0.005769968192921312	2.5804866694639143E-4
24	0.04639715031696118	0.0	0.0	0.00627574358013624	2.786925603021027E-4
25	0.04744998887810246	0.0	0.0	0.0065028264070490635	2.890145069799584E-4
26	0.05432440536555432	0.0	0.0	0.008092406195438835	2.890145069799584E-4
27	0.05575915595377626	0.0	0.0	0.00959941041040576	2.890145069799584E-4
28	0.0587525204903544	0.0	0.0	0.013408208734534499	2.890145069799584E-4
29	0.059113788624079344	0.0	0.0	0.021665766076819023	2.890145069799584E-4
30	0.05994986630498565	0.0	0.0	0.03370115590319872	2.9933645365781404E-4
31	0.06150848025334186	0.0	0.0	0.06311870393508734	2.9933645365781404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22045	0.0	19.385347	1
CGCTTCG	1430	0.0	15.136364	32
GTATCAA	31085	0.0	13.718032	2
GACCGAT	2535	0.0	13.573965	19
TAAACGC	1595	0.0	13.454545	28
ATCTCCG	1665	0.0	13.444445	10
CACGAGA	1705	0.0	13.129032	21
ACGTCAG	2655	0.0	13.030132	30
GCACGTC	2645	0.0	13.009451	28
GTAAACG	1665	0.0	13.0	27
CGCTACG	1860	0.0	12.830645	10
TATACAC	2670	0.0	12.818352	3
CCGATTT	2700	0.0	12.744444	21
CGATTTG	2740	0.0	12.693431	22
TTGCACG	2735	0.0	12.513711	26
ACCGATT	2765	0.0	12.511754	20
ACGAGAC	1810	0.0	12.469613	22
CGAGACT	1855	0.0	12.466307	23
CCACGAG	1920	0.0	12.429687	20
ACGCTTC	1770	0.0	12.228813	31
>>END_MODULE