FastQCFastQC Report
Thu 9 Feb 2017
SRR2727591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5207159
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547261.050976165698032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT526761.0116072891186922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238980.458945079264912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219650.42182310930010014No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79530.15273203679780087No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70890.13613949564436192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279000.012.5521511
ATACTGT10800.011.1342596
TAGACAG8050.010.8012435
CGTACGT1200.00716809610.79166710
TATATCG1553.600789E-410.7419364
AACGCAG380200.010.64650255
TATACTG7550.010.5364255
TTACACT7050.010.4964544
GTATAGA3554.5474735E-1110.4225351
TACACTC7650.010.3986935
TACGCTG3953.6379788E-1210.3037985
GTATCAA341800.010.2783792
TTACACC9000.010.2777784
GGGTAAG29950.010.1919861
TAAGGTG6800.010.0661765
CTACACT11050.010.0452484
TTTACAC8000.09.943753
TACTATA8100.09.5925922
GTTACAC4850.09.5360823
ATACCGT3504.083631E-99.5142866
Produced by FastQC (version 0.11.5)