FastQCFastQC Report
Thu 9 Feb 2017
SRR2727592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9428502
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT473520.50222187999748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT466360.49462788468412056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302530.3208675142668475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195810.2076788019984511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239200.013.743521
CGCGTAA1552.7245595E-511.93548410
GGACCGT7400.011.56
TTAATCC25950.011.05009654
CGTATAC2207.0237E-710.9318183
GTACTAT5700.010.7105261
GTATCAA314400.010.432732
TAATCCC25900.010.4285725
TACACCG5250.010.2190485
AACGCAG337150.010.0415255
GTATAGG14000.09.9107141
TCTACAC18900.09.8862443
TAGATCG4351.8189894E-129.781615
GGGTAAG31050.09.77133751
TCGTTAC10350.09.65217323
TATACTG12450.09.510045
CGCGTAT3701.0550139E-99.57
GTATTAG12200.09.401641
GTAAGAG34850.09.3959833
GTATTAT11650.09.3690991
Produced by FastQC (version 0.11.5)