##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727589.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4135958
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.080904835107127	31.0	31.0	33.0	30.0	34.0
2	31.32081539512732	31.0	31.0	34.0	30.0	34.0
3	31.321946209318373	31.0	31.0	34.0	28.0	34.0
4	35.131528898504286	37.0	35.0	37.0	32.0	37.0
5	34.938213830991515	35.0	35.0	37.0	32.0	37.0
6	35.09124705811809	36.0	35.0	37.0	32.0	37.0
7	35.0485848744112	36.0	35.0	37.0	32.0	37.0
8	35.043292025692715	37.0	35.0	37.0	32.0	37.0
9	36.624867805717564	38.0	35.0	39.0	32.0	39.0
10	36.34570660533787	38.0	35.0	39.0	32.0	39.0
11	36.50638763739864	38.0	35.0	39.0	32.0	39.0
12	36.29400757938064	38.0	35.0	39.0	32.0	39.0
13	36.38419684145729	38.0	35.0	39.0	32.0	39.0
14	37.30827319813209	39.0	36.0	40.0	32.0	41.0
15	37.31000895076787	39.0	36.0	40.0	32.0	41.0
16	37.1952307059211	39.0	36.0	40.0	32.0	41.0
17	37.157689222182626	39.0	36.0	40.0	31.0	41.0
18	37.07687215392419	39.0	36.0	40.0	31.0	41.0
19	37.07184792495475	39.0	36.0	40.0	31.0	41.0
20	36.97637693612943	39.0	36.0	40.0	31.0	41.0
21	36.86851994145008	39.0	36.0	40.0	31.0	41.0
22	36.76561826788377	39.0	36.0	40.0	30.0	41.0
23	36.64290643183514	38.0	35.0	40.0	30.0	41.0
24	36.50742584910195	38.0	35.0	40.0	30.0	41.0
25	36.77196697838808	39.0	36.0	40.0	30.0	41.0
26	36.66973576617558	39.0	36.0	40.0	30.0	41.0
27	36.59615789135189	39.0	35.0	40.0	30.0	41.0
28	36.50289703135283	38.0	35.0	40.0	30.0	41.0
29	36.404277557944255	38.0	35.0	40.0	30.0	41.0
30	36.287724633567365	38.0	35.0	40.0	30.0	41.0
31	36.146019616253355	38.0	35.0	40.0	30.0	41.0
32	36.01752749907035	38.0	35.0	40.0	29.0	41.0
33	35.90961441097806	38.0	35.0	40.0	29.0	41.0
34	35.796748177810315	38.0	34.0	40.0	28.0	41.0
35	35.66724057642752	38.0	34.0	40.0	27.0	41.0
36	35.5464332568174	38.0	34.0	40.0	27.0	41.0
37	35.441379723875336	38.0	34.0	40.0	27.0	41.0
38	35.3223949566219	38.0	34.0	40.0	26.0	41.0
39	35.13142203088136	38.0	34.0	40.0	26.0	41.0
40	34.980348930042325	38.0	33.0	40.0	25.0	41.0
41	34.84705067121088	38.0	33.0	40.0	25.0	41.0
42	34.66533920315438	38.0	33.0	40.0	24.0	41.0
43	34.46337462807891	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	19.0
15	49.0
16	109.0
17	241.0
18	547.0
19	1110.0
20	2294.0
21	4228.0
22	7471.0
23	12240.0
24	18711.0
25	28011.0
26	39181.0
27	53934.0
28	72196.0
29	93552.0
30	118607.0
31	148766.0
32	181765.0
33	224824.0
34	281569.0
35	357817.0
36	482051.0
37	677749.0
38	886284.0
39	442624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.4526491806735	23.332127647331042	14.625898038616448	22.589325133379017
2	23.3792509498404	24.89638434432845	29.532456567498993	22.19190813833216
3	24.4752485397579	24.586758376173066	28.648985313680654	22.289007770388384
4	19.92839385699758	18.86063639911237	30.658362584919868	30.552607158970186
5	15.631130683628799	37.62497104661121	32.43398506464524	14.309913205114752
6	34.86718192012589	35.6035530341459	13.825333816252487	15.703931229475735
7	29.089246070680602	31.122946606324337	20.42963202237547	19.35817530061959
8	28.158240485033936	34.65470394041719	18.53099088530396	18.65606468924491
9	27.279411444700358	13.753959783924305	18.757250436295532	40.209378335079805
10	17.26439194982154	28.4782872553348	32.387224435064375	21.870096359779282
11	36.360499792309305	22.031340743788984	20.200809582689185	21.407349881212525
12	21.542699418127555	26.35251615224333	29.16436772326992	22.940416706359205
13	32.00494299023346	19.978152582787352	23.83467143525152	24.182232991727673
14	22.17070386111271	21.48866115178152	26.041028463054992	30.299606524050777
15	25.89634614277998	28.210803881470746	22.426001424579265	23.46684855117001
16	24.03136105347298	26.00616350552883	25.51391962877766	24.44855581222053
17	24.041467539080426	26.427541092051708	25.971105122440797	23.559886246427066
18	24.220869747710204	24.979243019392364	26.63126656508601	24.168620667811425
19	25.202020910270367	24.9520425497551	27.49870767546479	22.347228864509745
20	24.295241876247292	24.803685143804653	27.576561464115446	23.324511515832608
21	24.217243018425236	24.94848835505583	27.705286175536596	23.128982450982335
22	24.19536175173926	24.980113434420755	27.105255904436167	23.71926890940382
23	23.932472234969502	25.034248413547722	27.32264205777718	23.710637293705595
24	23.621734069833398	25.292229756685153	27.277041981567514	23.80899419191394
25	24.159336240841906	24.8827720204122	27.297956120444162	23.659935618301734
26	23.7295446423779	25.348153922259364	27.48393479817735	23.438366637185386
27	24.260715413454392	25.56616870867644	26.98569472900837	23.187421148860796
28	23.59673381596235	26.024369686539373	27.154700313687908	23.224196183810378
29	23.774516085511507	26.116222650230007	27.221818983655055	22.88744228060343
30	24.162455228026978	25.654104804739315	26.81765627213816	23.365783695095548
31	24.03027303468749	25.37180019719736	27.504776402468305	23.093150365646846
32	23.700047244193488	25.772239466648355	26.99420545373043	23.53350783542773
33	23.658170610049716	25.600598458688413	27.041062796092223	23.70016813516965
34	23.96733719249567	25.771320695229498	27.153588116707184	23.107753995567652
35	23.835372602913278	26.017333831726532	26.97162301938269	23.175670545977496
36	23.869028650677787	25.952439555720826	26.965747717941042	23.21278407566034
37	23.746155062503053	26.17328319098018	27.057286365093645	23.02327538142312
38	23.97183433680903	25.895040520237387	26.998146499553428	23.13497864340015
39	23.365807873290784	26.72921243397539	26.884726585714848	23.020253107018977
40	24.117749745040932	26.187113118653528	26.902352490039792	22.79278464626575
41	23.642769099686216	26.25386911569218	26.68590928631287	23.417452498308737
42	23.726909219097486	26.34124911326469	26.394513677363264	23.537327990274562
43	23.757784774410183	26.126885234327812	26.527227790997877	23.58810220026412
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	903.5
2	937.0
3	1292.5
4	1648.0
5	1648.0
6	3083.0
7	4518.0
8	4146.5
9	3775.0
10	5542.0
11	7309.0
12	7309.0
13	17590.0
14	27871.0
15	41658.5
16	55446.0
17	45814.5
18	36183.0
19	36183.0
20	47839.0
21	59495.0
22	47094.5
23	34694.0
24	39705.0
25	44716.0
26	44716.0
27	54452.5
28	64189.0
29	74717.0
30	85245.0
31	97806.5
32	110368.0
33	110368.0
34	122613.0
35	134858.0
36	149866.5
37	164875.0
38	180647.0
39	196419.0
40	196419.0
41	212435.0
42	228451.0
43	244672.0
44	260893.0
45	275132.0
46	289371.0
47	289371.0
48	307651.0
49	325931.0
50	335157.5
51	344384.0
52	341538.0
53	338692.0
54	338692.0
55	320910.5
56	303129.0
57	279996.0
58	256863.0
59	236002.5
60	215142.0
61	215142.0
62	189498.0
63	163854.0
64	141600.5
65	119347.0
66	101463.5
67	83580.0
68	83580.0
69	71487.0
70	59394.0
71	51443.0
72	43492.0
73	35464.5
74	27437.0
75	27437.0
76	22827.0
77	18217.0
78	14576.0
79	10935.0
80	8443.5
81	5952.0
82	5952.0
83	4713.0
84	3474.0
85	2849.0
86	2224.0
87	1623.0
88	1022.0
89	1022.0
90	749.5
91	477.0
92	336.0
93	195.0
94	133.5
95	72.0
96	72.0
97	42.5
98	13.0
99	7.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4135958.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.01474420514163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30330403645162	46.10198522839838
2	9.3066907582625	10.42623804440859
3	3.0779721575761596	5.172354692315293
4	1.48925162874081	3.336801961640281
5	0.8831002018208285	2.473331595625133
6	0.5625505692434608	1.890667574317756
7	0.412411522383123	1.6170788153480573
8	0.28538894360882233	1.2788790940179018
9	0.2176068527586675	1.097027297510641
>10	1.2993565410132195	13.518910056628316
>50	0.10511654479204338	4.086131833830117
>100	0.05410851601242092	5.256566527754386
>500	0.0021088307234819388	0.8061952478330995
>1k	8.607471774992103E-4	0.8065809221038994
>5k	0.0	0.0
>10k+	1.7214943549983606E-4	2.1312511082682137
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31670	0.7657234430330289	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30569	0.7391032500813596	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14102	0.340960909177511	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12066	0.29173410368287106	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4855	0.11738513785681576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.6596014756436116E-4	0.0	0.0	2.417819523312374E-5	0.0
3	3.626729284968561E-4	0.0	0.0	4.835639046624748E-5	0.0
4	4.110293189631036E-4	0.0	0.0	7.253458569937123E-5	2.417819523312374E-5
5	0.0017891864472511568	0.0	0.0	3.384947332637324E-4	2.417819523312374E-5
6	0.002538710499477993	0.0	0.0	3.8685112372997983E-4	1.208909761656187E-4
7	0.0028530270375086014	0.0	0.0	5.077420998955986E-4	1.208909761656187E-4
8	0.0037717984563673036	0.0	0.0	6.52811271294341E-4	1.208909761656187E-4
9	0.003989402213465418	0.0	0.0	7.737022474599597E-4	1.208909761656187E-4
10	0.005657697684550956	0.0	0.0	0.0014506917139874244	1.208909761656187E-4
11	0.0060445488082809355	0.0	0.0	0.0015957608853861668	1.208909761656187E-4
12	0.006286330760612173	0.0	0.0	0.0016924736663186618	1.692473666318662E-4
13	0.006697360079575276	0.0	0.0	0.0018133646424842804	2.417819523312374E-4
14	0.00730181496040337	0.0	0.0	0.002345284937613003	2.901383427974849E-4
15	0.00795462623169771	0.0	0.0	0.002538710499477993	3.384947332637324E-4
16	0.008438190136360185	0.0	0.0	0.0026596014756436115	5.077420998955986E-4
17	0.008607437502992052	0.0	0.0	0.0027563142565761064	5.319202951287223E-4
18	0.008704150283924546	0.0	0.0	0.002828848842275478	6.286330760612173E-4
19	0.009066823212421403	0.0	0.0	0.0030222744041404678	6.286330760612173E-4
20	0.011702246492831891	0.0	0.0	0.0039410458229991695	6.286330760612173E-4
21	0.01719069681075098	0.0	0.0	0.006068727003514059	6.52811271294341E-4
22	0.017480835153548466	0.0	0.0	0.006407221736777791	6.52811271294341E-4
23	0.01777097349634595	0.0	0.0	0.006576469103409657	6.52811271294341E-4
24	0.018713923110437774	0.0	0.0	0.007060033008072133	6.52811271294341E-4
25	0.0192458434055665	0.0	0.0	0.007374349546102741	6.769894665274648E-4
26	0.021857088490743862	0.0	0.0	0.009066823212421403	7.011676617605884E-4
27	0.022316474200173214	0.0	0.0	0.00996141643604698	7.253458569937122E-4
28	0.023477027571363152	0.0	0.0	0.012355057764126232	7.253458569937122E-4
29	0.023622096742761896	0.0	0.0	0.0162961035871254	7.737022474599597E-4
30	0.023936413280792504	0.0	0.0	0.021832910295510738	7.737022474599597E-4
31	0.024419977185454977	0.0	0.0	0.03994237852512042	7.737022474599597E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGT	85	2.7258875E-5	17.411764	5
GGTATCA	13920	0.0	15.363506	1
TAGCGTA	110	2.4587804E-4	13.454545	19
ATACGCG	125	4.8503312E-5	13.320001	4
TAACACG	115	3.5806693E-4	12.869565	4
TGTATAC	450	0.0	12.333333	3
CGTTCGG	120	5.126439E-4	12.333333	10
CGCGTAT	120	5.126439E-4	12.333333	7
TACACTC	420	0.0	12.333333	5
ATACCGA	150	1.9463088E-5	12.333333	6
GTATCAA	17460	0.0	12.237972	2
TACACGG	350	0.0	12.157143	5
TATACAG	900	0.0	12.127778	5
TTGCGAT	110	0.0037478681	11.772727	27
CGCGAAC	210	3.8484723E-7	11.45238	12
CTACACT	760	0.0	11.440789	4
CTACACG	345	1.8189894E-12	11.26087	4
ATACACT	875	0.0	11.205714	4
GGGTAAG	2745	0.0	11.120218	1
TTATACG	150	2.657981E-4	11.1	2
>>END_MODULE