FastQCFastQC Report
Thu 9 Feb 2017
SRR2727593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8538644
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204150.23908948540306865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT192150.2250357316688692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137420.1609389031794744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93900.016.7859421
TAATCCC31000.012.23387055
GTATCAA128900.012.2137322
TTAATCC32700.011.6544354
ATACCGT4050.010.9629636
GTATAGT9500.010.9052631
TATACTG11500.010.778265
TCGTTAC9800.010.57142823
CCTTTAA39950.010.5581981
GTAATAC5800.010.5258623
GTATAGA7750.010.2645161
GTATACG2208.319323E-610.0909091
GTCTTAG12500.010.0641
AACGCAG178700.010.0523225
TTACACA16100.09.9968954
TATCAAC178000.09.7800571
GGGTATA10050.09.7562191
TTTAATC39450.09.754123
CTACACT17350.09.703174
GTATAGC17850.09.6386561
Produced by FastQC (version 0.11.5)