##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727591.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5207159
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153889673812532	31.0	31.0	33.0	30.0	34.0
2	31.38694401304051	31.0	31.0	34.0	30.0	34.0
3	31.378554793506403	31.0	31.0	34.0	28.0	34.0
4	35.18617100034779	37.0	35.0	37.0	32.0	37.0
5	35.017836789696645	35.0	35.0	37.0	32.0	37.0
6	35.16848189194914	37.0	35.0	37.0	32.0	37.0
7	35.10339668905827	37.0	35.0	37.0	32.0	37.0
8	35.104250129485195	37.0	35.0	37.0	32.0	37.0
9	36.68310819777157	38.0	35.0	39.0	32.0	39.0
10	36.41246349496914	38.0	35.0	39.0	32.0	39.0
11	36.575593524223095	38.0	35.0	39.0	32.0	39.0
12	36.36814892727493	38.0	35.0	39.0	32.0	39.0
13	36.45453403669832	38.0	35.0	39.0	32.0	39.0
14	37.36119465528132	39.0	36.0	40.0	32.0	41.0
15	37.375671839480994	39.0	36.0	40.0	32.0	41.0
16	37.25041082863035	39.0	36.0	40.0	32.0	41.0
17	37.24167132211634	39.0	36.0	40.0	32.0	41.0
18	37.17777717177447	39.0	36.0	40.0	31.0	41.0
19	37.17958641170742	39.0	36.0	40.0	31.0	41.0
20	37.09614244542946	39.0	36.0	40.0	31.0	41.0
21	36.99088620109353	39.0	36.0	40.0	31.0	41.0
22	36.88473522702111	39.0	36.0	40.0	31.0	41.0
23	36.768160526690274	39.0	36.0	40.0	30.0	41.0
24	36.64171825749896	38.0	35.0	40.0	30.0	41.0
25	36.904993682735636	39.0	36.0	40.0	31.0	41.0
26	36.789367676308714	39.0	36.0	40.0	30.0	41.0
27	36.70968507011213	39.0	35.0	40.0	30.0	41.0
28	36.609560222762546	39.0	35.0	40.0	30.0	41.0
29	36.509827335789055	39.0	35.0	40.0	30.0	41.0
30	36.377803328071984	38.0	35.0	40.0	30.0	41.0
31	36.22213341286486	38.0	35.0	40.0	30.0	41.0
32	36.090080214566136	38.0	35.0	40.0	30.0	41.0
33	35.96737126713435	38.0	35.0	40.0	29.0	41.0
34	35.84662980331501	38.0	35.0	40.0	29.0	41.0
35	35.70082572857867	38.0	34.0	40.0	28.0	41.0
36	35.56786570181552	38.0	34.0	40.0	27.0	41.0
37	35.450999095668095	38.0	34.0	40.0	27.0	41.0
38	35.31327466666564	38.0	34.0	40.0	26.0	41.0
39	35.10700710310555	38.0	34.0	40.0	26.0	41.0
40	34.92550294700047	38.0	33.0	40.0	25.0	41.0
41	34.765281797617476	38.0	33.0	40.0	24.0	41.0
42	34.5530197944791	38.0	33.0	40.0	24.0	41.0
43	34.32229858930753	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	9.0
14	16.0
15	59.0
16	121.0
17	280.0
18	626.0
19	1401.0
20	2725.0
21	5173.0
22	9249.0
23	14726.0
24	22681.0
25	33755.0
26	48365.0
27	66730.0
28	89585.0
29	117245.0
30	147527.0
31	183470.0
32	226165.0
33	278055.0
34	348703.0
35	442609.0
36	594518.0
37	843030.0
38	1124116.0
39	606212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.056564433695996	22.92553386597183	14.61628116214619	22.401620538185984
2	22.760587875269415	25.089016870811896	29.46172759464422	22.688667659274472
3	24.07122578742074	24.5753970639268	28.96823392563968	22.38514322301278
4	18.979735398899862	18.609956024004646	30.507653021542076	31.902655555553423
5	15.343529936381817	37.83425856594738	32.263274465020174	14.558937032650626
6	34.60871849697695	35.37431831829986	13.420081852695489	16.596881332027692
7	28.73484370267933	31.131179209238663	20.48706405930758	19.646913028774424
8	28.50450696819513	34.36278784650133	18.19909858715664	18.9336065981469
9	26.774849778929354	13.595110116668224	18.94678076855345	40.68325933584897
10	17.50866451360521	28.661924861522376	31.983332946046012	21.8460776788264
11	35.81296442071387	22.031418668029918	20.033669031423855	22.121947879832362
12	21.555055261419902	26.77508023088982	29.220137122757343	22.449727384932935
13	32.32313436175081	19.600265711110413	24.160583535090822	23.916016392047947
14	21.975918154218068	21.57980964283979	26.783107640845998	29.661164562096143
15	25.876106337448118	27.864369035015063	23.09274596761881	23.16677865991801
16	23.5387281241076	25.337924192443516	26.352661787358517	24.770685896090363
17	23.83693296094857	26.230906334913147	26.577544492111727	23.354616212026556
18	24.175236438910357	24.081634534301717	27.317736984793434	24.425392041994492
19	24.794921760599205	24.58643955369905	28.495672976377328	22.12296570932441
20	24.132180330963582	24.236978359984782	28.439500311014125	23.19134099803751
21	23.664881368131834	24.84963489687947	28.561140537479268	22.92434319750943
22	23.70784145442841	24.88132204144333	27.962272709552366	23.44856379457589
23	23.340885116048884	24.85374462350775	28.183506591598224	23.62186366884514
24	23.186213441917175	25.186939749679237	28.251413102615075	23.375433705788513
25	23.880392359826157	24.388980632241115	28.230691630503312	23.49993537742942
26	23.2293463671841	25.280829719238458	28.340137107393875	23.149686806183563
27	23.8142718515029	25.072405125328416	28.19018969845169	22.92313332471699
28	23.114888560153435	25.725698024584997	28.235243056722485	22.92417035853908
29	23.292951108272284	25.87574145517738	28.11729006162477	22.71401737492556
30	23.407908227883958	25.506691844823635	27.939746030416966	23.145653896875437
31	23.35069852869866	25.348313734994456	28.473991287763635	22.826996448543248
32	23.063670611940214	25.676285283395416	27.870956120218338	23.38908798444603
33	22.91470262383	25.809025612622925	28.023400092065557	23.25287167148151
34	23.43982582440828	25.886399858348863	27.84683548168973	22.82693883555313
35	23.221549409188388	26.13642103112273	27.859644769825543	22.782384789863343
36	23.330034669577017	26.12943065498864	27.693969014581654	22.846565660852683
37	23.274092456174277	26.09203982440329	27.686632960506874	22.947234758915563
38	23.269252965004526	26.212354952095758	27.765601165626013	22.7527909172737
39	22.967610553086626	26.82585647951215	27.40943766072824	22.797095306672986
40	23.41793288816416	26.69236718141313	27.395898608050956	22.493801322371755
41	23.19637253250765	26.564543160675523	27.115188915875237	23.123895390941588
42	23.13643581845686	26.76680316464314	26.837839981456295	23.258921035443702
43	23.34142283728997	26.494082473763527	26.9224926682669	23.242002020679607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	763.0
1	886.5
2	1010.0
3	1594.5
4	2179.0
5	2179.0
6	4305.0
7	6431.0
8	6083.5
9	5736.0
10	8503.5
11	11271.0
12	11271.0
13	28802.0
14	46333.0
15	71827.5
16	97322.0
17	78822.5
18	60323.0
19	60323.0
20	76704.5
21	93086.0
22	69847.0
23	46608.0
24	50925.0
25	55242.0
26	55242.0
27	65678.5
28	76115.0
29	90925.5
30	105736.0
31	120910.5
32	136085.0
33	136085.0
34	148406.0
35	160727.0
36	178791.0
37	196855.0
38	216615.5
39	236376.0
40	236376.0
41	258280.5
42	280185.0
43	302799.0
44	325413.0
45	343498.0
46	361583.0
47	361583.0
48	386941.0
49	412299.0
50	422224.0
51	432149.0
52	432203.0
53	432257.0
54	432257.0
55	409260.0
56	386263.0
57	351435.5
58	316608.0
59	289361.0
60	262114.0
61	262114.0
62	232710.5
63	203307.0
64	175009.5
65	146712.0
66	125278.0
67	103844.0
68	103844.0
69	89407.5
70	74971.0
71	63692.0
72	52413.0
73	42156.0
74	31899.0
75	31899.0
76	26180.0
77	20461.0
78	16228.5
79	11996.0
80	9291.5
81	6587.0
82	6587.0
83	5153.0
84	3719.0
85	2997.0
86	2275.0
87	1687.5
88	1100.0
89	1100.0
90	802.0
91	504.0
92	344.0
93	184.0
94	137.0
95	90.0
96	90.0
97	54.0
98	18.0
99	14.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5207159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.490581456174155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01726187436554	39.89627497111497
2	10.847904525380686	10.954340141350674
3	3.758659401692587	5.693306960615231
4	1.778197008359831	3.5912880358286894
5	1.0089811488915028	2.54720224429253
6	0.6696911422031776	2.0287857099532705
7	0.48310410428001976	1.7074544990273672
8	0.36143271805799243	1.4599158473626794
9	0.2725535223664795	1.2385247239990727
>10	1.6223749240404393	15.167275370610724
>50	0.11831976210344777	4.086392724244386
>100	0.055727923156347156	5.082725593724728
>500	0.0034448841441329686	1.2017703332359209
>1k	0.0021199260263594553	2.1277860304483753
>5k	7.571164379838402E-5	0.2875071411115968
>10k+	1.5142328759676805E-4	2.929449673079968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54726	1.050976165698032	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	52676	1.0116072891186922	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23898	0.458945079264912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21965	0.42182310930010014	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7953	0.15273203679780087	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7089	0.13613949564436192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.344303102709174E-4	0.0	0.0	0.0	0.0
2	0.0011714641323608518	0.0	0.0	0.0	0.0
3	0.0017091853734445212	0.0	0.0	0.0	0.0
4	0.0018052070236380337	0.0	0.0	0.0	1.9204330038702488E-5
5	0.005665277361417234	0.0	0.0	3.4567794069664473E-4	3.8408660077404975E-5
6	0.007547301705210077	0.0	0.0	5.761299011610746E-4	5.761299011610746E-5
7	0.0085075182071452	0.0	0.0	6.529472213158845E-4	7.681732015480995E-5
8	0.010716016161595987	0.0	0.0	0.001113851142244744	7.681732015480995E-5
9	0.011311350392795764	0.0	0.0	0.0012482814525156616	9.602165019351243E-5
10	0.016534928163322842	0.0	0.0	0.0019588416639476536	9.602165019351243E-5
11	0.017783209615838503	0.0	0.0	0.002131680634295976	9.602165019351243E-5
12	0.018436156837154386	0.0	0.0	0.002227702284489488	9.602165019351243E-5
13	0.019607620969515238	0.0	0.0	0.0024581542449539184	9.602165019351243E-5
14	0.021624075623579	0.0	0.0	0.0029382624959214806	1.344303102709174E-4
15	0.023486895637333142	0.0	0.0	0.003437575076927745	1.536346403096199E-4
16	0.02494642472027453	0.0	0.0	0.0038024573476630923	2.3045196046442984E-4
17	0.02533051132104858	0.0	0.0	0.003840866007740497	2.3045196046442984E-4
18	0.02586823256213225	0.0	0.0	0.004052113638166224	2.688606205418348E-4
19	0.026982083704376995	0.0	0.0	0.0043593829187854646	2.688606205418348E-4
20	0.03404927715861951	0.0	1.9204330038702488E-5	0.0065486765431975475	2.688606205418348E-4
21	0.0494319455196202	0.0	1.9204330038702488E-5	0.011522598023221493	2.880649505805373E-4
22	0.050411366351594024	0.0	1.9204330038702488E-5	0.012021910604227757	3.072692806192398E-4
23	0.05131396986341304	0.0	1.9204330038702488E-5	0.012770879475737153	3.2647361065794227E-4
24	0.0533112201874381	0.0	1.9204330038702488E-5	0.013999956598214113	3.2647361065794227E-4
25	0.05430984534945063	0.0	1.9204330038702488E-5	0.014652903819529998	3.2647361065794227E-4
26	0.060186370341293594	0.0	1.9204330038702488E-5	0.01791763992610942	3.2647361065794227E-4
27	0.061549877774041466	0.0	1.9204330038702488E-5	0.019492394989283023	3.2647361065794227E-4
28	0.06437291428973073	0.0	1.9204330038702488E-5	0.023371669657100927	3.4567794069664473E-4
29	0.06462257058023387	0.0	1.9204330038702488E-5	0.029305807639059996	3.4567794069664473E-4
30	0.06573642172247861	0.0	1.9204330038702488E-5	0.03721799161500542	3.4567794069664473E-4
31	0.06754162874611665	0.0	1.9204330038702488E-5	0.06035920931164192	3.4567794069664473E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27900	0.0	12.552151	1
ATACTGT	1080	0.0	11.134259	6
TAGACAG	805	0.0	10.801243	5
CGTACGT	120	0.007168096	10.791667	10
TATATCG	155	3.600789E-4	10.741936	4
AACGCAG	38020	0.0	10.6465025	5
TATACTG	755	0.0	10.536425	5
TTACACT	705	0.0	10.496454	4
GTATAGA	355	4.5474735E-11	10.422535	1
TACACTC	765	0.0	10.398693	5
TACGCTG	395	3.6379788E-12	10.303798	5
GTATCAA	34180	0.0	10.278379	2
TTACACC	900	0.0	10.277778	4
GGGTAAG	2995	0.0	10.191986	1
TAAGGTG	680	0.0	10.066176	5
CTACACT	1105	0.0	10.045248	4
TTTACAC	800	0.0	9.94375	3
TACTATA	810	0.0	9.592592	2
GTTACAC	485	0.0	9.536082	3
ATACCGT	350	4.083631E-9	9.514286	6
>>END_MODULE