##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10023437
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8567491370475	31.0	31.0	34.0	30.0	34.0
2	32.037836522542115	33.0	31.0	34.0	30.0	34.0
3	32.100899422024604	33.0	31.0	34.0	30.0	34.0
4	35.77095700806021	37.0	35.0	37.0	35.0	37.0
5	35.67361245449041	37.0	35.0	37.0	33.0	37.0
6	35.72442825749292	37.0	35.0	37.0	33.0	37.0
7	35.63662085170985	37.0	35.0	37.0	33.0	37.0
8	35.618415120482126	37.0	35.0	37.0	33.0	37.0
9	37.263551614082076	39.0	37.0	39.0	34.0	39.0
10	37.14279483175282	39.0	37.0	39.0	33.0	39.0
11	37.21092814770024	39.0	37.0	39.0	33.0	39.0
12	37.056724355128885	39.0	37.0	39.0	33.0	39.0
13	37.08919625074712	39.0	37.0	39.0	33.0	39.0
14	38.20155890639109	40.0	37.0	41.0	33.0	41.0
15	38.20018841840379	40.0	37.0	41.0	33.0	41.0
16	38.120031981046026	40.0	37.0	41.0	33.0	41.0
17	38.109054409181205	40.0	37.0	41.0	33.0	41.0
18	38.04989795416483	40.0	37.0	41.0	33.0	41.0
19	38.05642864817727	40.0	37.0	41.0	33.0	41.0
20	37.977257601359696	40.0	37.0	41.0	33.0	41.0
21	37.879305471765825	40.0	37.0	41.0	32.0	41.0
22	37.79042957021628	40.0	37.0	41.0	32.0	41.0
23	37.683249667753685	40.0	37.0	41.0	32.0	41.0
24	37.57099106823338	40.0	37.0	41.0	32.0	41.0
25	37.804939064314965	40.0	37.0	41.0	32.0	41.0
26	37.732951082547835	40.0	37.0	41.0	32.0	41.0
27	37.67524732284944	40.0	37.0	41.0	32.0	41.0
28	37.59769119115529	40.0	37.0	41.0	32.0	41.0
29	37.517754139622966	40.0	37.0	41.0	32.0	41.0
30	37.395604222384	40.0	36.0	41.0	31.0	41.0
31	37.27076540711534	39.0	36.0	41.0	31.0	41.0
32	37.144872761708385	39.0	36.0	41.0	31.0	41.0
33	37.041560694201	39.0	35.0	41.0	31.0	41.0
34	36.93295872463707	39.0	35.0	41.0	30.0	41.0
35	36.789323861665416	39.0	35.0	41.0	30.0	41.0
36	36.67092664921224	39.0	35.0	41.0	30.0	41.0
37	36.57986187771719	39.0	35.0	41.0	30.0	41.0
38	36.45829499402251	39.0	35.0	41.0	30.0	41.0
39	36.30109592148881	39.0	35.0	41.0	30.0	41.0
40	36.154075293734074	39.0	35.0	41.0	29.0	41.0
41	36.016116926758755	39.0	35.0	40.0	28.0	41.0
42	35.83791168638063	38.0	35.0	40.0	27.0	41.0
43	35.65769216686851	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	6.0
14	16.0
15	40.0
16	132.0
17	285.0
18	594.0
19	1364.0
20	2843.0
21	5501.0
22	10087.0
23	16759.0
24	26832.0
25	41294.0
26	61293.0
27	87692.0
28	121994.0
29	161875.0
30	211355.0
31	267553.0
32	334936.0
33	419126.0
34	532988.0
35	675907.0
36	890313.0
37	1277082.0
38	1904295.0
39	2971270.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.195213378405036	18.69569290453963	12.7369085075309	25.37218520952444
2	19.696976196887356	22.51060190232153	32.954604293916354	24.83781760687477
3	20.80499932308648	23.288119634013764	30.10359620158235	25.803284841317403
4	15.594600933791472	16.226300419706334	32.86485464017981	35.31424400632238
5	15.640273890083812	36.16248598160492	32.59894784593349	15.598292282377791
6	34.932369006758854	33.91598111506063	14.256018170214467	16.89563170796604
7	29.305736146194167	30.74873419167497	20.36848238782765	19.577047274303215
8	28.077315196374258	33.518622404670175	18.910808737561776	19.49325366139379
9	27.390933868292883	13.999828601706183	19.080999860626648	39.52823766937429
10	17.745819123719738	27.352942907707206	31.637491211846797	23.26374675672626
11	35.572927729280885	21.980234923410006	20.36359384510523	22.083243502203885
12	22.259550291980684	25.960905425953197	28.74918054555538	23.03036373651074
13	32.20174876142784	19.708918208394984	23.663060884205684	24.426272145971488
14	22.687946260349616	21.22983363890051	25.884524440069807	30.19769566068006
15	26.144944094525663	27.353401832126046	22.85137323654551	23.650280836802786
16	23.99797594378056	25.534873916003065	25.388357306979632	25.07879283323674
17	24.34719747328187	25.620882338064277	25.680791927958445	24.35112826069541
18	24.549034428011073	24.11824407136993	26.628869917574182	24.703851583044816
19	25.561611251709373	24.325488352947197	27.402466838470673	22.710433556872758
20	24.74996351052039	24.190434877776955	27.35948756898457	23.70011404271808
21	24.115510478092496	24.954583941616036	27.613831463199702	23.316074117091773
22	24.41031953410791	24.676685252773076	26.73391372639944	24.179081486719575
23	23.92012839508045	24.679917676940555	27.036025666645084	24.36392826133391
24	23.65982846003821	24.97169384114451	27.208242043123533	24.16023565569375
25	24.136501281945506	24.31369599070658	27.085230345638927	24.464572381708987
26	23.73323641381694	25.167903983434027	27.253106893374003	23.845752709375038
27	24.53639405325738	24.899363362088273	27.079882878497664	23.48435970615668
28	23.610374365599345	25.56371631806535	26.837840154030996	23.988069162304306
29	23.734812719429474	25.5504174865368	27.08529020534573	23.629479588687992
30	23.84674039453732	25.352870477461977	27.002723716425812	23.797665411574894
31	23.831017244883167	24.95326702806632	27.213818972474215	24.0018967545763
32	23.422744114618567	25.50467469391986	26.82540928825113	24.247171903210447
33	23.434297038031964	25.27339674006032	27.15209363814029	24.140212583767422
34	24.053565658166953	25.510181786945935	27.007512492970225	23.428740061916887
35	23.83686354291447	25.811256158940292	26.791857922586832	23.560022375558404
36	23.63337046963033	25.629981013498664	26.978879599881754	23.757768916989253
37	23.641481459902426	25.68178958973853	26.7683131045768	23.90841584578224
38	23.61252931504433	25.60639628901743	26.98602285822717	23.795051537711068
39	23.324125247657065	26.063544869888446	26.964253878185694	23.648076004268795
40	23.944940243551187	25.657536431864642	26.968863075609693	23.428660248974477
41	23.526002108857472	25.741210325360452	26.92788910630156	23.804898459480516
42	23.370496567195463	25.796819992982446	26.797404922084112	24.035278517737975
43	23.445301247466315	25.412460815586506	26.769709831068923	24.372528105878253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1708.0
1	2043.5
2	2379.0
3	5334.5
4	8290.0
5	8290.0
6	11629.0
7	14968.0
8	13198.5
9	11429.0
10	16322.5
11	21216.0
12	21216.0
13	44932.5
14	68649.0
15	103093.0
16	137537.0
17	121606.5
18	105676.0
19	105676.0
20	124110.0
21	142544.0
22	109801.5
23	77059.0
24	88708.5
25	100358.0
26	100358.0
27	118106.0
28	135854.0
29	161614.0
30	187374.0
31	212545.5
32	237717.0
33	237717.0
34	262849.5
35	287982.0
36	321940.5
37	355899.0
38	390799.5
39	425700.0
40	425700.0
41	470976.0
42	516252.0
43	559286.0
44	602320.0
45	641535.5
46	680751.0
47	680751.0
48	736804.5
49	792858.0
50	821141.5
51	849425.0
52	851220.5
53	853016.0
54	853016.0
55	809578.0
56	766140.0
57	705888.0
58	645636.0
59	591914.0
60	538192.0
61	538192.0
62	479848.5
63	421505.0
64	366239.0
65	310973.0
66	268213.0
67	225453.0
68	225453.0
69	193953.5
70	162454.0
71	142166.0
72	121878.0
73	100645.0
74	79412.0
75	79412.0
76	67028.5
77	54645.0
78	44548.5
79	34452.0
80	27097.5
81	19743.0
82	19743.0
83	15705.0
84	11667.0
85	9652.0
86	7637.0
87	5859.5
88	4082.0
89	4082.0
90	2868.0
91	1654.0
92	1138.0
93	622.0
94	436.0
95	250.0
96	250.0
97	154.0
98	58.0
99	40.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0023437E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.548263312623824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52221522605431	36.550727502328954
2	11.027629903554505	10.26634040929639
3	3.740576664423699	5.223520425499514
4	1.8041316460841377	3.3591677965024753
5	1.0710182816855378	2.4927020494266166
6	0.6993863463158251	1.9533131883337762
7	0.4769762759468972	1.5541692100655622
8	0.3661065446864369	1.3633299074031315
9	0.2795309198666256	1.1710510975774473
>10	1.758948925322235	15.47523724655708
>50	0.15408014294543937	4.9244048082404035
>100	0.08912220913542647	7.910019846079135
>500	0.006582334709544072	2.0822943752887886
>1k	0.0034810199382430142	2.91159682930414
>5k	1.0677966567370943E-4	0.34477732092663205
>10k+	1.0677966567370943E-4	2.4173479871698764
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	75892	0.7571454781428766	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	75726	0.7554893595879337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50361	0.5024324490691168	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30606	0.30534436441312496	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10589	0.10564240589330785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.995323560172025E-5	0.0	0.0	2.992985340258037E-5	2.992985340258037E-5
2	5.187841256447265E-4	0.0	0.0	2.992985340258037E-5	2.992985340258037E-5
3	7.18316481661929E-4	0.0	0.0	2.992985340258037E-5	2.992985340258037E-5
4	7.781761884670897E-4	0.0	0.0	6.983632460602087E-5	2.992985340258037E-5
5	0.0028233828376434153	0.0	0.0	1.59625884813762E-4	2.992985340258037E-5
6	0.00399064712034405	0.0	0.0	1.7957912041548224E-4	4.9883089004300624E-5
7	0.004609197423997378	0.0	0.0	1.8955573821634235E-4	4.9883089004300624E-5
8	0.005866251266905753	0.0	0.0	2.5939206282236325E-4	5.985970680516074E-5
9	0.006305222450143599	0.0	0.0	2.5939206282236325E-4	6.983632460602087E-5
10	0.009298207790401636	0.0	0.0	3.591582408309645E-4	6.983632460602087E-5
11	0.010265939717085068	0.0	0.0	4.190179476361252E-4	6.983632460602087E-5
12	0.010794700460530654	0.0	0.0	4.888542722421461E-4	6.983632460602087E-5
13	0.011582853266798605	0.0	0.0	5.088075078438664E-4	6.983632460602087E-5
14	0.012989556376719882	0.0	0.0	5.786438324498872E-4	1.0974279580946136E-4
15	0.013937335067801593	0.0	0.0	6.784100104584884E-4	1.2969603141118163E-4
16	0.014845207287679865	0.0	0.0	7.18316481661929E-4	1.696025026146221E-4
17	0.015194388910709969	0.0	0.0	7.582229528653694E-4	1.696025026146221E-4
18	0.015423851120129752	0.0	0.0	8.280592774713903E-4	1.8955573821634235E-4
19	0.01604240142378308	0.0	0.0	8.579891308739707E-4	1.995323560172025E-4
20	0.020302417224750353	0.0	0.0	0.0013368667853152565	1.995323560172025E-4
21	0.02900202794710038	0.0	0.0	0.0021549494449857867	2.095089738180626E-4
22	0.02960062501515199	0.0	0.0	0.0024841778324141708	2.095089738180626E-4
23	0.030298988261212197	0.0	0.0	0.0027934529842408348	2.3943882722064297E-4
24	0.031595948575324016	0.0	0.0	0.0035117694659027635	2.494154450215031E-4
25	0.03225440535018078	0.0	0.0	0.004100389916153511	2.494154450215031E-4
26	0.03723273763280998	0.0	0.0	0.005457209937070488	2.494154450215031E-4
27	0.03822042279509513	0.0	0.0	0.008300546010315624	2.494154450215031E-4
28	0.04008605032385598	0.0	0.0	0.014795324198675564	2.5939206282236325E-4
29	0.04031551253327576	0.0	0.0	0.028612939852866837	2.5939206282236325E-4
30	0.04092408621912823	0.0	0.0	0.04761839676350537	2.5939206282236325E-4
31	0.04201153755942198	0.0	0.0	0.10164178215516294	2.5939206282236325E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	43435	0.0	13.229192	1
TTAATCC	3325	0.0	11.0165415	4
TAATCCC	3210	0.0	11.007789	5
TCTATAC	835	0.0	10.856287	3
GTATAGA	875	0.0	10.782857	1
TCGTTAC	1805	0.0	10.556787	23
TTTAATC	4040	0.0	10.074258	3
GTATCAA	57140	0.0	10.059416	2
TCTACAC	1820	0.0	9.85989	3
CCTTTAA	4300	0.0	9.766279	1
TCAATAC	1120	0.0	9.745536	3
TAAGACT	1235	0.0	9.736842	4
TAGGGTG	1220	0.0	9.704918	5
AACGCAG	62205	0.0	9.677518	5
CGTTATG	550	0.0	9.418181	24
CGTTACG	1535	0.0	9.400651	24
CTCGTTA	2130	0.0	9.293427	22
CCGATTT	1420	0.0	9.25	21
GTACTAC	760	0.0	9.25	1
GGAGAGT	3150	0.0	9.220634	6
>>END_MODULE