FastQCFastQC Report
Thu 9 Feb 2017
SRR2727589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135958
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316700.7657234430330289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT305690.7391032500813596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141020.340960909177511No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120660.29173410368287106No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.11738513785681576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT852.7258875E-517.4117645
GGTATCA139200.015.3635061
TAGCGTA1102.4587804E-413.45454519
ATACGCG1254.8503312E-513.3200014
TAACACG1153.5806693E-412.8695654
TGTATAC4500.012.3333333
CGTTCGG1205.126439E-412.33333310
CGCGTAT1205.126439E-412.3333337
TACACTC4200.012.3333335
ATACCGA1501.9463088E-512.3333336
GTATCAA174600.012.2379722
TACACGG3500.012.1571435
TATACAG9000.012.1277785
TTGCGAT1100.003747868111.77272727
CGCGAAC2103.8484723E-711.4523812
CTACACT7600.011.4407894
CTACACG3451.8189894E-1211.260874
ATACACT8750.011.2057144
GGGTAAG27450.011.1202181
TTATACG1502.657981E-411.12
Produced by FastQC (version 0.11.5)