FastQCFastQC Report
Thu 9 Feb 2017
SRR2727590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10023437
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT758920.7571454781428766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT757260.7554893595879337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT503610.5024324490691168No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306060.30534436441312496No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105890.10564240589330785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA434350.013.2291921
TTAATCC33250.011.01654154
TAATCCC32100.011.0077895
TCTATAC8350.010.8562873
GTATAGA8750.010.7828571
TCGTTAC18050.010.55678723
TTTAATC40400.010.0742583
GTATCAA571400.010.0594162
TCTACAC18200.09.859893
CCTTTAA43000.09.7662791
TCAATAC11200.09.7455363
TAAGACT12350.09.7368424
TAGGGTG12200.09.7049185
AACGCAG622050.09.6775185
CGTTATG5500.09.41818124
CGTTACG15350.09.40065124
CTCGTTA21300.09.29342722
CCGATTT14200.09.2521
GTACTAC7600.09.251
GGAGAGT31500.09.2206346
Produced by FastQC (version 0.11.5)